Post-mortem changes in metabolomic profiles of human serum, aqueous humor and vitreous humor

Metabolomics ◽  
2020 ◽  
Vol 16 (7) ◽  
Author(s):  
Ekaterina A. Zelentsova ◽  
Lyudmila V. Yanshole ◽  
Arsenty D. Melnikov ◽  
Ivan S. Kudryavtsev ◽  
Vladimir P. Novoselov ◽  
...  
Keyword(s):  
Human Serum ◽  
Aqueous Humor ◽  
Vitreous Humor ◽  
Post Mortem

Thanatobiochemistry: post mortem study of the vitreous humor for the diagnosis of diabetic ketoacidosis death

2018 ◽  
Vol 76 (3) ◽  
pp. 245-250
Author(s):  
Guillaume Rousseau ◽  
Nicolas Bergerat ◽  
Guillaume Drevin ◽  
Pascal Reynier ◽  
Nathalie Jousset
Keyword(s):  
Diabetic Ketoacidosis ◽  
Vitreous Humor ◽  
Post Mortem

Comparison of Fluconazole Pharmacokinetics in Serum, Aqueous Humor, Vitreous Humor, and Cerebrospinal Fluid Following a Single Dose and at Steady State

1998 ◽  
Vol 14 (5) ◽  
pp. 459-471 ◽  
Author(s):  
UMAR K. MIAN ◽  
MARTIN MAYERS ◽  
YOGENDER GARG ◽  
QING-FENG LIU ◽  
GIRARD NEWCOMER ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Single Dose ◽  
Steady State ◽  
Aqueous Humor ◽  
Vitreous Humor

scholarly journals Modeling Approach To Characterize Intraocular Doripenem Pharmacokinetics after Intravenous Administration to Rabbits, with Tentative Extrapolation to Humans

2012 ◽  
Vol 56 (7) ◽  
pp. 3531-3534 ◽  
Author(s):  
Oudy Semoun ◽  
Sandrine Marchand ◽  
Nicolas Grégoire ◽  
Isabelle Lamarche ◽  
Christophe Adier ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
High Performance ◽  
Vitreous Humor ◽  
Time Curve ◽  
Time Profiles ◽  
Modeling Approach ◽  
New Zealand White Rabbits ◽  
Dosing Regimens ◽  
Sampling Times ◽  
Peripheral Elimination

ABSTRACTThe aim of this study was to determine the penetration of doripenem administered intravenously into the rabbit aqueous and vitreous humors. Nineteen New Zealand White rabbits received a 20-mg dose of doripenem intravenously over 60 min. Specimens of aqueous humor, vitreous humor, and blood were obtained 30 min (n= 5), 1 h (n= 5), 2 h (n= 5), and 3 h (n= 4) after the beginning of the infusion and analyzed by high-performance liquid chromatography (HPLC). A pharmacokinetic (PK) model was developed to fit the experimental data. Doripenem concentrations in aqueous humor were lower than those in plasma ultrafiltrates at all sampling times, with an average aqueous humor-to-plasma ultrafiltrate area under the concentration-time curve ratio estimated as 8.3%. A pharmacokinetic model with peripheral elimination described the data adequately and was tentatively used to predict concentration-versus-time profiles and pharmacokinetic-pharmacodynamic (PK-PD) target attainment in patients under various dosing regimens. In conclusion, systematically administered doripenem does not seem to be a promising approach for the treatment of intraocular infections, especially since it could not be detected in the vitreous humor. However, this study has provided an opportunity to develop a new PK modeling approach to characterize the intraocular distribution of doripenem administered intravenously to rabbits, with tentative extrapolation to humans.

