The growth regulators used for bud regeneration and shoot rooting affect the competence for flowering and seed set in regenerated plants of protein peas

2000 ◽  
Vol 36 (3) ◽  
pp. 188-193 ◽  
Author(s):  
S. J. Ochatt ◽  
C. Pontécaille ◽  
M. Rancillac
Keyword(s):  
Growth Regulators ◽  
Seed Set ◽  
Bud Regeneration ◽  
Regenerated Plants

scholarly journals INFLUENCE OF GROWTH REGULATORS ON OVULES ABORTION, SEED SET AND ITS GERMENATION OF TWO ALFALFA CULTIVARS

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
Khrbeet & Al-Beiruty
Keyword(s):  
Growth Regulators ◽  
Seed Set ◽  
Foliar Application ◽  
Control Treatment ◽  
Naphthalene Acetic Acid ◽  
Flower Buds ◽  
College Of Agriculture ◽  
Vegetation Growth ◽  
Main Plot ◽  
And Control

A field experiment was conducted at the experimental farm, College of Agriculture, Abu-Ghraib, Baghdad, Iraq during the period from mid. of March 2012 to mid. of July 2014 to investigate the effects of plant growth regulators and foliar application stages on ovules abortion, seed set and its germination of two alfalfa cultivars. Layout of the experiment was R.C.B.D. arranged in split-split plot with three replications. Cultivars (local and hamedan) were assigned as a main plot and foliar application stages (vegetative growth, flower buds emergence, 50% flowering and 80% flowering) were assigned as sub-plots, while growth regulators (Alar, Ethephon, Naphthalene acetic acid(NAA) and control treatment) were assigned in the sub-sub-plots. Result showed that there were no significant differences between cultivars in all traits. Foliar application of NAA increased No. of ovules per floret (9.11) compare with other treatments, but it was not significantly different compare with Alar. In 1st and 2nd seed crop, plants sprayed with Alar at flower buds emergence stage gave highest seed set (5.17, 6.93) respectively. Percentage of ovules abortion significantly influenced by growth regulaters, folair application stages and their interaction. Since, foliar application of Alar at beginning of flower buds emergence reduce % of ovules abortion (30.89). In general, plants sprayed at vegetation growth stage increased % of seed germination. It was clear from this study that more than 40% of ovules were aborted, this may be one of the reasons for reduction of seed yield in alfalfa, therefore, we recommended to do more researches in this field in order to know the causes of ovules abortion.

REGENERATION OF TREMA ORIENTALIS (BLUME) LINN.: EFFECT OF GROWTH REGULATORS, CULTURE CONDITIONS, AND AGE AND SOURCE OF EXPLANTS

1995 ◽  
Vol 43 (4) ◽  
pp. 391-395 ◽  
Author(s):  
G.R. Rout ◽  
S. Samantaray ◽  
P. Das
Keyword(s):  
Growth Regulators ◽  
Leaf Explants ◽  
Naphthaleneacetic Acid ◽  
Mature Trees ◽  
Regeneration Rate ◽  
Bud Regeneration ◽  
Shoot Bud ◽  
Shoot Bud Regeneration ◽  
Trema Orientalis

Optimal conditions for high frequency shoot bud regeneration from leaf callus of Trema orientalis (Blume) Linn. were studied. The regeneration rate was controlled by the growth regulators, the age and the source of the explants, and the illumination conditions. Irrespective of illumination conditions, shoot bud regeneration was achieved only in media containing benzyladenine (BA) + α-naphthaleneacetic acid (NAA) combinations, with the best results being obtained in the presence of 2.5 mg/1 BA and 0.25–0.5 mg/1 NAA. The morphogenic response was less frequent in the calluses derived from leaf explants of the mature trees compared to those of the in vitro-grown seedlings. The rate of shoot bud regeneration was more pronounced in the cultures maintained for 4 weeks in the light (16-h photoperiod) than the cultures incubated in the dark. Regenerated shoots were rooted on the medium containing 1/2 strength basal Murashige and Skoog (MS) salts supplemented with 0.01 mg/1 NAA or indole-3-butyric acid (IBA). The rooted plantlets were established in the greenhouse.

Chromosome 5D instability in cell lines of Triticum tauschii and morphological variation in regenerated plants

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 737-742 ◽  
Author(s):  
M. O. Winfield ◽  
A. Karp ◽  
P. A. Lazzeri ◽  
M. R. Davey
Keyword(s):  
Seed Set ◽  
Immature Embryo ◽  
Cell Suspensions ◽  
Chromosome Counts ◽  
Triticum Tauschii ◽  
Regenerated Plants ◽  
Immature Embryo Culture ◽  
Meiotic Analyses ◽  
L2 Medium ◽  
Indole 3 Acetic Acid

