An efficient procedure for embryogenic callus induction and double haploid plant regeneration through anther culture of Thai aromatic rice (Oryza sativa L. subsp. indica)

2009 ◽  
Vol 45 (2) ◽  
pp. 171-179 ◽  
Author(s):  
Suriyan Cha-um ◽  
Bootsaya Srianan ◽  
Aussanee Pichakum ◽  
Chalermpol Kirdmanee
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Double Haploid ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Haploid Plant ◽  
Aromatic Rice ◽  
Efficient Procedure ◽  
Embryogenic Callus Induction

Effect of 2,4-D on Embryogenic Callus Induction of Malaysian indica Rice (Oryza sativa L.) Cultivars MR123 and MR127

2013 ◽  
Vol 64 (2) ◽  
Author(s):  
Fauziah Illyas Ahmad ◽  
Nur Shafiqoh Johan ◽  
Alina Wagiran
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Staining Method ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Simple Protocol ◽  
Basal Media ◽  
Embryogenic Callus Induction ◽  
Mature Seeds

The aim of this study is to study the effect of various concentrations of 2,4-D on embryogenic callus induction of indica rice MR123 cultivar and MR127 cultivar using mature seeds. Optimal media for induction of callus of both cultivars was MS basal media supplemented with 30g/L sucrose, 500mg/L glutamine and 500mg/L proline supplemented with 2.5 mg/L 2,4-D. The highest percentage of callus induction was 70% and 76% for MR123 and MR127 respectively. Both cultivars produced an embryogenic callus from scutellum after a week in culture with white-yellowish in color. The viability tested using Evans blue show callus obtained was embryogenic. This simple protocol using staining method could be used for screening embryogenic callus before further experiments can be conducted.

scholarly journals High frequency plant regeneration from embryogenic callus of a popular indica rice (Oryza sativa L.)

2009 ◽  
Vol 15 (4) ◽  
pp. 371-375 ◽  
Author(s):  
Alagarsamy Karthikeyan ◽  
Shunmugiah Thevar Karutha Pandian ◽  
Manikandan Ramesh
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
High Frequency ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice

scholarly journals Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang)

2017 ◽  
Vol 18 (2) ◽  
pp. 91
Author(s):  
Kunti Anis Azizah ◽  
Didik Pudji Restanto ◽  
Bambang Sugiharto
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Rice Variety ◽  
Rice Cultivar ◽  
Medium Composition ◽  
Regeneration Frequency ◽  
Optimum Result

Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization. Keywords: Somatic embryo, Indica rice (Oryza sativa L. var. Ciherang), Callus

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