EFFECT OF ZEATIN ON in vitro EMBRYOGENESIS AND PLANT REGENERATION FROM ANTHER CULTURE OF HEXAPLOID TRITICALE (× Triticosecale Wittmack)

The experiment was conducted to investigate the performance of three different genotypes (BARI Sarisha-6, BARI Sarisha-8, and BARI Sarisha-11) in two different media viz., MS and B5 with different concentrations of phytohormone (2, 4-D) for callus induction from uninucleate stage anthers of Brassica and subsequent plant regeneration in MS media with different concentrations of phytohormone (BAP and NAA). Among the genotypes, BARI Sarisha-8 showed the best performance for all the parameters of callus induction. The performance of BARI Sarisha-6 was poor compared to others. Maximum rate of callus induction (%) was observed in MS + 0.5 mg/L 2, 4-D followed by B5 + 0.5 mg/L 2,4-D. The media combination MS + 1.0 mg/L BAP 0.3 mg/L 2,4-D showed the best performance for maintenance of calli. Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1. The genotype BARI Sarisha-8 produced higher percent of shoots/calli and required minimum days for shoot initiation. Higher percent calli without shoot were produced by the genotype BARI Sarisha-6. The media combination MS + 2.0 mg/L BAP + 0.5 mg/L NAA showed the best performance for shoot regeneration and required maximum days for shoot initiation. Keywords: Regeneration; BARI Sarisha-6; BARI Sarisha-8; BARI Sarisha-11; anther culture; phytohormone DOI: 10.3329/bjar.v35i2.5896Bangladesh J. Agril. Res. 35(2) : 331-341, June 2010

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EFFECT OF CARBON SOURCE ON IN VITRO PLANT REGENERATION IN ANTHER CULTURE OF CYCLAMEN (CYCLAMEN PERSICUM MILL.)

Acta Horticulturae ◽

10.17660/actahortic.2011.923.18 ◽

2011 ◽

pp. 129-134 ◽

Cited By ~ 1

Author(s):

T. Takamura ◽

K. Sakamoto ◽

M. Horikawa

Keyword(s):

Plant Regeneration ◽

Carbon Source ◽

Anther Culture ◽

In Vitro Plant Regeneration ◽

Cyclamen Persicum ◽

In Vitro Plant

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Haploid plantlet regeneration through anther culture in oilseed Brassica species

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v34i4.5844 ◽

1970 ◽

Vol 34 (4) ◽

pp. 693-703 ◽

Cited By ~ 4

Author(s):

MA Alam ◽

MA Haque ◽

MR Hossain ◽

SC Sarker ◽

R Afroz

Keyword(s):

Plant Regeneration ◽

Anther Culture ◽

Growth Regulators ◽

Callus Induction ◽

Brassica Species ◽

Plantlet Regeneration ◽

Root Induction ◽

Ms Medium ◽

Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009

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In vitro androgenesis in triticale

The EuroBiotech Journal ◽

10.24190/issn2564-615x/2017/01.20 ◽

2017 ◽

Vol 1 (1) ◽

pp. 99-100

Author(s):

Oksana Ivanovna Zaitseva

Keyword(s):

Genetic Variation ◽

Anther Culture ◽

Doubled Haploid ◽

Doubled Haploids ◽

External Factors ◽

Hexaploid Triticale ◽

Doubled Haploid Lines ◽

In Vitro Androgenesis

Abstract The technology for in vitro anther culturing was optimized in hexaploid triticale using combination of external factors that allowed to obtain more than 100 doubled haploid lines. Investigation of genetic variation among anther culture derived doubled haploids of triticale showed the occurrence of heterozygous plants.

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Efficiency of spring barley haploid production in anther culture in vitro: comparison of basic and improved technologies

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v25.1161 ◽

2019 ◽

Vol 25 ◽

pp. 178-183

Author(s):

O. V. Bilinska ◽

P. G. Dulnyev

Keyword(s):

