Stress treatments influence efficiency of microspore embryogenesis and green plant regeneration in hexaploid triticale (× Triticosecale Wittmack L.)

2013 ◽  
Vol 50 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Tobias Würschum ◽  
Matthew R. Tucker ◽  
Hans Peter Maurer
Keyword(s):  
Plant Regeneration ◽  
Microspore Embryogenesis ◽  
Green Plant ◽  
Hexaploid Triticale ◽  
Triticosecale Wittmack

scholarly journals Impact of Ionic Liquids on Induction of Wheat Microspore Embryogenesis and Plant Regeneration

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 839
Author(s):  
Dorota Weigt ◽  
Idzi Siatkowski ◽  
Magdalena Magaj ◽  
Agnieszka Tomkowiak ◽  
Jerzy Nawracała
Keyword(s):  
Ionic Liquids ◽  
Plant Regeneration ◽  
Positive Impact ◽  
Microspore Embryogenesis ◽  
Green Plant ◽  
Induction Medium ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
The Impact

Ionic liquids are novel compounds with unique chemical and physical properties. They can be received based on synthetic auxins like 2,4-dichlorophenoxyacetic acid or dicamba, which are commonly used hormones in microspore embryogenesis. Nevertheless, ionic liquids have not been adapted in plant in vitro culture thus far. Therefore, we studied the impact of ionic liquids on the ability to undergo microspore embryogenesis in anther cultures of wheat. Two embryogenic and two recalcitrant genotypes were used for this study. Ten combinations of ionic liquids and 2,4-dichlorophenoxyacetic acid were added to the induction medium. In most cases, they stimulated induction of microspore embryogenesis and green plant regeneration more than a control medium supplemented with only 2,4-dichlorophenoxyacetic acid. Two treatments were the most favorable, resulting in over two times greater efficiency of microspore embryogenesis induction in comparison to the control. The effect of breaking down the genotype recalcitrance (manifested by green plant formation) was observed under the influence of 5 ionic liquids treatments. Summing up, ionic liquids had a positive impact on microspore embryogenesis induction and green plant regeneration, increasing the efficiency of these phenomena in both embryogenic and recalcitrant genotypes. Herbicidal ionic liquids can be successfully used in in vitro cultures.

COLD HARDINESS IN HEXAPLOID TRITICALE

1985 ◽  
Vol 65 (3) ◽  
pp. 487-490 ◽  
Author(s):  
A. E. LIMIN ◽  
J. DVORAK ◽  
D. B. FOWLER
Keyword(s):  
Cold Hardiness ◽  
Tetraploid Wheat ◽  
Triticum Aestivum L ◽  
Hexaploid Triticale ◽  
D Genome ◽  
Potential Source ◽  
Secale Cereale L ◽  
Cold Hardy ◽  
Triticosecale Wittmack ◽  
X Triticosecale Wittmack

The excellent cold hardiness of rye (Secale cereale L.) makes it a potential source of genetic variability for the improvement of this character in related species. However, when rye is combined with common wheat (Triticum aestivum L.) to produce octaploid triticale (X Triticosecale Wittmack, ABDR genomes), the superior rye cold hardiness is not expressed. To determine if the D genome of hexaploid wheat might be responsible for this lack of expression, hexaploid triticales (ABR genomes) were produced and evaluated for cold hardiness. All hexaploid triticales had cold hardiness levels similar to their tetraploid wheat parents. Small gains in cold hardiness of less than 2 °C were found when very non-hardy wheats were used as parents. This similarity in expression of cold hardiness in both octaploid and hexaploid triticales indicates that the D genome of wheat is not solely, if at all, responsible for the suppression of rye cold hardiness genes. There appears to be either a suppressor(s) of the rye cold hardiness genes on the AB genomes of wheat, or the expression of diploid rye genes is reduced to a uniform level by polyploidy in triticale. The suppression, or lack of expression, of rye cold hardiness genes in a wheat background make it imperative that cold-hardy wheats be selected as parents for the production of hardy triticales.Key words: Triticale, Secale, winter wheat, cold hardiness, gene expression

scholarly journals Cytological microevolutive changes in hexaploid triticale (X Triticosecale Wittmack)

Caryologia ◽  
2006 ◽  
Vol 59 (2) ◽  
pp. 112-115
Author(s):  
Ordónez Adriana ◽  
Lorena Elizabeth Torres ◽  
Beatriz Costero ◽  
Ricardo Maich
Keyword(s):  
Hexaploid Triticale ◽  
X Triticosecale ◽  
Triticosecale Wittmack ◽  
X Triticosecale Wittmack

scholarly journals Factors influencing somatic embryogenesis and regeneration ability in somatic tissue culture of spring and winter rye

