Effectiveness of Cover Crop Termination Methods on No-Till Cantaloupe

In a no-till system, there are many different methods available for terminating cover crops. Mechanical termination, utilizing rolling and crimping technology, is one method that injures the plant without cutting the stems. Another popular and commercially available method is mowing, but this can cause problems with cover crop re-growth and loose residue interfering with the planter during cash crop planting. A field experiment was conducted over three growing seasons in northern Alabama to determine the effects of different cover crops and termination methods on cantaloupe yield in a no-till system. Crimson clover, cereal rye, and hairy vetch cover crops were terminated using two different roller-crimpers, including a two-stage roller-crimper for four-wheel tractors and a powered roller-crimper for a two-wheel walk-behind tractor. Cover crop termination rates were evaluated one, two, and three weeks after termination. Three weeks after rolling, a higher termination rate was found for flail mowing (92%) compared to lower termination rates for a two-stage roller (86%) and powered roller-crimper (85%), while the control termination rate was only 49%. There were no significant differences in cantaloupe yield among the rolling treatments, which averaged 38,666 kg ha−1. However, yields were higher for cereal rye and hairy vetch cover crops (41,785 kg ha−1 and 42,000 kg ha−1) compared to crimson clover (32,213 kg ha−1).

A Comparison of Drill and Broadcast Planting Methods for Biomass Production of Two Legume Cover Crops

The effects of drill and broadcast planting methods on cover crop biomass production depend on various environmental and operational factors. We investigated whether drilling and broadcasting result in different amounts of biomass production by crimson clover (Trifolium incarnatum L.) and hairy vetch (Vicia villosa Roth) in the upstate of South Carolina, and results vary when seeding rates are increased by 50% from the standard value (22.4 kg ha−1). Field trials were conducted during the fall–winter of 2019–2020 (season one) and 2020–2021 (season two) at the Piedmont Research and Education Center in Pendleton, SC, USA. Cover crop (hairy vetch, crimson clover), planting method (broadcast, drill), and seeding rate (standard, high) treatments were arranged as a 2 × 2 × 2 factorial in both years. Aboveground biomass was measured after 22.5 weeks from planting. At standard seeding rates, crimson clover produced a higher biomass when drilled, rather than broadcasted, whereas biomass production did not vary for hairy vetch. Even with 50% higher seeding rates, broadcasting did not always produce the same biomass as that of drilling for crimson clover. Our results suggest that the advantage of drilling over broadcasting depends upon the cover crop species, as crimson clover responds well to drilling, whereas hairy vetch does not.

MACROAGGREGATION OF A PALEUDALF AFFECTED BY CAVITATION INTENSITY AND MANAGEMENT SYSTEMS WITH COVER PLANTS

Ultrasonic-based techniques allow the prediction of the energy required to break the aggregate and have been more commonly used to measure the stability of aggregates. Although they result in the same applied energy, certain combinations of time and power might correspond to different intensities of cavitation. Consequently, different responses in aggregation indexes can be obtained with different configurations of ultrasound techniques. Thus, this work was carried out to evaluate the effects of cavitation intensity in the distribution of aggregates of a Paleudalf under management systems with cover plants and to compare aggregate stability determination methods (ultrasound versus wet sieving). Aggregate samples of the BS (bare soil), OT (black oat + forage turnips) and OV (black oat + hairy vetch) treatments were exposed to ultrasonic irradiation in different combinations of potency and time: (U1) 74.5 W/4 s; (U2) 49.7 W/6 s; (U3) 74.5 W/10 s and (U4) 49.7 W/15 s. After each sonification, the samples were passed in the same set of sieves used in the standard method of wet sieving -WS (8.00 - 4.76, 4.76 - 2.00, 2.00 - 1.00, 1.00 - 0.25 and < 0.25 mm) and the geometric mean diameter (GMD) and mass-weighted mean diameter (WMD) were calculated. The amplitude of vibration exerted a greater influence on soil breakdown than the total energy applied. Compared to the ultrasound method, in the WS method observed higher percentage of retained aggregates in the size class 8-4.76 mm and, consequently, greater aggregation indexes GMD and WMD.

