Vascular endothelial growth factor ameliorated palmitate-induced cardiomyocyte injury via JNK pathway

AbstractEnhanced apoptosis of cardiomyocytes in suffering overloaded saturated fatty acids (SFAs) can result in myocardial infarction and cardiac dysfunction. The function of vascular endothelial growth factor (VEGF) in cardiomyocyte protection was not clearly described. To investigate the preservative effects of VEGF sensitization on ceramide-mediated programmed cell death of cardiomyocytes, palmitate-induced injury in H9c2 cells was established as an in vitro model. Results revealed that 0.5 mM palmitate application effectively led to debased viability and activated apoptotic factors. A significant time-dependent relation between PAL and cardiomyocyte injury was observed. The apoptosis rate was increased greatly after 16 h of treatment with 0.5 mM PAL. In addition, cell viability was restored by VEGF overexpression during treatment with 0.5 mM PAL. Reduced apoptosis rate and expression of caspase 3, Bax, and NF-κB p65 were observed in this process, while boosted Bcl-2, p-JNK/JNK expression and activity of caspase 3 were checked. However, p-ERK/ERK levels did not exhibit a significant change. These findings indicated the protective effects of VEGF in confronting the ceramide-induced cardiomyocyte apoptosis, and would devote therapeutic targets for cardiovascular safeguard in dealing with fatty acid stress.

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Vascular endothelial growth factor but not placental growth factor promotes trophoblast syncytialization in vitro

Journal of the Society for Gynecologic Investigation ◽

10.1016/s1071-5576(01)00134-4 ◽

2001 ◽

Vol 8 (6) ◽

pp. 341-346 ◽

Cited By ~ 20

Author(s):

I Crocker

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Placental Growth Factor ◽

Vascular Endothelial ◽

Endothelial Growth Factor

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Regulation of vascular endothelial growth factor expression in human endometrium by steroids and hypoxia: In vitro and in vivo studies

Journal of the Society for Gynecologic Investigation ◽

10.1016/s1071-5576(97)86195-3 ◽

1998 ◽

Vol 5 (1) ◽

pp. 69A-69A

Author(s):

A SHARKEY ◽

D CHARNOCKJONES ◽

K DAY ◽

H SOWTER ◽

L SVENSSON ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Human Endometrium ◽

In Vivo Studies ◽

Vascular Endothelial ◽

Factor Expression ◽

Growth Factor Expression ◽

Endothelial Growth Factor

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Heparin‐modified alginate microspheres enhance neovessel formation in hiPSC ‐derived endothelial cells and heterocellular in vitro models by controlled release of vascular endothelial growth factor

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.37168 ◽

2021 ◽

Author(s):

Fabiola Munarin ◽

Carly Kabelac ◽

Kareen L.K. Coulombe

Keyword(s):

Vascular Endothelial Growth Factor ◽

Endothelial Cells ◽

Controlled Release ◽

Growth Factor ◽

In Vitro Models ◽

Vascular Endothelial ◽

Endothelial Growth Factor

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In vitro effects of dexamethasone on hypoxia-induced hyperpermeability and expression of vascular endothelial growth factor

European Journal of Pharmacology ◽

10.1016/s0014-2999(00)00915-8 ◽

2001 ◽

Vol 411 (3) ◽

pp. 231-243 ◽

Cited By ~ 84

Author(s):

Silvia Fischer ◽

Dieter Renz ◽

Wolfgang Schaper ◽

Gerhard F. Karliczek

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Vascular Endothelial ◽

In Vitro Effects ◽

Endothelial Growth Factor

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Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro

Histochemistry and Cell Biology ◽

10.1007/s00418-021-02016-6 ◽

2021 ◽

Author(s):

Kamil Wartalski ◽

Gabriela Gorczyca ◽

Jerzy Wiater ◽

Zbigniew Tabarowski ◽

Małgorzata Duda

Keyword(s):

Stem Cells ◽

Vascular Endothelial Growth Factor ◽

Endothelial Cells ◽

Growth Factor ◽

Primary Component ◽

Marker Genes ◽

Vascular Endothelial ◽

Ovarian Cortex ◽

Endothelial Growth Factor

AbstractEndothelial cells (ECs), the primary component of the vasculature, play a crucial role in neovascularization. However, the number of endogenous ECs is inadequate for both experimental purposes and clinical applications. Porcine ovarian putative stem cells (poPSCs), although not pluripotent, are characterized by great plasticity. Therefore, this study aimed to investigate whether poPSCs have the potential to differentiate into cells of endothelial lineage. poPSCs were immunomagnetically isolated from postnatal pig ovaries based on the presence of SSEA-4 protein. Expression of mesenchymal stem cells (MSCs) markers after pre-culture, both at the level of mRNA: ITGB1, THY, and ENG and corresponding protein: CD29, CD90, and CD105 were significantly higher compared to the control ovarian cortex cells. To differentiate poPSCs into ECs, inducing medium containing vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF), epidermal growth factor (EGF), ascorbic acid, and heparin was applied. After 14 days, poPSC differentiation into ECs was confirmed by immunofluorescence staining for vascular endothelial cadherin (VECad) and vascular endothelial growth factor receptor-2 (VEGFR-2). Semi-quantitative WB analysis of these proteins confirmed their high abundance. Additionally, qRT-PCR showed that mRNA expression of corresponding marker genes: CDH5, KDR was significantly higher compared with undifferentiated poPSCs. Finally, EC functional status was confirmed by the migration test that revealed that they were capable of positive chemotaxis, while tube formation assay demonstrated their ability to develop capillary networks. In conclusion, our results provided evidence that poPSCs may constitute the MSC population in the ovary and confirmed that they might be a potential source of ECs for tissue engineering.

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