Improved Shoot Regeneration from Root Explants Using an Abscisic Acid-Containing Medium

The medicinal plant, Aspilia africana, has been traditionally used in several African countries to treat many diseases such as tuberculosis, cough, inflammation, malaria, osteoporosis, and diabetes. In this study, we developed a protocol for in vitro propagation of A. africana using indirect shoot organogenesis from leaf and root explants of in vitro-grown seedlings and assessed the tissues at different developmental stages. The highest callus induction (91.9 ± 2.96%) from leaf explants was in the Murashige and Skoog (MS) medium augmented with 1.0 mg/L 6-Benzylaminopurine (BAP) and 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) while from root explants, the highest callus induction (92.6 ± 2.80%) was in the same plant tissue culture medium augmented with 0.5 mg/L BAP and 1.0 mg/L 2,4-D. The best shoot regeneration capacity from leaf-derived calli (i.e., 80.0 ± 6.23% regeneration percentage and 12.0 ± 6.23 shoots per callus) was obtained in medium augmented with 1.0 mg/L BAP and 0.05 mg/L α-Naphthaleneacetic acid (NAA); the best regeneration capacity for root-derived calli (i.e., 86.7 ± 6.24% shoot regeneration percentage and 14.7 ± 1.11 shoots per callus) was obtained in the MS medium augmented with 1.0 mg/L BAP, 0.05 mg/L NAA, and 0.1 mg/L Thidiazuron (TDZ). Regenerated plantlets developed a robust root system in 1/2 MS medium augmented with 0.1 mg/L NAA and had a survival rate of 93.6% at acclimatization. The in vitro regenerated stem tissue was fully differentiated, while the young leaf tissue consisted of largely unorganized and poorly differentiated cells with large intercellular airspaces typical of in vitro leaf tissues. Our study established a protocol for the indirect regeneration of A. africana and offers a basis for its domestication, large-scale multiplication, and germplasm preservation. To the best of our knowledge, this is the first study to develop an indirect regeneration protocol for A. africana and conduct anatomical assessment through the different stages of development from callus to a fully developed plantlet.

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Light Inhibition of Shoot Regeneration Is Regulated by Endogenous Abscisic Acid Level in Calli Derived from Immature Barley Embryos

PLoS ONE ◽

10.1371/journal.pone.0145242 ◽

2015 ◽

Vol 10 (12) ◽

pp. e0145242 ◽

Cited By ~ 4

Author(s):

Kazuhide Rikiishi ◽

Takakazu Matsuura ◽

Yoko Ikeda ◽

Masahiko Maekawa

Keyword(s):

Abscisic Acid ◽

Shoot Regeneration ◽

Acid Level ◽

Endogenous Abscisic Acid ◽

Light Inhibition ◽

Abscisic Acid Level

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Adventitious shoot regeneration from leaf, stem and root explants of commercial cultivars of Gentiana

Plant Cell Reports ◽

10.1007/bf00232456 ◽

1996 ◽

Vol 15 (8) ◽

pp. 578-581 ◽

Cited By ~ 37

Author(s):

Keizo Hosokawa ◽

Masaru Nakano ◽

Yayoi Oikawa ◽

Saburo Yamamura

Keyword(s):

Shoot Regeneration ◽

Adventitious Shoot ◽

Adventitious Shoot Regeneration ◽

Root Explants ◽

Commercial Cultivars

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High frequency plant regeneration of Chrysopogon zizanioides via organogenesis and somatic embryogenesis-an underutilized pharmaceutically valuable biofuel plant

10.21203/rs.3.rs-934878/v1 ◽

2021 ◽

Author(s):

M. Merlin Monisha ◽

M. Prakash ◽

K.R. Saravanan ◽

Anandan R

Keyword(s):

Somatic Embryogenesis ◽

Somatic Embryo ◽

Shoot Regeneration ◽

Adventitious Shoots ◽

Leaf Explants ◽

Ms Medium ◽

Natural Habitats ◽

Root Explants ◽

Chrysopogon Zizanioides ◽

Wide Range

Abstract Vetiver (Chrysopogon zizanioides) is an essential oil-producing plant that has tremendous application in cosmetics, perfumery, and herbal medicine. Natural sterility and indiscriminate harvests lead to the risk of extinction of plant species in natural habitats. Therefore, a protocol for regeneration systems via organogenesis and somatic embryogenesis using node, leaf, and root explants has been standardized. The highest shoot regeneration frequency (72.2%) through organogenesis was attained from node explants on MS (Murashige & Skoog) medium comprising 2.0 mg L-1 BAP (“6-benzylaminopurine”). Concurrently, leaf explants cultivated on MS medium augmented by 1.0 mg L-1, 2, 4-D (“2, 4-dichlorophenoxyacetic acid”) formed the optimal frequency (75.35%) of white friable compact (WFC) callus. However, the root explant was less responsive for WFC callus induction. Organogenic WFC callus cultivated on MS medium fortified by kinetin (1.0 mg L-1) as well as BAP (1.0 mg L-1) revealed the highest shoot regeneration efficiency (75.49%) with 48 shoots per callus. Adventitious shoots obtained from node and WFC callus of both leaf and root explants cultivated on MS medium increased by NAA (2.0 mg L-1 showed the optimal rooting of 76.97%. Concomitantly, an elevated frequency of somatic embryogenesis (52.50%) was recorded from leaf explants on MS medium using BAP (0.5 mg L-1) & 2, 4-D (1.0 mg L-1). Leaf explants were superior to node and root explants for somatic embryo initiation. The cotyledonary embryos were efficiently germinated into complete plantlets on a hormone-free MS medium. The plantlets gathered from organogenesis & somatic embryo genesis was effectively acclimatized into phenomenally similar plants. This technique may be applicable for wide-range propagation, genetic engineering, and the formation of bioactive compounds.

