Berberine inhibits norepinephrine-induced apoptosis in neonatal rat cardiomyocytes via inhibiting ROS-TNF-α-caspase signaling pathway

2012 ◽  
Vol 19 (6) ◽  
pp. 424-431 ◽  
Author(s):  
Xiu-xiu Lv ◽  
Xiao-hui Yu ◽  
Hua-dong Wang ◽  
Yu-xia Yan ◽  
Yan-ping Wang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Tnf Α ◽  
Induced Apoptosis

Nitric oxide signaling in stretch‐induced apoptosis of neonatal rat cardiomyocytes

2006 ◽  
Vol 20 (11) ◽  
pp. 1883-1885 ◽  
Author(s):  
Xudong Liao ◽  
Jun‐Ming Liu ◽  
Lei Du ◽  
Aihui Tang ◽  
Yingli Shang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Nitric Oxide Signaling ◽  
Neonatal Rat Cardiomyocytes ◽  
Induced Apoptosis

Minoxidil Attenuates Ischemia-Induced Apoptosis in Cultured Neonatal Rat Cardiomyocytes

2004 ◽  
Vol 43 (6) ◽  
pp. 789-794 ◽  
Author(s):  
Tomoka Takatani ◽  
Kyoko Takahashi ◽  
Chengshi Jin ◽  
Takahisa Matsuda ◽  
Xinyao Cheng ◽  
...  
Keyword(s):  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Induced Apoptosis

L-carnitine reduces doxorubicin-induced apoptosis through a prostacyclin-mediated pathway in neonatal rat cardiomyocytes

2011 ◽  
Vol 146 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Hung-Hsin Chao ◽  
Ju-Chi Liu ◽  
Hong-Jye Hong ◽  
Jia-wei Lin ◽  
Cheng-Hsien Chen ◽  
...  
Keyword(s):  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Induced Apoptosis

Tanshinone IIA protects neonatal rat cardiomyocytes from adriamycin-induced apoptosis

2008 ◽  
Vol 151 (2) ◽  
pp. 79-87 ◽  
Author(s):  
Jie Gao ◽  
Guoqing Yang ◽  
Rongbiao Pi ◽  
Ruifang Li ◽  
Ping Wang ◽  
...  
Keyword(s):  
Tanshinone Iia ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Induced Apoptosis

Morphine mimics the antiapoptotic effect of preconditioning via an Ins(1,4,5)P3 signaling pathway in rat ventricular myocytes

2005 ◽  
Vol 288 (1) ◽  
pp. H83-H88 ◽  
Author(s):  
Stéphanie Barrère-Lemaire ◽  
Nicolas Combes ◽  
Catherine Sportouch-Dukhan ◽  
Sylvain Richard ◽  
Joël Nargeot ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Signaling Pathway ◽  
Dna Fragmentation ◽  
Ventricular Myocytes ◽  
Neonatal Rat ◽  
Cytochrome C Release ◽  
Rat Cardiomyocytes ◽  
Rat Ventricular Myocytes ◽  
Antiapoptotic Effect ◽  
Induced Apoptosis

Morphine has cardioprotective effects against ischemic-reperfusion injuries. This study investigates whether morphine could mimic the antiapoptotic effect of preconditioning using a model of cultured neonatal rat cardiomyocytes subjected to metabolic inhibition (MI). To quantify MI-induced apoptosis, DNA fragmentation and mitochondrial cytochrome c release levels were measured by ELISA. MI-dependent DNA fragmentation was prevented by both Z-VAD-fmk (20 μM), a pan-caspase inhibitor, and cyclosporine A (CsA; 5 μM), a mitochondrial pore transition blocker, added during MI (36% and 54% decrease, respectively). MI-dependent cytochrome c release was not blocked by Z-VAD-fmk but was decreased (38%) by CsA during MI. Metabolic preconditioning (MIP) and preconditioning with morphine (1 μM) were also assessed. MI-dependent DNA fragmentation and cytochrome c release were prevented by MIP (40% and 45% decrease, respectively) and morphine (34% and 45%, respectively). The antiapoptotic effect of morphine was abolished by naloxone (10 nM), a nonselective opioid receptor antagonist, or xestospongin C (XeC, 400 nM), an inhibitor of inositol (1,4,5)-trisphosphate [Ins(1,4,5)P3]-mediated Ca2+ release. Ca2+ preconditioning, induced by increasing extracellular Ca2+ from 1.8 to 3.3 mM, mimicked the antiapoptotic effect of morphine on DNA fragmentation (24% decrease) and cytochrome c release (57% decrease). This effect mediated by extracellular Ca2+ was also abolished by XeC. Measurements of intracellular Ca2+ concentration using fura-2 microspectrofluorimetry showed that morphine induces Ins(1,4,5)P3-dependent Ca2+ transients abolished by 2-aminoethoxydiphenyl borate (2-APB), a cell-permeable Ins(1,4,5)P3 antagonist. These results suggest that morphine preconditioning prevents simulated ischemia-reperfusion-induced apoptosis via an Ins(1,4,5)P3 signaling pathway in rat ventricular myocytes.

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