L-Canavanine, a Root Exudate From Hairy Vetch (Vicia villosa) Drastically Affecting the Soil Microbial Community and Metabolite Pathways

L-Canavanine, a conditionally essential non-proteinogenic amino acid analog to L-arginine, plays important roles in cell division, wound healing, immune function, the release of hormones, and a precursor for the synthesis of nitric oxide (NO). In this report, we found that the L-canavanine is released into the soil from the roots of hairy vetch (Vicia villosa) and declines several weeks after growth, while it was absent in bulk proxy. Hairy vetch root was able to exudate L-canavanine in both pots and in vitro conditions in an agar-based medium. The content of the L-canavanine in pots and agar conditions was higher than the field condition. It was also observed that the addition of L-canavanine significantly altered the microbial community composition and diversity in soil. Firmicutes and Actinobacteria became more abundant in the soil after the application of L-canavanine. In contrast, Proteobacteria and Acidobacteria populations were decreased by higher L-canavanine concentration (500 nmol/g soil). Prediction of the soil metabolic pathways using PICRUSt2 estimated that the L-arginine degradation pathway was enriched 1.3-fold when L-canavanine was added to the soil. Results indicated that carbon metabolism-related pathways were altered and the degradation of nitrogen-rich compounds (i.e., amino acids) enriched. The findings of this research showed that secretion of the allelochemical L-canavanine from the root of hairy vetch may alter the soil microbial community and soil metabolite pathways to increase the survival chance of hairy vetch seedlings. This is the first report that L-canavanine acts as an allelochemical that affects the biodiversity of soil microbial community.

Response of broadleaf and grass cover crop species to soil residues of glyphosate and aminomethylphosphonic acid (AMPA)

Glyphosate is the most widely used herbicide in the United States; however, concern about increasing residues of glyphosate and its metabolite aminomethylphosphonic acid (AMPA) in soil is escalating. There is a lack of scientific literature examining the response of cover crops to soil residues of glyphosate or AMPA. The objectives of this study were to evaluate the impact of glyphosate or AMPA residues in silty clay loam soil on emergence, growth, and biomass of cover crops, including cereal rye, crimson clover, field pea, hairy vetch, and winter wheat, as well as their germination in a 0.07% (0.7 g/L) solution of AMPA or glyphosate. Greenhouse studies were conducted at the University of Nebraska-Lincoln to determine the dose response of broadleaf and grass cover crops to soil-applied glyphosate or AMPA. The results indicated that soil treated with glyphosate or AMPA up to 105 mg ae kg–1 of soil had no effect on the emergence, growth, above-ground biomass, and root biomass of any of the cover crop species tested. To evaluate the impact of AMPA or glyphosate on the seed germination of cover crop species, seeds were soaked in petri plates filled with a 0.7 g L−1 solution of AMPA or glyphosate. There was no effect of AMPA on seed germination of any of the cover crop species tested. Seed germination of crimson clover and field pea in a 0.7 g L−1 solution of glyphosate was comparable to the nontreated control; however, the germination of cereal rye, hairy vetch, and winter wheat was reduced by 48%, 75%, and 66%, respectively, compared to the nontreated control. The results suggested that glyphosate or AMPA up to 105 mg ae kg–1 in silt clay loam soil is unlikely to cause any negative effect on the evaluated cover crop species.

Evaluating The Performance of Nutrients Uptake Capabilities and Enzymatic Activities in The Different Green Manures

Winter green manure potentially uptake large amount of phosphorus (P) and potassium (K) from the soil. However, the mechanism underlying nutrient uptake in green manure varieties remain elusive. This study illustrates the P and K uptake potential in different leguminous and non-leguminous species through integrated approaches. The designed studies evaluated the 10 green manure crops included five leguminous species (i.e smooth vetch hairy vetch, common vetch, lathyrus sativus, and milk vetch) and five non-leguminous species (i.e February orchid, chinese radish, forage radish, rapeseed and ryegrass) were planted. Experimental results indicated that the smooth vetch had highest P and K uptake capabilities among all leguminous species. While, rapeseed and chinese radish greatly P and K obtain among all non-leguminous treatments. Therefore various species also affected soil P and K availability and soil enzymes. Soil enzymatic activities including phosphatase (150.7%) and N-acetyl-glucosaminidase (95.4%) were elevated in lathryus sativus compared to the hairy vetch and common vetch respectively. The highest β-glucosidase (95.2%) and leucine-aminopeptidase (107.6%) activities accumulated in lathyrus sativus greater than the smooth vetch in all legumes species. In non-leguminous treatments phosphatase (500.5%), β-glucosidase (424.4%), and N-acetylglucosaminidase (256.3%) activities were improved in chinese radish as compared to the rapeseed. In constrast ryegrass was promoted greatest leucine-aminopeptidase activity by 182.7% higher than forage radish. However soil enzymes significanlty improve soil nutrient status. As a conclusion smooth vetch, rapeseed, and Chinese radish varieties have more P and K obtain capacity and forage radish could enhance soil enzyme activities. It is suggested these varieties might be a better choice in green manuring practice for improving nutrient management and soil nutrient quality in agro-ecosystems.

