Characterization of multiple antibiotic resistance of culturable microorganisms and metagenomic analysis of total microbial diversity of marine fish sold in retail shops in Mumbai, India

2017 ◽  
Vol 25 (7) ◽  
pp. 6228-6239 ◽  
Author(s):  
Onkar A. Naik ◽  
Ravindranath Shashidhar ◽  
Devashish Rath ◽  
Jayant R. Bandekar ◽  
Archana Rath
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Diversity ◽  
Marine Fish ◽  
Metagenomic Analysis ◽  
Multiple Antibiotic Resistance

scholarly journals Incidence and Characterization of Integrons, Genetic Elements Mediating Multiple-Drug Resistance, in AvianEscherichia coli

1999 ◽  
Vol 43 (12) ◽  
pp. 2925-2929 ◽  
Author(s):  
Lydia Bass ◽  
Cynthia A. Liebert ◽  
Margie D. Lee ◽  
Anne O. Summers ◽  
David G. White ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Multiple Drug Resistance ◽  
Marker Gene ◽  
Clinical Isolates ◽  
Multiple Antibiotic Resistance ◽  
Class 1 Integron ◽  
E Coli ◽  
Class 1

ABSTRACT Antibiotic resistance among avian bacterial isolates is common and is of great concern to the poultry industry. Approximately 36% (n = 100) of avian, pathogenic Escherichia coli isolates obtained from diseased poultry exhibited multiple-antibiotic resistance to tetracycline, oxytetracycline, streptomycin, sulfonamides, and gentamicin. Clinical avian E. coli isolates were further screened for the presence of markers for class 1 integrons, the integron recombinase intI1 and the quaternary ammonium resistance gene qacEΔ1, in order to determine the contribution of integrons to the observed multiple-antibiotic resistance phenotypes. Sixty-three percent of the clinical isolates were positive for the class 1 integron markersintI1 and qacEΔ1. PCR analysis with the conserved class 1 integron primers yielded amplicons of approximately 1 kb from E. coli isolates positive for intI1 andqacEΔ1. These PCR amplicons contained the spectinomycin-streptomycin resistance gene aadA1. Further characterization of the identified integrons revealed that many were part of the transposon Tn21, a genetic element that encodes both antibiotic resistance and heavy-metal resistance to mercuric compounds. Fifty percent of the clinical isolates positive for the integron marker gene intI1 as well as for theqacEΔ1 and aadA1 cassettes also contained the mercury reductase gene merA. The correlation between the presence of the merA gene with that of the integrase and antibiotic resistance genes suggests that these integrons are located in Tn21. The presence of these elements among avianE. coli isolates of diverse genetic makeup as well as inSalmonella suggests the mobility of Tn21 among pathogens in humans as well as poultry.

scholarly journals Characterization of a multiple antibiotic resistance plasmid from Haemophilus ducreyi.

1989 ◽  
Vol 33 (9) ◽  
pp. 1627-1630 ◽  
Author(s):  
P J Willson ◽  
W L Albritton ◽  
L Slaney ◽  
J K Setlow
Keyword(s):  
Antibiotic Resistance ◽  
Haemophilus Ducreyi ◽  
Multiple Antibiotic Resistance ◽  
Resistance Plasmid

scholarly journals Prevalence and characterization of plasmid-mediated quinolone resistance in various aquatic sources

2021 ◽  
Author(s):  
Farhan Yusuf ◽  
Royce Ing
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Diversity ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Global Scale ◽  
Quinolone Resistance ◽  
Nutrient Loads

Ciprofloxacin is a fully synthetic antibiotic developed in 1987 and remains among the most widely used antibiotics worldwide Excessive usage has resulted in small quinolone concentrations found in aquatic sources Environments with subinhibitory antibiotic concentrations and high microbial diversity and nutrient loads (e g wastewater) foster the development of antibiotic resistance genes qnr genes conferring quinolone resistance were first found in 1998 and have since been observed on a global scale

scholarly journals Prevalence and characterization of plasmid-mediated quinolone resistance in various aquatic sources

2021 ◽  
Author(s):  
Farhan Yusuf ◽  
Royce Ing
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Diversity ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Global Scale ◽  
Quinolone Resistance ◽  
Nutrient Loads

Ciprofloxacin is a fully synthetic antibiotic developed in 1987 and remains among the most widely used antibiotics worldwide Excessive usage has resulted in small quinolone concentrations found in aquatic sources Environments with subinhibitory antibiotic concentrations and high microbial diversity and nutrient loads (e g wastewater) foster the development of antibiotic resistance genes qnr genes conferring quinolone resistance were first found in 1998 and have since been observed on a global scale

scholarly journals Characterization of MarR Superrepressor Mutants

1999 ◽  
Vol 181 (10) ◽  
pp. 3303-3306 ◽  
Author(s):  
Michael N. Alekshun ◽  
Stuart B. Levy
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Antibiotic Resistance ◽  
Dna Binding ◽  
Multiple Antibiotic Resistance ◽  
Mutant Proteins ◽  
Whole Cells

ABSTRACT MarR negatively regulates expression of the multiple antibiotic resistance (mar) locus in Escherichia coli. Superrepressor mutants, generated in order to study regions of MarR required for function, exhibited altered inducer recognition properties in whole cells and increased DNA binding to marO in vitro. Mutations occurred in three areas of the relatively small MarR protein (144 amino acids). It is surmised that superrepression results from increased DNA binding activities of these mutant proteins.

scholarly journals Isolation and Characterization of Listeria monocytogenes Isolates from Ready-To-Eat Foods in Florida

2006 ◽  
Vol 72 (7) ◽  
pp. 5073-5076 ◽  
Author(s):  
Yuelian Shen ◽  
Yan Liu ◽  
Yifan Zhang ◽  
Jennifer Cripe ◽  
William Conway ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Antibiotic Susceptibility ◽  
Food Samples ◽  
Multiple Antibiotic Resistance ◽  
Isolation And Characterization ◽  
Lineage 2 ◽  
Conventional Methods ◽  
Specific Bacteriophage

ABSTRACT Of 3,063 ready-to-eat food samples tested, 91 (2.97%) were positive for Listeria monocytogenes, and lineage 1 strains outnumbered lineage 2 strains 57 to 34. Seventy-one isolates (78%) exhibited multiple antibiotic resistance, and an L. monocytogenes-specific bacteriophage cocktail lysed 65 of 91 (71%) isolates. Determining phage, acid, and antibiotic susceptibility phenotypes enabled us to identify differences among strains which were otherwise indistinguishable by conventional methods.

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