Plant Regeneration ◽

Anther Culture ◽

Spring Barley ◽

Gelling Agent ◽

Improved Method ◽

Cold Pretreatment ◽

Culture In Vitro ◽

Chemically Modified ◽

Modified Starches

Aim. Evaluation of innovative methodological approaches elaborated on model genotypes for usability to increase frequencies of morphogenic structure induction and plant regeneration in anther culture in vitro in spring barley diverse material. Methods. Spikes isolated from F1 and F2 hybrids of four crosses were pretreated using an improved method (4ºC, 28 days), and anthers were inoculated onto nutrient media containing chemically modified starches D5-M and D5a-1 instead of agar. In control cut tillers were emerged in water and pretreated at 4ºC for 5 days. Anthers were cultivated on agar solidified medium. Results. Positive effects of the improved method of cold pretreatment and cultivation of anthers on media solidified with starches were confirmed. The advantage of new gelling agent D5a-1 was proved. Particularly, its usage resulted in a three-fold increase in the frequency of green plant regeneration. Conclusions. In order to increase spring barley androgenic haploid yield, combination of prolonged cold pretreatment with anther cultivation on media solidified with chemically modified starches instead of agar in an integrated technological process is reasonable. Keywords: Hordeum vulgare L., anther culture in vitro, cold pretreatment, starch, agar, embryo formation, plant regeneration.

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Efficiency of anther culture technique in the production of wheat double haploids

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn0815035k ◽

2008 ◽

pp. 35-40 ◽

Cited By ~ 2

Author(s):

Ankica Kondic-Spika ◽

Borislav Kobiljski ◽

Nikola Hristov

Keyword(s):

Triticum Aestivum ◽

Plant Regeneration ◽

Anther Culture ◽

Triticum Aestivum L ◽

Green Plant ◽

Culture Technique ◽

Average Yield ◽

Green Plants ◽

Double Haploids

The objective of the study was to investigate efficiency of anther culture in the production of spontaneous double haploids from randomly selected heterozygous genotypes of wheat (Triticum aestivum L.). Anthers of 20 F1 wheat combinations were grown in vitro on a modified Potato-2 medium. All of the examined genotypes have shown the ability to produce pollen calluses as well as to regenerate green plants. On average for the whole experiment material, 47.2 calluses were produced per 100 cultured anthers. The green plant regeneration ranged from 0.8 to 13.4 green plants per spike, with an overall mean of 5.8. From the total of 582 regenerated green plants, 47.9% (279) were spontaneous double haploids. The final average yield from the study was 2.8 double haploids per spike.

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Spike morphology alternations in androgenic progeny of hexaploid triticale (× Triticosecale Wittmack) caused by nullisomy of 2R and 5R chromosomes

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-019-10021-7 ◽

2019 ◽

Vol 56 (2) ◽

pp. 150-158

Author(s):

Michał T. Kwiatek ◽

Zofia Banaszak ◽

Roksana Skowrońska ◽

Danuta Kurasiak-Popowska ◽

Sylwia Mikołajczyk ◽

...

Keyword(s):

Quantitative Traits ◽

Chromosome Doubling ◽

Spike Length ◽

Hexaploid Triticale ◽

Spike Morphology ◽

Complete Set ◽

Triticosecale Wittmack ◽

Stimulation Of

AbstractInduction of androgenesis, followed by chromosome doubling, is a crucial method to obtain complete homozygosity in one-generation route. However, in vitro androgenesis can result in various genetic and epigenetic changes in derived triticale plants. In this study, we evaluated chromosome alternations and we associated them with the changes of spike morphology in androgenic progeny of triticale. We karyotyped offspring plants that derived from double haploid plants using fluorescence in situ hybridization techniques. We distinguished four major groups of karyotypes: double ditelosomics, nullisomics N2R, nullisomics N5R, and triticale plants with a complete set of chromosomes. It is known that more than half of QTLs connected with androgenic response are located in R-genome of triticale but 2R, 5R, and 6R chromosomes are not included. We hypothesized that the reason why only aberrations of chromosomes 2R and 5R appear during androgenesis of triticale is that because these chromosomes are not involved in the stimulation of androgenic response and the following regeneration of plants is not disrupted. Concerning the established groups, we evaluated following quantitative traits: spike length, number of spikes per plant, number of spikelets per spike, and number of grains per spike. The nullisomy of chromosome 2R and 5R resulted in vast changes in spike architecture of triticale plants, which can be correlated with the location of major QTLs for spike morphology traits on these chromosomes. The spikes of nullisomic plants had significantly decreased spike length which correlated with the reduction of number of spikelets per spike and number of grains per spike.

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