Transcriptional expression profile of cultured human embryonic stem cells in vitro and in vivo

2012 ◽  
Vol 48 (3) ◽  
pp. 165-174 ◽  
Author(s):  
Marlen Keil ◽  
Antje Siegert ◽  
Klaus Eckert ◽  
Jörg Gerlach ◽  
Wolfram Haider ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Expression Profile ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Transcriptional Expression

In Vitro Differentiation and In Vivo Mineralization of Osteogenic Cells Derived from Human Embryonic Stem Cells

2004 ◽  
Vol 10 (9-10) ◽  
pp. 1518-1525 ◽  
Author(s):  
Robert C. Bielby ◽  
Aldo R. Boccaccini ◽  
Julia M. Polak ◽  
Lee D.K. Buttery
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
In Vitro Differentiation ◽  
Osteogenic Cells

scholarly journals A protocol for the differentiation of human embryonic stem cells into dopaminergic neurons using only chemically defined human additives: Studies in vitro and in vivo

2007 ◽  
Vol 1127 ◽  
pp. 19-25 ◽  
Author(s):  
Lorraine Iacovitti ◽  
Angela E. Donaldson ◽  
Cheryl E. Marshall ◽  
Sokreine Suon ◽  
Ming Yang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Dopaminergic Neurons ◽  
Embryonic Stem

Generation of T Cells from Human Embryonic Stem Cells.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 1527-1527
Author(s):  
Frank Timmermans ◽  
Imke Velghe ◽  
Lieve Van Walleghem ◽  
Magda De Smedt ◽  
Stefanie Van Coppernolle ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Early Stage ◽  
Embryonic Stem ◽  
Hesc Line ◽  
Double Positive

Abstract Background: Human embryonic stem cells (hESC) are derived from early stage blastocysts and are characterized by the ability to both self-renew and to generate differentiated functional cell types. One of the major challenges in the field of hESC research, is to set up a culture system that drives hESC down a particular lineage fate. To date, studies reporting hematopoietic development have not provided evidence on the differentiation capacity of hESC into T lineage cells in vitro. Material and Methods: hESC line H1 (National Institutes of Health [NIH] code: WA01), Wisconson, Madison, USA) was used (Passage 30–60) in all experiments. The hESC line was kept in an undifferentiated state on MEFs as previously described. OP9 cells and OP9 cells that express high levels of the Notch ligand Delta-like 1 (OP9-DLL1, a gift from J. C. Zuniga-Pflücker, University of Toronto, Canada) were cultured as previously described in MEM-α with 20 % FCS. Results: Our data show that T cells can be generated in vitro from hESC in a robust and highly reproducible manner using the sequential exposure of hESC to the murine OP9 cell line and OP9-DLL1. On OP9 stromal layers, a CD34highCD43dim hematopoietic precursor population is generated that is confined to vascular-like structures, reminiscent of blood islands that emerge during in vivo embryonic development. This precursor population becomes T lineage committed when exposed to OP9-DLL1 monolayers, passing sequentially through a CD34+CD7+ phenotype, a CD4+CD8+ double positive intermediate stage and eventually differentiates into a mature T cells. Polyclonal T cells are generated, cell receptor (TCR) alpha-beta and TCRgamma-delta which are functional based on proliferative capacity and production of cytokines after TCR crosslinking. Conclusion: We show that mature and functional T cells can be generated from hESC using well defined in vitro conditions. This protocol in combination with the recently described induced pluripotent cells may find clinical applicability in tumor immunology.

scholarly journals Immunomodulative effects of mesenchymal stem cells derived from human embryonic stem cells in vivo and in vitro

2011 ◽  
Vol 12 (1) ◽  
pp. 18-27 ◽  
Author(s):  
Zhou Tan ◽  
Zhong-yuan Su ◽  
Rong-rong Wu ◽  
Bin Gu ◽  
Yu-kan Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem

scholarly journals In Vitro- and In Vivo-Induced Transgene Expression in Human Embryonic Stem Cells and Derivatives

Stem Cells ◽  
2008 ◽  
Vol 26 (2) ◽  
pp. 525-533 ◽  
Author(s):  
Xiaofeng Xia ◽  
Melvin Ayala ◽  
Benjamin R. Thiede ◽  
Su-Chun Zhang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Transgene Expression ◽  
Embryonic Stem

scholarly journals Directed In Vitro Myogenesis of Human Embryonic Stem Cells and Their In Vivo Engraftment

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e72023 ◽  
Author(s):  
Yongsung Hwang ◽  
Samuel Suk ◽  
Susan Lin ◽  
Matthew Tierney ◽  
Bin Du ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem
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