scholarly journals Monitoring the abundance and the activity of ammonia-oxidizing bacteria in a full-scale nitrifying activated sludge reactor

2014 ◽  
Vol 22 (3) ◽  
pp. 2328-2334 ◽  
Author(s):  
Ö. Eyice ◽  
O. Ince ◽  
B. K. Ince
Keyword(s):  
Activated Sludge ◽  
Full Scale ◽  
Ammonia Oxidizing Bacteria ◽  
Activated Sludge Reactor

Quantification of ammonia-oxidizing bacteria populations in full-scale sewage activated sludge systems and assessment of system variables affecting their performance

2006 ◽  
Vol 54 (1) ◽  
pp. 91-99 ◽  
Author(s):  
T. Limpiyakorn ◽  
F. Kurisu ◽  
O. Yagi
Keyword(s):  
Activated Sludge ◽  
Real Time ◽  
Real Time Pcr ◽  
Full Scale ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Ammonia Oxidizing Bacteria ◽  
Primer Sets ◽  
Activated Sludge Systems ◽  
Pcr Primer

This study carried out quantification of ammonia-oxidizing bacteria (AOB) populations in 12 full-scale sewage activated sludge systems that were different in ammonia removals and treatment processes during three different seasons. Experiment was divided into 3 parts: 1) analysis of AOB communities by PCR-DGGE-cloning-sequencing of 16S rRNA genes; 2) development of four real-time PCR primer sets for quantification of the particular AOB of interest; and 3) quantification of AOB populations by using the newly developed real-time PCR primer sets. The results suggested that all the primer sets gave good reproducibility and specificity for PCR amplification with the detection limits of 102 copies/PCR reaction. Although the 12 systems were different in several aspects, one of the identified sequence types of Nitrosomonas oligotropha cluster was the dominant AOB in every system and every season studied. However, the other sequence type of this cluster was not significantly involved in ammonia removals in the systems. The occurrence of N. communis cluster in the systems seemed to depend on the remaining oxygen concentrations in the sludge floc and thus the activity of aerobic heterotrophs in the aeration tanks. N. europaea–Nitrosococcus. mobilis solely existed in one A2O system of which the influent contained twice the chloride concentrations than those of other systems.

Performance of nitrogen removal in an alternating activated sludge reactor for full-scale applications

2018 ◽  
pp. 1-11 ◽  
Author(s):  
Jun Li ◽  
Kunquan Li ◽  
Erin Berns ◽  
Hanting Wang ◽  
Nora Sadik ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Full Scale ◽  
Activated Sludge Reactor

The Polish perspective on adopting EU standards nitrogen removal at large WWTPs - case studies

2004 ◽  
Vol 50 (7) ◽  
pp. 27-34 ◽  
Author(s):  
J. Makinia ◽  
E. Dobiegala ◽  
M. Swinarski
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Full Scale ◽  
Accurate Information ◽  
Design Phase ◽  
Anoxic Zone ◽  
Oxygen Penetration ◽  
Batch Tests ◽  
Effluent Standards ◽  
Activated Sludge Reactor

The most challenging issue for existing large WWTPs (>100,000 PE) in Poland will be achievement of the new effluent standards for total nitrogen. Consequently, reliable and accurate information concerning the dimensioning of anoxic compartments is necessary. This study focused on validating to what extent the denitrification rates determined from batch tests were comparable with the rates calculated based on a mass balance over a full-scale activated sludge reactor. The experiments were conducted at two large WWTPs in northern Poland: “Wschod” in Gdansk and “Debogorze” in Gdynia. Two types of batch tests were used to determine the denitrification capability of activated sludge. Lower nitrate utilization rates observed during the full-scale experiments could potentially result from the local disturbances such as nitrate limitation (“Wschod” WWTP) or oxygen penetration to the anoxic zone (“Debogorze” WWTP). These factors should be taken into consideration during the design phase of the anoxic compartments.