Effects of green-manure and tillage management on soil microbial community composition, nutrients and tree growth in a walnut orchard

This study characterized the effect of green manures (February orchid, hairy vetch, rattail fescue and a no-green-manure control) and the termination method (flail or disk) on nutrient contents, enzyme activities, microbial biomass, microbial community structure of rhizosphere soil and vegetative growth of walnut tree. All three selected green manures significantly enhanced the water content, organic C, total N and available P. The rattail fescue significantly decreased the mineral N. Total organic C, total N, mineral N and available P were significantly greater under flail than under disk. Hairy vetch and February orchid significantly improved levels of soil β-glucosidase, N-acetyl-glucosaminidase and acid phosphatase activity, whereas rattail fescue improved only β-glucosidase activity. All of the green manures significantly decreased phenoloxidase activity. β-glucosidase, N-acetyl-glucosaminidase and acid phosphatase activities were significantly greater under flail relative to disk. The termination method had no significant effect on phenoloxidase activity. The different types of green manures and termination methods significantly altered the soil microbial biomass and microbial community structure. The green-manure treatments were characterized by a significantly greater abundance of Gram-positive (Gram +) bacteria, total bacteria and saprophytic fungi compared to the control. Hairy vetch significantly decreased the abundance of arbuscular mycorrhizal fungi (AMF) while February orchid and rattail fescue increased their abundance compared to the no-green-manure treatment. The abundance rates of Gram+ bacteria, actinomycetes, saprophytic fungi and AMF were significantly greater in soils under flail than under disk. In terms of vegetative growth of walnut tree, hairy vetch showed the greatest positive effects. The growth of walnut tree was significantly greater under flail relative to disk. Our results indicate that green-manure application benefits the rhizosphere soil micro-ecology, rhizosphere soil nutrient contents and tree growth. Overall, the hairy vetch and flail combined treatment is recommended for walnut orchards in northern China.

Impact of Cover Crop Monocultures and Mixtures on Organic Carbon Contents of Soil Aggregates

Cover crops are considered an integral component of agroecosystems because of their positive impacts on biotic and abiotic indicators of soil health. At present, we know little about the impact of cover crop types and diversity on the organic carbon (OC) contents of different soil aggregate-size classes. In this study, we investigated the effect of cover plant diversity on OC contents of different soil aggregates, such as macro- (<2000–500 μm), meso- (<500–250 μm), and micro-aggregates (<250 μm). Our experiment included a total of 12 experimental treatments in triplicate; six different monoculture treatments such as chickling vetch (Vicia villosa), crimson clover (Trifolium incarnatum), hairy vetch (Vicia villosa), field peas (Pisum sativum), oilseed radish (Raphanus sativus), and mighty mustard (Brassica juncea), and their three- and six-species mixture treatments, including one unplanted control treatment. We performed this experiment usingdeep pots that contained soil collected from a corn-soybean rotation field. At vegetative maturity of cover plants (about 70 days), we took soil samples, and the soil aggregate-size classes were separated by the dry sieving. We hypothesized that cover crop type and diversity will improve OC contents of different soil aggregate-size classes. We found that cover plant species richness weakly positively increased OC contents of soil macro-aggregates (p = 0.056), whereas other aggregate-size classes did not respond to cover crop diversity gradient. Similarly, the OC contents of macroaggregates varied significantly (p = 0.013) under cover crop treatments, though neither monoculture nor mixture treatments showed significantly higher OC contents than the control treatment in this short-term experiment. Interestingly, the inclusion of hairy vetch and oilseed radish increased and decreased the OC contents of macro- and micro-aggregates, respectively. Moreover, we found a positive correlation between shoot biomass and OC contents of macroaggregates. Overall, our results suggest that species-rich rather than -poor communities may improve OC contents of soil macroaggregates, which constitute a major portion of soil systems, and are also considered as important indicators of soil functions.