Analysis of hypoxanthine and lactic acid levels in vitreous humor for the estimation of post-mortem interval (PMI) using LC–MS/MS

2019 ◽  
Vol 299 ◽  
pp. 135-141 ◽  
Author(s):  
Ahra Go ◽  
Geunae Shim ◽  
Jiwon Park ◽  
Jinwoo Hwang ◽  
Mihyun Nam ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Vitreous Humor ◽  
Post Mortem ◽  
Post Mortem Interval

scholarly journals “2n Analytical Platform” To Update Procedures in Thanatochemistry: Estimation of Post Mortem Interval in Vitreous Humor

2019 ◽  
Vol 91 (11) ◽  
pp. 7025-7031 ◽  
Author(s):  
Roberta Risoluti ◽  
Silvia Canepari ◽  
Paola Frati ◽  
Vittorio Fineschi ◽  
Stefano Materazzi
Keyword(s):  
Vitreous Humor ◽  
Post Mortem ◽  
Post Mortem Interval

scholarly journals Ethanol Determination in Post-Mortem Samples: Correlation between Blood and Vitreous Humor Concentration

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2724
Author(s):  
Fabio Savini ◽  
Angela Tartaglia ◽  
Ludovica Coccia ◽  
Danilo Palestini ◽  
Cristian D’Ovidio ◽  
...  
Keyword(s):  
Whole Blood ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Linear Regression Analysis ◽  
Vitreous Humor ◽  
Alcohol Concentration ◽  
Coefficient Of Determination ◽  
Post Mortem ◽  
Excessive Alcohol Consumption ◽  
Ethanol Determination

Ethanol (ethylic alcohol) represents the most commonly used drug worldwide and is often involved in clinical and forensic toxicology. Based on several reports, excessive alcohol consumption is the main contributing factor in traffic accidents, drownings, suicides, and other crimes. For these reasons, it becomes essential to analyze the alcohol concentration during autopsy. Although blood is usually used for alcohol analysis in post-mortem cases, it could suffer alterations, putrefaction, and microbial contaminations. As an alternative to whole blood, vitreous humor has been successfully used in medico-legal studies. In this work, post-mortem specimens were analyzed for ethanol determination. The analysis of blood and vitreous humor were carried-out using gas chromatography-flame ionized detector (GC-FID) with a total run time of 6 min. The method was validated in terms of limit of detection, limit of quantification, dynamic range, sensibility, recovery, precision and trueness. A linear regression analysis indicated a coefficient of determination (R2) of 0.9981. The study confirmed no statistically differences between alcohol concentration in blood and vitreous humor, leading vitreous humor as an excellent matrix that could be used as an alternative to whole blood in toxicological analysis in cases where blood is not available.

scholarly journals The Cellular Expression and Genetics of Purple Body (Pb) in the Ocular Media of the Guppy Poecilia reticulata

2017 ◽  
Author(s):  
Alan S. Bias ◽  
Richard D. Squire
Keyword(s):  
Aqueous Humor ◽  
Poecilia Reticulata ◽  
Vitreous Humor ◽  
Uv Damage ◽  
Media Study ◽  
Visual Component ◽  
Cellular Expression ◽  
Internal Structures ◽  
Ocular Media ◽  
Filter Mechanism

AbstractOur study revealed the presence of all major classes of chromatophores (melanophores, xanthophores, erythrophores, violet-blue iridophores, xantho-erythrophores) and crystalline platelets in various combinations in the iris and ocular media (cornea, aqueous humor, vitreous humor, outer lens membrane) of Poecilia reticulata. This novel ocular media study of P. reticulata takes into account the distinct interactions of Purple Body (Pb) based on results of previous Bias and Squire Purple Body (Pb) publications. Taken in conjunction with other researcher’s published results (regarding UV reflected color and pattern, vision, mate choice, individual preferences, and opsin capabilities) this indicates that these ocular chromatophore populations together create a complex ocular filter mechanism. This mechanism in turn provides spectral capabilities into the UV and Near-UV wavelengths in both Pb and non-Pb individuals. The chromatophores in the cornea, aqueous humor, covering membranes of the lens, and the vitreous humor comprise an ocular filter system that could reduce UV damage to the internal structures of the eye. The guppy’s ability to use UVA as a visual component provides a “private signally system” that cannot be detected by some predators. While non-Pb guppies should derive benefit in the near-UV from violet-blue iridophore units, greater benefit will be derived by Pb individuals with more violet iridophores functioning in the lower UV and near-UV wavelengths. To our knowledge little has been published for P. reticulata concerning pigmentation within the guppy eye. Macroscopic and microscopic imagery is presented.

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