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Adventitious shoot regeneration from leaf, petiole and root explants in triploid (Populus alba × P. glandulosa)× P. tomentosa

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-019-01608-4 ◽

2019 ◽

Vol 138 (1) ◽

pp. 121-130 ◽

Cited By ~ 2

Author(s):

Qingqing Zeng ◽

Zhiqiang Han ◽

Xiangyang Kang

Keyword(s):

Shoot Regeneration ◽

Adventitious Shoot ◽

Populus Alba ◽

Adventitious Shoot Regeneration ◽

Root Explants ◽

Leaf Petiole

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Effects of plant growth regulators on lentil (Lens culinaris Medik.) cultivars

Bangladesh Journal of Botany ◽

10.3329/bjb.v44i1.22727 ◽

2015 ◽

Vol 44 (1) ◽

pp. 79-84 ◽

Cited By ~ 1

Author(s):

Fethi Ahmet Ozdemir ◽

Musa Turker ◽

Khalid Mahmood Khawar

Keyword(s):

Plant Growth ◽

Genetic Transformation ◽

Shoot Regeneration ◽

Lens Culinaris ◽

Culture Media ◽

Economic Importance ◽

Ms Medium ◽

Root Explants ◽

Legume Crop ◽

Internode Explants

Lentil (Lens culinaris Medik.) is an annual pulse legume crop of immense economic importance. This study reports use of 16 lentil cultivars' shoot tips, internode, hypocotyl, cotyledon and root explants cultured on MS medium containing 3 mg/l BAP-0.5 mg/l 2,4-D or 0.25 mg/l IBA for shoot regeneration. The results showed maximum shoot regeneration from cv. Yesil 21 on internode explants on 3mg/l BAP-0.5 mg/l 2,4-D. Best shoot regeneration on MS medium containing 0.25 mg/l IBA was noted on internode explants of cv. Seyran 96. In general shoot regeneration on 0.25 mg/l IBA was better compared to 3mg/l BAP and 0.5 mg/l 2,4-D. Well developed shoots from all cultivars regenerated on both types of culture media were rooted on MS medium containing 0.19 mg/l NAA. A system to regenerate lentil is established that will help in easy genetic transformation in future. DOI: http://dx.doi.org/10.3329/bjb.v44i1.22727 Bangladesh J. Bot. 44(1): 79-84, 2015 (March

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PCIB Enhances Regeneration of Ipomoea cordatotriloba Dennstedt Leaf Explants and Protoplasts

HortScience ◽

10.21273/hortsci.29.4.327 ◽

1994 ◽

Vol 29 (4) ◽

pp. 327-328

Author(s):

Ruth S. Kobayashi ◽

John C. Bouwkamp ◽

Stephen L. Sinden

Keyword(s):

Abscisic Acid ◽

Shoot Regeneration ◽

Callus Induction ◽

Leaf Explants ◽

Dichlorophenoxyacetic Acid ◽

Regeneration Medium ◽

Ipomoea Cordatotriloba ◽

2,4 Dichlorophenoxyacetic Acid ◽

Chlorophenoxyisobutyric Acid

Leaf callus of Ipomoea cordatotriloba was initiated by culturing explants on Linsmaier and Skoog medium containing 3 g yeast extract/liter, 18.9 μm ABA, 2.3 μm 2,4-D, and 0.15 m sucrose. Calluses were transferred to Murashige and Skoog media containing 17.8 μm BA and 0, 1, 10, or 100 μm PCIB. The number of shoots from calluses grown on medium containing 10 μm PCIB increased significantly, and the percentage of calluses exhibiting shoot regeneration almost doubled compared to calluses grown on regeneration medium without PCIB. Protoplasts isolated from stem and petiole tissues of in vitro-grown plants were cultured in Kao and Michayluk 8p medium to the callus stage. Calluses (4-6 mm) were transferred to the callus induction and regeneration media used to regenerate leaf-explant callus. Of the protoplast-derived calluses cultured on media containing 10 or 100 μm PCIB, ≈13% and 18%, respectively, regenerated shoots after 2 months; none regenerated on the medium without PCIB. Chemical names used: abscisic acid (ABA); 2,4-dichlorophenoxyacetic acid (2,4-D); N6-benzyladenine (BA); α -p-chlorophenoxyisobutyric acid (PCIB).

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