Molecular assessment of ammonia- and nitrite-oxidizing bacteria in full-scale activated sludge wastewater treatment plants

2003 ◽  
Vol 48 (8) ◽  
pp. 119-126 ◽  
Author(s):  
K.G. Robinson ◽  
H.M. Dionisi ◽  
G. Harms ◽  
A.C. Layton ◽  
I.R. Gregory ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
16S Rdna ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Amoa Gene ◽  
Full Scale ◽  
Ammonia Oxidizing Bacteria ◽  
Copy Numbers ◽  
Nitrite Oxidizing Bacteria

Nitrification was assessed in two full-scale wastewater treatment plants (WWTPs) over time using molecular methods. Both WWTPs employed a complete-mix suspended growth, aerobic activated sludge process (with biomass recycle) for combined carbon and nitrogen treatment. However, one facility treated primarily municipal wastewater while the other only industrial wastewater. Real time PCR assays were developed to determine copy numbers for total 16S rDNA (a measure of biomass content), the amoA gene (a measure of ammonia-oxidizers), and the Nitrospira 16S rDNA gene (a measure of nitrite-oxidizers) in mixed liquor samples. In both the municipal and industrial WWTP samples, total 16S rDNA values were approximately 2-9 × 1013 copies/L and Nitrospira 16S rDNA values were 2-4 × 1010 copies/L. amoA gene concentrations averaged 1.73 × 109 copies/L (municipal) and 1.06 × 1010 copies/L (industrial), however, assays for two distinct ammonia oxidizing bacteria were required.

Challenges encountered when expanding activated sludge models: a case study based on N2O production

2014 ◽  
Vol 70 (7) ◽  
pp. 1251-1260 ◽  
Author(s):  
L. J. P. Snip ◽  
R. Boiocchi ◽  
X. Flores-Alsina ◽  
U. Jeppsson ◽  
K. V. Gernaey
Keyword(s):  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
N2o Emission ◽  
Full Scale ◽  
Ammonia Oxidizing Bacteria ◽  
N2o Formation ◽  
N2o Production ◽  
Parameter Values ◽  
Activated Sludge Models

It is common practice in wastewater engineering to extend standard activated sludge models (ASMs) with extra process equations derived from batch experiments. However, such experiments have often been performed under conditions different from the ones normally found in wastewater treatment plants (WWTPs). As a consequence, these experiments might not be representative for full-scale performance, and unexpected behaviour may be observed when simulating WWTP models using the derived process equations. In this paper we want to highlight problems encountered using a simplified case study: a modified version of the Activated Sludge Model No. 1 (ASM1) is upgraded with nitrous oxide (N2O) formation by ammonia-oxidizing bacteria. Four different model structures have been implemented in the Benchmark Simulation Model No. 1 (BSM1). The results of the investigations revealed two typical difficulties: problems related to the overall mathematical model structure and problems related to the published set of parameter values. The paper describes the model implementation incompatibilities, the variability in parameter values and the difficulties of reaching similar conditions when simulating a full-scale activated sludge plant. Finally, the simulation results show large differences in oxygen uptake rates, nitritation rates and consequently the quantity of N2O emission (GN2O) using the different models.

Respirometry for determination of the influents SS-concentration

1996 ◽  
Vol 33 (1) ◽  
pp. 311-323 ◽  
Author(s):  
A. Witteborg ◽  
A. van der Last ◽  
R. Hamming ◽  
I. Hemmers
Keyword(s):  
Experimental Design ◽  
Activated Sludge ◽  
Substrate Concentration ◽  
Full Scale ◽  
Activated Sludge Process ◽  
Respiration Rates ◽  
On Line ◽  
Large Measurement ◽  
Set Up

A method is presented for determining influent readily biodegradable substrate concentration (SS). The method is based on three different respiration rates, which can be measured with a continuous respiration meter which is operated in a cyclic way. Within the respiration meter nitrification is inhibited through the addition of ATU. Simulations were used to develop the respirometry set-up and decide upon the experimental design. The method was tested as part of a large measurement programme executed at a full-scale plant. The proposed respirometry set-up has been shown to be suitable for a semi-on-line determination of an influent SS which is fully based on the IAWQ #1 vision of the activated sludge process. The YH and the KS play a major role in the principle, and should be measured directly from the process.

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