Potensi Bahan Alami dalam Menekan Produksi CH4 dan N2O dari Tanah Sawah

Low nitrogen efficiency is one of the sources of greenhouse gas (GHG) emissions from rice fields. Methane (CH4) and nitrous oxide (N2O) emissions could be controlled by nitrification inhibitors (NI). However, NI that has been commercialized is expensive. Therefore, some natural materials should be developed as NI that is low cost, easy to use, low N2O and CH4, and eco-friendly. The objective of this study was to observe the effect of natural NI on the production potential of CH4 and N2O from paddy soil. The experiment in the laboratory was arranged in a factorial design (2 × 7 × 3 replication). The first factor was soil types (inceptisols and vertisols), and the second factor was natural NI (control, Cocos nucifera, Camellia sinensis, Coffea robusta, Curcuma domestica, Ageratum conyzoides). The results showed that the average CH4 production from the natural NI in the inceptisols and vertisols ranged 0,014-1,710 mg CH4 g soil-1 and 0,002-0,337 mg CH4 g soil-1, respectively. Application of natural NI reduced 32-69% CH4 production compare to control. Redox potential affected CH4 production. The chemical compound of the natural NI affected CH4 production in the soil. The application of coffee waste, coconut husk, tea waste, and Ageratum conyzoides reduced 60,71; 54,61; 64,83 dan 64,16% of N2O production in Inceptisols compare to control, respectively. Application of natural NI could contribute to save the environment because it decreased GHG production in paddy soil. Keywords: greenhouse gas, inceptisols, incubation experiment, natural nitrification inhibitors, vertisols

328 The Potential Role of Two Red Seaweeds That Promote Anti-methanogenic Activity and Rumen Fermentation Profiles Under Laboratory Conditions

Ruminal methane (CH4) production results from carbohydrate fermentation by ruminal microbiota (methanogens) to produce CH4, volatile fatty acids (VFA), carbon dioxide (CO2), nitrous oxide (N2O), and hydrogen (H2) in a reduction pathway. The aims of this study were to assess the effects of two red seaweed (RSW) species (Asparagopsis taxiformis and A. armata; collected from Messina, Italy). The two RSW were identified by DNA barcoding and genetic data were deposited in BOLD Systems (REAPP006-21, REAPP004-21, respectively). The two RSW were used at dietary inclusion levels (0, 2, and 4% as-fed basis) in an anaerobic in vitro study (39°C for 48-h) to examine greenhouse gas (GHG) production and VFA profiles. Gases were collected using an ANKOM Gas Production system and analyzed for CH4 and N2O by gas chromatography. Asparagopsis taxiformis contained higher levels of bromoform (201 vs. 7.0 mg/kg DM), iodine (4820 vs. 3260 ppm), and crude protein (16 vs. 15.6% DM), than A. armata, which contained higher levels of acid detergent fiber (ADF; 7.7 vs. 19.0%) and neutral detergent fiber (NDF; 13.2 vs. 19.2%), respectively. RSW supplementation increased total gas, butyrate and valerate production (P < 0.01), while production of CH4 (mg/g DM), acetate (A), propionate (P), A/P ratios and in vitro dry matter digestibility (IVDMD; % DM) were reduced (P < 0.01) as RSW supplementation increased. In the presence of A. taxiformis, production of N2O (µg/g DM), tended to be less (P = 0.1) at 2% DM, but increased (P < 0.01) N2O production with A. armata at the 2 and 4% DM. Therefore, it may be possible to suppress methanogenesis both directly and indirectly by addition of RSW. To efficiently use seaweeds as feed ingredients with nutritional and environmental benefits, more research is required to determine the mechanisms underlying seaweed and dietary substrate interactions.

Effects of Corn Stalks and Urea on N2O Production from Corn Field Soil

Returning corn stalks to the field is an important and widely used soil management practice which is conducive to the sustainable development of agriculture. In this study, the effects of corn stalks and urea on N2O production in corn field soil were investigated through a 21-day incubation experiment. This study showed that increasing amounts of urea added to soil with a history of corn cultivation leads to increasing overall N2O emissions, by increasing both the intensity and the duration of emissions. Although N2O production was affected primarily by urea-derived NH4+-N and NO3−-N, its main source was native soil nitrogen, which accounted for 78.5 to 94.5% of N2O. Returning corn stalk residue to the field reduced the production of N2O, and the more urea was applied, the stronger the effect of corn residue on reducing N2O emissions. Combining the application of corn stalks and urea could reduce the concentration of NH4+-N and NO3−-N derived from urea, and then reduce the substrate required for N2O production in nitrification and denitrification processes. In addition, the combined application of corn stalks and urea could effectively inhibit the abundance of key N2O-producing genes AOA amoA, nirS and nirK.

Microbial activity responses to water stress in agricultural soils from simple and complex crop rotations

Increasing climatic pressures such as drought and flooding challenge agricultural systems and their management globally. How agricultural soils respond to soil water extremes will influence biogeochemical cycles of carbon and nitrogen in these systems. We investigated the response of soils from long-term agricultural field sites under varying crop rotational complexity to either drought or flooding stress. Focusing on these contrasting stressors separately, we investigated soil heterotrophic respiration during single and repeated stress cycles in soils from four different sites along a precipitation gradient (Colorado, MAP 421 mm; South Dakota, MAP 580 mm; Michigan, MAP 893 mm; Maryland, MAP 1192 mm); each site had two crop rotational complexity treatments. At the driest (Colorado) and wettest (Maryland) of these sites, we also analyzed microbial biomass, six potential enzyme activities, and N2O production during and after individual and repeated stress cycles. In general, we found site specific responses to soil water extremes, irrespective of crop rotational complexity and precipitation history. Drought usually caused more severe changes in respiration rates and potential enzyme activities than flooding. All soils returned to control levels for most measured parameters as soon as soils returned to control water levels following drought or flood stress, suggesting that the investigated soils were highly resilient to the applied stresses. The lack of sustained responses following the removal of the stressors may be because they are well in the range of natural in situ soil water fluctuations at the investigated sites. Without the inclusion of plants in our experiment, we found that irrespective of crop rotation complexity, soil and microbial properties in the investigated agricultural soils were more resistant to flooding but highly resilient to drought and flooding during single or repeated stress pulses.