Isolation, characterization, and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line

2012 ◽  
Vol 49 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Ramakant Kaushik ◽  
Karn Pratap Singh ◽  
Archana Kumari ◽  
Manoj Kumar Singh ◽  
Radhey Shyam Manik ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Cell Line ◽  
Bubalus Bubalis ◽  
Egfp Expression ◽  
Epithelial Cell Line ◽  
Mammary Gland Epithelial Cell

Cell-Cell Contact Down-Regulates Expression of Membrane Type MetaIIoproteinase-1 (MT1-MMP) in a Mouse Mammary Gland Epithelial Cell Line

1997 ◽  
Vol 14 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Satomi S. Tanaka ◽  
Yukiyasu Mariko ◽  
Hidetoshi Mori ◽  
Junko Ishijima ◽  
Sumie Tachi ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Cell Line ◽  
Mouse Mammary Gland ◽  
Epithelial Cell Line ◽  
Cell Contact ◽  
Mammary Gland Epithelial Cell ◽  
Membrane Type ◽  
Cell Cell

Demonstration of Type II Hemidesmosomes in a Mammary Gland Epithelial Cell Line, BMGE-H1

1994 ◽  
Vol 115 (3) ◽  
pp. 469-476 ◽  
Author(s):  
Jun Uematsu ◽  
Yuji Nishizawa ◽  
Arnoud Sonnenberg ◽  
Katsushi Owaribe
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Type Ii ◽  
Mammary Gland Epithelial Cell

scholarly journals Establishment and Characterization of a Buffalo (Bubalus bubalis) Mammary Epithelial Cell Line

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e40469 ◽  
Author(s):  
Vijay Anand ◽  
Nilambra Dogra ◽  
Surender Singh ◽  
Sudarshan N. Kumar ◽  
Manoj K. Jena ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Mammary Epithelial Cell ◽  
Bubalus Bubalis ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line

scholarly journals Establishment of Mammary Gland Model In Vitro: Culture and Evaluation of a Yak Mammary Epithelial Cell Line

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e113669 ◽  
Author(s):  
Mei Fu ◽  
Yabing Chen ◽  
Xianrong Xiong ◽  
Daoliang Lan ◽  
Jian Li
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
In Vitro Culture ◽  
Cell Line ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line

scholarly journals Establishment and Characterization of a Lactating Caprine Mammary Gland Luminal Epithelial Cell Line

2019 ◽  
Vol 11 (4) ◽  
pp. 36
Author(s):  
Mahipal Singh ◽  
Benjamin Hortman ◽  
Venkata Degala ◽  
Xiaoling Ma
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Line ◽  
Milk Production ◽  
Cell Cultures ◽  
Epithelial Cell Line ◽  
Luminal Epithelial Cell ◽  
Saanen Goat

Mammary gland is a defining characteristic of mammalian species which produces nutritious milk and plays a major role in the development of newborns. The gland contains a series of ducts and crevices leading back to alveoli, which contain milk producing cells called luminal epithelial cells. These cells, if cultured in-vitro, can be utilized to explore the metabolic processes occurring during milk production. The knowledge thus gained can be used to manipulate the system to enhance milk production and/or modify its composition. The main objective of this study was to establish a luminal epithelial cell-line from a lactating goat. Explant culture technique was used to produce primary cells from the mammary tissue of a 4-year-old lactating Saanen goat. The outgrowing cells were purified by selective trypsinization to remove fibroblast cells in 3-4 serial passages. The purified cell cultures exhibited cobblestone morphology, typical of the mammary epithelial cells, formed clear islands when plated in low density, and exhibited dome-shaped structures, if cultured for extended time. The cells stained positive with anti-human cytokeratin 18 antibodies, confirming their epithelial nature. Cell cultures also stained positive with rabbit anti-bovine β-lactoglobulin antibodies, indicating milk production in these cells. The cell-line has potential as an in-vitro cell model to understand signaling during milk synthesis, mammary gland development, and testing DNA constructs for therapeutic protein secretion in milk, prior to production of transgenic goats.

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