Abscisic acid promotes shoot regeneration in Arabidopsis zygotic embryo explants

The hairy vetch (Vicia villosa Roth) is a climbing, prostrate or trailing legume grown as forage.It fixes atmospheric nitrogen, reduces soil erosion and provides an instant mulch. Multiple axillary shoot regeneration from a mature seed explant (zygotic embryo with two cotyledons) was obtained on MS medium containing 0.05 - 1.6 mg/l TDZ with or without 0.10 mg/l IBA. The frequency (%) of shoot regeneration ranged from 45.83-75.00% with a maximum number of 28.6 shoots per explant on MS medium containing 0.20 mg/l TDZ-0.10 mg/l IBA. The mean shoot length decreased proportionately with each increase in TDZ concentration irrespective of the IBA concentration in the culture medium. However, comparing the two types of regeneration media, longer shoots were recorded in the presence of IBA in the culture medium. Regenerated shoots were pulse treated with 50 mg/l IBA for 5, 10 and 20 min for rooting.

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Improved Shoot Regeneration from Root Explants Using an Abscisic Acid-Containing Medium

Plant Cell Culture Protocols - Methods in Molecular Biology ◽

10.1007/978-1-61779-818-4_15 ◽

2012 ◽

pp. 183-189 ◽

Cited By ~ 4

Author(s):

Subramanian Paulraj ◽

Edward C. Yeung

Keyword(s):

Abscisic Acid ◽

Shoot Regeneration ◽

Root Explants

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Light Inhibition of Shoot Regeneration Is Regulated by Endogenous Abscisic Acid Level in Calli Derived from Immature Barley Embryos

PLoS ONE ◽

10.1371/journal.pone.0145242 ◽

2015 ◽

Vol 10 (12) ◽

pp. e0145242 ◽

Cited By ~ 4

Author(s):

Kazuhide Rikiishi ◽

Takakazu Matsuura ◽

Yoko Ikeda ◽

Masahiko Maekawa

Keyword(s):

Abscisic Acid ◽

Shoot Regeneration ◽

Acid Level ◽

Endogenous Abscisic Acid ◽

Light Inhibition ◽

Abscisic Acid Level

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PCIB Enhances Regeneration of Ipomoea cordatotriloba Dennstedt Leaf Explants and Protoplasts

HortScience ◽

10.21273/hortsci.29.4.327 ◽

1994 ◽

Vol 29 (4) ◽

pp. 327-328

Author(s):

Ruth S. Kobayashi ◽

John C. Bouwkamp ◽

Stephen L. Sinden

Keyword(s):

Abscisic Acid ◽

Shoot Regeneration ◽

Callus Induction ◽

Leaf Explants ◽

Dichlorophenoxyacetic Acid ◽

Regeneration Medium ◽

Ipomoea Cordatotriloba ◽

2,4 Dichlorophenoxyacetic Acid ◽

Chlorophenoxyisobutyric Acid

Leaf callus of Ipomoea cordatotriloba was initiated by culturing explants on Linsmaier and Skoog medium containing 3 g yeast extract/liter, 18.9 μm ABA, 2.3 μm 2,4-D, and 0.15 m sucrose. Calluses were transferred to Murashige and Skoog media containing 17.8 μm BA and 0, 1, 10, or 100 μm PCIB. The number of shoots from calluses grown on medium containing 10 μm PCIB increased significantly, and the percentage of calluses exhibiting shoot regeneration almost doubled compared to calluses grown on regeneration medium without PCIB. Protoplasts isolated from stem and petiole tissues of in vitro-grown plants were cultured in Kao and Michayluk 8p medium to the callus stage. Calluses (4-6 mm) were transferred to the callus induction and regeneration media used to regenerate leaf-explant callus. Of the protoplast-derived calluses cultured on media containing 10 or 100 μm PCIB, ≈13% and 18%, respectively, regenerated shoots after 2 months; none regenerated on the medium without PCIB. Chemical names used: abscisic acid (ABA); 2,4-dichlorophenoxyacetic acid (2,4-D); N6-benzyladenine (BA); α -p-chlorophenoxyisobutyric acid (PCIB).

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