Developing a highly efficient regeneration system for leaves of tissue-cultured tetraploid Robinia pseudoacacia L.

Author(s):  
Xiuyu Li ◽  
Qi Guo ◽  
Chao Han ◽  
Juan Li ◽  
Li Dong ◽  
...  
2011 ◽  
Vol 10 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Gui-xiang YIN ◽  
Yan-li WANG ◽  
Mao-yun SHE ◽  
Li-pu DU ◽  
Hui-jun XU ◽  
...  

2013 ◽  
Vol 93 (6) ◽  
pp. 1029-1035 ◽  
Author(s):  
Wei Hua ◽  
Jinghuan Zhu ◽  
Yi Shang ◽  
Junmei Wang ◽  
Qiaojun Jia ◽  
...  

Hua, W., Zhu, J., Shang, Y., Wang, J., Jia, Q., Lin, F. and Yang, J. 2013. Establishment of a highly efficient regeneration method from the scraped broken embryo of mature barley seed. Can. J. Plant Sci. 93: 1029–1035. An efficient and reproducible plant regeneration system for mature barley would represent a significant innovation for barley tissue culture and genetic transformations to optimize barley crop traits. To address this need, we used scraped broken embryos (SBEs) from dried barley seeds to establish a highly efficient regeneration system. Mature SBEs cultured in an induction medium with 2 mg L−1 2,4-D offered the best callus formation (92.99% induction efficiency) and no germination. Furthermore, calli arising from SBEs had the highest regeneration frequency (69.72%) in FHG medium with 1 mg L−1 6-benzylaminopurine. We then compared three different cultivars: Golden Promise, Zaoshu3, and Zhepi8 for callogenesis and regeneration using an established protocol. Zhepi8 had the greatest callus induction (92.78%) and regeneration (68.24%). Our innovative methods for mature barley plant reproduction will contribute to improvements in genetic barley transformation as an alternate regeneration system.


Pneumologie ◽  
2004 ◽  
Vol 58 (11) ◽  
Author(s):  
S Kespohl ◽  
R Merget ◽  
M Gellert ◽  
T Brüning ◽  
M Raulf-Heimsoth

Author(s):  
V.V. Tanyukevich ◽  
◽  
S.V. Tyurin ◽  
D.V. Khmeleva ◽  
A.A. Kvasha ◽  
...  

Works on protective afforestation are carried out in order to protect agricultural land from degradation processes, as well as to improve the microclimate of land. The research purpose is to study the bioproductivity and environmental role of Robinia pseudoacacia L. forest shelterbelts in the conditions of the Kuban lowland. The approved and generally accepted methods of forest valuation, forest land reclamation, botany, and mathematical statistics were applied. Plantings were created according to the standard technology for the steppe zone of the Russian Federation. The area of forest shelterbelts is 62.4 ths ha, including 5 % of the young growth (I state class), 80 % of middle-aged forest plantings (II state class), 10 % of maturing plantings (II state class), 5 % of mature and overmature plantings (III state class). Living ground cover is formed by the following species: Koeleria pyramidata L., Poa pratensis L., Festuca pratensis H., Elytrígia repens L., Dactylis glomerata L., and Phlum pratense L. Aboveground phytomass is 100–300 g/m2; height is 25–32 cm. Plantings are characterized by the quality classes: young growth – I and II; middle-aged and maturing – III; mature and overmature – IV. At the age of natural maturity (70 years), the Robinia trunk reaches the average height of 15.1 m with the average diameter of 22.1 cm. The total stock of wood reaches 18, (ths m3), including (ths m3): young growth – 68 (ths m3); middleaged plantings – 14,871 (ths m3); maturing plantings – 2,187 (ths m3); mature and overmature plantings – 1,314 (ths m3). Aboveground phytomass in young growth is 20.2 t/ha; in mature and overmature plantings it is 391.2 t/ha. In the region it is estimated at 17,070 ths t, including (ths t): young growth – 64; middle-aged plantings – 13,753; maturing plantings – 2,032; mature and overmature plantings – 1,221. The share of stem mass reaches 84.5–80.8 %; woody greenery – 4.2–1.5 %; branches – 11.3–17.7 %. Recalculation coefficients of the stock into aboveground phytomass are the following for: young growth – 0.936; mature and overmature forest shelterbelts – 0.929. Phytosaturation of forest shelterbelts varies within 0.314–2.474 kg/m3. Forest shelterbelts have accumulated 8,534 ths t of carbon, which is estimated at 145.1 mln dollars. The sphere of application of the research results is the Krasnodar Krai forestry, which is recommended to create an additional 60 ths ha of forest shelterbelts, which will provide a normative protective forest cover of arable land of 5 % and annual carbon sequestration up to 3.4 t/ha.


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