Immature embryos of Triticum tauschii cultured on L2 medium with 30 g∙L−1maltose gave rise to compact and highly structured callus that readily regenerated shoots when transferred to MS medium supplemented with zeatin and indole-3-acetic acid. Two cell suspensions were initiated from the callus induced on L2 medium. An analysis of chromosome number in these cell suspensions after 3 months of culture showed that 95 and 75% of the cells had a normal complement. After 5 months, in both lines the majority of cells had chromosome complements of 2n = 13 and many of these aneuploid cells possessed a dicentric chromosome. C-banding indicated that the dicentric was often formed from chromosomes 2D and 5D. Fifty-six plants regenerated from callus were grown to maturity. Variation was observed in tiller number, flowering time, and seed-set; in two cases, the variation in early flowering was shown to be heritable. All the regenerants analysed had normal chromosome counts (2n = 2x = 14) and meiotic analyses of 35 plants revealed no obvious structural rearrangements.Key words: immature embryo culture, regeneration, chromosome, cell suspension, Triticum tauschii.

scholarly journals In vitro Mass Propagation from Shoot Tip of Dendrobium Red Bull - An Endangered Epiphytic Orchid

2018 ◽  
Vol 28 (2) ◽  
pp. 161-169 ◽  
Author(s):  
AA Mamun ◽  
MM Islam ◽  
M Ahmed ◽  
GC Ghosh
Keyword(s):  
Growth Regulators ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Good Growth ◽  
Multiple Shoot Induction ◽  
Best Response ◽  
Regenerated Plants ◽  
Shoot Number ◽  
Red Bull

An experiment was carried out to optimize the doses and combinations of growth regulators for in vitro propagation of Dendrobium Red Bull through shoot tip culture. The experiment was laid out according to completely randomized design with 15 treatments and three replications. For multiple shoot induction, newly grown shoot buds (keikies) were cultured on MS supplemented with various combinations of NAA and BAP. The regenerated plants were transferred to ex vitro in different substrates and acclimatized. Best response for the shoot length (21.19 mm) was obtained from the medium supplemented with 3.0 mg/l BAP with 1.5 mg/l NAA. Similarly maximum number of leaves was observed from the same combination at all the recorded days except 60 days, whereas the maximum shoot number (7.66) was obtained from the medium containing 3.0 mg/l BAP + 1.0 mg/l NAA. The shoots were then transferred to root inducing medium and well rooted plants were transferred to different substrates. Survivability of the regenerated plants varied from 60 to 92%. The substrate containing cocodust showed maximum survivability (92%) whereas minimum (60%) was observed from the substrate containing course sand. BAP and NAA proved to be good growth regulators for in vitro multiplication of Dendrobium. Plant Tissue Cult. & Biotech. 28(2): 161-169, 2018 (December)

scholarly journals Velvetleaf regenerates and reproduces after cropping

2018 ◽  
Vol 71 ◽  
pp. 349
Author(s):  
Claire A. Dowsett ◽  
Trevor K. James
Keyword(s):  
Seed Set ◽  
Management Strategies ◽  
Late Summer ◽  
Ground Level ◽  
Abutilon Theophrasti ◽  
Viable Seed ◽  
Maize Silage ◽  
Above Ground Level ◽  
Regenerated Plants ◽  
Cutting Height

Velvetleaf (Abutilon theophrasti) was accidentally introduced into New Zealand in 2016 and intensive efforts are being implemented to mitigate the risk of its naturalisation. This study investigated the possibility of velvetleaf plants surviving in maize fields following silage harvest in late summer and early autumn. Velvetleaf plants were grown to maturity in a glasshouse during the summer of 2017—2018. They were left to seed and senesce naturally and, on 28 February 2018, five senesced plants were “harvested” at each height (0, 30, 50, 70 and 150 mm) with varying numbers of leaf nodes remaining (0, 0, 1, 2 and 3 pairs respectively). Velvetleaf was capable of regeneration 2—4 weeks after “harvest” from these leaf nodes. Forty percent regeneration occurred with 1 pair of nodes; 80% (2 pairs); and 100% (3 pairs). All regenerated plants subsequently produced mature seed pods with viable seed (95% germination) 6—10 weeks following “harvest”. Maize silage crops are normally cut ~100 mm above ground level. In this study, velvetleaf was able to regenerate and reproduce following simulated maize silage harvest at, below and above, normal cutting height. Growers are advised to continue management strategies for velvetleaf to prevent seed set following maize silage harvest and before sowing of the next crop or pasture.