2008 ◽  
Vol 13 (4) ◽  
pp. 363 ◽  
Author(s):  
R. MA ◽  
S. PULLI
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Developmental Stage ◽  
Embryogenic Callus ◽  
Immature Embryo ◽  
Green Plant ◽  
Somatic Tissue ◽  
Winter Rye ◽  
Effi Ciency

Rye is an important crop in Northern and Eastern Europe. However, the application of various biotechnologies in rye breeding has been limited duo to its recalcitrant in tissue culture. In order to improve somatic tissue effi ciency, key factors affecting somatic embryogenesis and reproducible green plant regeneration of rye (Secale cereale L.) were evaluated and optimised. In this study, a total 27 rye genotypes including 10 spring and 17 winter genotypes were involved in the investigation. Genotype, culture medium, sugar, gel agent and auxin infl uenced somatic embryogenesis of immature embryo signifi cantly. One-two weeks cold pretreatment of young embryo enhanced somatic embryogenesis and green plant regeneration. In culture of immature embryos, infl orescences and leaf segments of the seedlings, explants signifi cantly infl uenced the culture effi ciency. Highest embryogenic callus yield resulted from rye immature embryo as explant compared to young infl orescence and leaf segment of seedling. Developmental stage of embryo played an important role in somatic embryogenesis. Late spherical coleoptile stage (embryo size 0.5–1mm in length) was optimal developmental stage of immature embryo for culture. Morphogenetic potential of embryogenic callus decreased with an increasing number of subcultures, and this ability could be maintained in vitro for a maximum of 8 months of culturing.;

scholarly journals ROLE OF POLYAMINES IN INHIBITION OF ETHYLENE BIOSYNTHESIS AND THEIR EFFECTS ON RICE ANTHER CULTURE DEVELOPMENT

2016 ◽  
Vol 9 (2) ◽  
pp. 60 ◽  
Author(s):  
Iswari S. Dewi ◽  
Bambang S. Purwoko
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Culture Media ◽  
Callus Formation ◽  
Indica Rice ◽  
Ethylene Biosynthesis ◽  
Green Plant ◽  
Culture Development ◽  
Acc Content

The polyamines such as putrescine, spermidine, and spermine were reported to increase green plant regeneration in rice anther culture. Low response of anther culture of rice sub-species indica may be improved with the addition of putrescine in the culture media. Four experiments were conducted to study the role of polyamines in inhibition of ethylene biosynthesis and their effects on rice anther culture development. Anthers of two subspecies of rice, indica (IR64, Krowal, Jatiluhur) and japonica (Taipei 309) were cultured onto media supplemented with putrescine (N6P) and without putrescine (N6). Young<br />panicles containing the anthers at mid-to-late nucleate microspores were cold pretreated at 5 + 2°C and incubated in the dark for 8 days before the anthers were cultured. Results<br />showed that medium without putrescine produced an earlier senescence of indica rice anther than that of japonica. The addition of 10-3 M putrescine into the culture media inhibited ethylene biosynthesis as anther senescence delayed, increased the three polyamines contents, and decreased the ACC content as well as ACC oxydase activity in anther-derived calli. In the anther and anther-derived calli of subspecies indica, the total<br />polyamines content was lower (10.14 nM g-1 anther and 8.48 nM g-1 calli) than that of subspecies japonica (12.61 nM g-1 anther and 10.16 nM g-1 calli), whereas the ethylene production was higher (32.31 nM g-1 anther and 2.48 nM g-1 calli) than the japonica (31.68 nM g-1 anther and 1.76 nM g-1 calli). This study suggests that application of 10-3 M putrescine in anther culture of rice subspecies indica improves androgenesis by inhibiting<br />early senescence of cultured anthers and enhancing embryo or callus formation from microspores.

scholarly journals Efficiency of anther culture technique in the production of wheat double haploids

2008 ◽  
pp. 35-40 ◽  
Author(s):  
Ankica Kondic-Spika ◽  
Borislav Kobiljski ◽  
Nikola Hristov
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Culture Technique ◽  
Average Yield ◽  
Green Plants ◽  
Double Haploids

The objective of the study was to investigate efficiency of anther culture in the production of spontaneous double haploids from randomly selected heterozygous genotypes of wheat (Triticum aestivum L.). Anthers of 20 F1 wheat combinations were grown in vitro on a modified Potato-2 medium. All of the examined genotypes have shown the ability to produce pollen calluses as well as to regenerate green plants. On average for the whole experiment material, 47.2 calluses were produced per 100 cultured anthers. The green plant regeneration ranged from 0.8 to 13.4 green plants per spike, with an overall mean of 5.8. From the total of 582 regenerated green plants, 47.9% (279) were spontaneous double haploids. The final average yield from the study was 2.8 double haploids per spike.

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