Protein adsorption behaviors on chitosan/poly(ɛ-caprolactone) blend films studied by quartz crystal microbalance with dissipation monitoring (QCM-D)

2009 ◽  
Vol 52 (8) ◽  
pp. 2275-2279 ◽  
Author(s):  
Rong Zeng ◽  
Yi Zhang ◽  
ZhiHong Liang ◽  
Mei Tu ◽  
ChangRen Zhou
Keyword(s):  
Protein Adsorption ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Blend Films ◽  
Adsorption Behaviors

Protein Adsorption on Hydroxyapatite Nano-Crystals with Quartz Crystal Microbalance Technique

2007 ◽  
Vol 361-363 ◽  
pp. 1119-1122 ◽  
Author(s):  
Tomohiko Yoshioka ◽  
Toshiyuki Ikoma ◽  
Akira Monkawa ◽  
Toru Tonegawa ◽  
Dinko Chakarov ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Quartz Crystal Microbalance ◽  
Conformational Changes ◽  
Quartz Crystal ◽  
Phase Changes ◽  
Basic Proteins ◽  
Adsorption Behaviors ◽  
Acidic Proteins ◽  
Straight Lines ◽  
Constant Slope

Real time adsorption behaviors of six proteins with different isoelectric points on hydroxyapatite (HAp) nanocrystal surfaces have been investigated by using HAp sensors for quartz crystal microbalance with dissipation technique (QCM-D). The dissipation (D)–frequency (f) plots clearly showed that the different types of protein adsorption behaviors; the D-f plots of acidic proteins lie on one straight line with a constant slope under all initial protein concentrations, while those of neutral and basic proteins lie on two straight lines with different slopes. The acidic proteins formed a monolayer, while the neutral and basic proteins could cause conformational changes with the adsorbed amount of proteins. The QCM-D technique with novel HAp nanocrystal sensor is useful for the liquid phase changes of proteins on the surface.

scholarly journals Protein adsorption on and swelling of polyelectrolyte brushes: A simultaneous ellipsometry-quartz crystal microbalance study

2010 ◽  
Vol 5 (4) ◽  
pp. 159-167 ◽  
Author(s):  
Eva Bittrich ◽  
Keith Brian Rodenhausen ◽  
Klaus-Jochen Eichhorn ◽  
Tino Hofmann ◽  
Mathias Schubert ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Polyelectrolyte Brushes

scholarly journals Protein Adsorption to Titanium and Zirconia Using a Quartz Crystal Microbalance Method

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
You Kusakawa ◽  
Eiji Yoshida ◽  
Tohru Hayakawa
Keyword(s):  
Surface Roughness ◽  
Protein Adsorption ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Adsorption Rate ◽  
Force Microscopy ◽  
Atomic Force ◽  
A Cell ◽  
Addition Amount

Protein adsorption onto titanium (Ti) or zirconia (ZrO2) was evaluated using a 27 MHz quartz crystal microbalance (QCM). As proteins, fibronectin (Fn), a cell adhesive protein, and albumin (Alb), a cell adhesion-inhibiting protein, were evaluated. The Ti and ZrO2 sensors for QCM were characterized by atomic force microscopy and electron probe microanalysis observation, measurement of contact angle against water, and surface roughness. The amounts of Fn and Alb adsorbed onto the Ti and ZrO2 sensors and apparent reaction rate were obtained using QCM measurements. Ti sensor showed greater adsorption of Fn and Alb than the ZrO2 sensor. In addition, amount of Fn adsorbed onto the Ti or ZrO2 sensors was higher than that of Alb. The surface roughness and hydrophilicity of Ti or ZrO2 may influence the adsorption of Fn or Alb. With regard to the adsorption rate, Alb adsorbed more rapidly than Fn onto Ti. Comparing Ti and ZrO2, Alb adsorption rate to Ti was faster than that to ZrO2. Fn adsorption will be effective for cell activities, but Alb adsorption will not. QCM method could simulate in vivo Fn and Alb adsorption to Ti or ZrO2.

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