Reducing height and lodging in rapeseed with growth regulators

1991 ◽  
Vol 31 (2) ◽  
pp. 245 ◽  
Author(s):  
EL Armstrong ◽  
HI Nicol
Keyword(s):  
Growth Regulators ◽  
Seed Set ◽  
New South ◽  
Commercial Development ◽  
Chlormequat Chloride ◽  
Stem Strength ◽  
South Wales ◽  
Measured Effect ◽  
Yield Responses ◽  
Seed Yields

The plant growth regulators (PGRs) ethrel (20% a.i. 2-chloroethane phosphonic acid), PP 333 (25% w/v a.i. paclobutrazol), cycocel (50% w/v a.i. chlormequat chloride), pix (5% w/v a.i. mepiquat chloride) and EL 500 (50% w/w a.i. flurprimidol) were applied to bulky crops of Jumbuck, Marnoo and RU 14 rapeseed at Cowra and Woodstock in central-western New South Wales, in an effort to reduce their height and lodging. The most effective growth retardants under evaluation were PP 333 and EL 500, and this was achieved without apparent phytotoxicity. Height reductions were enhanced as rates were increased from 0 to 2 Lha, and the heights of Jumbuck, Mamoo and RU 14 were reduced on average by 22% (from 159 to 124 cm), 26% (from 136 to 100 cm) and 14% (from 150 to 129 cm), respectively. Significant reductions in lodging accompanied these changes. Ethrel resulted in some phytotoxicity, while pix and cycocel had little or no measured effect on any plant characters. Jumbuck was the tallest variety and the most severely lodged, and under these conditions, yield responses to PGR were large (900 kg/ha or 54%). Shorter, erect plants produced an even, compact pod canopy, and as a result, ripening was more uniform, pod shattering reduced and harvesting more efficient. In situations where seed yields were increased, these practical considerations, rather than physiological responses (e.g. pod and seed set), were largely responsible. These findings support further research and commercial development of PGRs for use in canola production in the more favoured areas and support the inclusion of reduced plant height and increased stem strength as integral components of the overall breeding objectives for canola in Australia.

scholarly journals Broccoli various genotypes regenerated plants (R0) seed set after geitonogamy

2020 ◽  
pp. 43-46
Author(s):  
E. A. Zablotskaya ◽  
A. I. Mineykina ◽  
E. A. Domblides ◽  
T. O. Paslova ◽  
L. L. Bondareva
Keyword(s):  
Seed Set ◽  
Regenerated Plants

scholarly journals Micropropagation of Juvenile and Adult Flowering Ash

1992 ◽  
Vol 117 (2) ◽  
pp. 346-350 ◽  
Author(s):  
Isabel Arrillaga ◽  
Victoria Lerma ◽  
Juan Segura
Keyword(s):  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Nodal Segments ◽  
Induction Medium ◽  
Naphthaleneacetic Acid ◽  
Shoot Apices ◽  
Regenerated Plants ◽  
Adult Trees

A protocol for in vitro propagation in flowering ash (Fraxinus ornus L.) has been developed. Shoot apices or nodal segments from aseptically grown seedings or shoot apices from adult trees were used as initial explants. Highest shoot multiplication rates were obtained when the explants were cultured for 30 days in liquid Rugini induction medium supplemented with BA followed by 30 days on solidified Rugini multiplication medium without growth regulators. Regenerated shoots were rooted on Heller medium containing auxins alone or in combination with BA. Rooting percentages up to 71% (juvenile material) or 50% (adult material) were obtained in the presence of NAA and BA, and were not improved by treating the basal end of the shoots with concentrated NAA solutions. Following conventional procedures, regenerated plants were transferred to soil with more than 80% success. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); 1-naphthaleneacetic acid (NAA).

scholarly journals Micropropagation and assessment of somaclonal variation in Galanthus transcaucasicus in vitro plantlets

2021 ◽  
Vol 27 (4) ◽  
pp. 505-515
Author(s):  
Narges Asadi ◽  
Hossein Zarei ◽  
Seyyed Hamidreza Hashemi-Petroudi ◽  
Seyyed Javad Mousavizadeh
Keyword(s):  
Genetic Variation ◽  
Plant Growth ◽  
In Vitro Culture ◽  
Somaclonal Variation ◽  
Growth Regulators ◽  
Naphthaleneacetic Acid ◽  
In Vitro Plantlets ◽  
Regenerated Plants ◽  
High Concentrations

Abstract In vitro culture of twin-scaling explants of Galanthus transcaucasicus with different concentrations of plant growth regulators (PGRs) including 0.5, 1, 2, 3, 4, 6, 8, and 10 mg L-1 naphthaleneacetic acid (NAA) and 0.5, 1, 2, 3, and 4 mg L-1 benzyladenine (BA) was studied. After 18 weeks, the number of regenerated bulblets and intensity of callus was measured. Subsequently, bulblets were transferred to a medium with 0.5, 1, 2, 3, and 4 mg L-1 NAA and 0.5, 1, 2, 3, and 4 mg L-1 BA and, after 15 weeks, the bulblets length and diameter were measured. The highest intensity of callus was obtained on 4 mg L-1 NAA or 8 mg L-1 NAA with 1 mg L-1 BA. The highest number of regenerated bulblets was detected with 6 mg L-1 NAA and 2 mg L-1 BA. The highest diameter of bulblets occurred on four mgL-1 NAA (9.4 mm), while the lowest was observed on 0.5 mg L-1 BA (1.83 mm). The analysis of genetic variation using ISSR revealed that there was no somaclonal variation among the regenerated plants from BA and low level of NAA, but there was a significant somaclonal variation at high concentrations of NAA.

Sign in / Sign up

Export Citation Format

Share Document