Molecular characterization and functional analysis of daf-8 in the pinewood nematode, Bursaphelenchus xylophilus

Author(s):  
Jinghan Wang ◽  
Huan Hong ◽  
Rong Xie ◽  
Jingjing Ji ◽  
Kai Guo ◽  
...  
2021 ◽  
Vol 51 (1) ◽  
Author(s):  
Lifeng Zhou ◽  
Jingjing Ji ◽  
Najie Zhu ◽  
Kai Guo ◽  
Jia Tang ◽  
...  

2015 ◽  
Vol 45 (6) ◽  
pp. 467-473 ◽  
Author(s):  
J. M. S. Cardoso ◽  
L. Fonseca ◽  
P. Gomes ◽  
C. Egas ◽  
I. Abrantes

Forests ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 265
Author(s):  
Joana M.S. Cardoso ◽  
Luís Fonseca ◽  
Isabel Abrantes

The pinewood nematode (PWN) Bursaphelenchus xylophilus, the causal agent of the pine wilt disease (PWD), enters above-ground parts of the tree, migrates through the resin canals and feeds on plant cells causing extensive damage. In order to penetrate the cell wall and establish a parasitic relationship with host trees, the PWN needs to secretea mixture of active cell wall degrading enzymes. In maritime pine, Pinus pinaster, which is high susceptible to PWN, xyloglucan is the major hemicellulosic polysaccharide in primary cells. The xyloglucan backbone is susceptible to hydrolysis by numerous endoglucanases, some of them specific to xyloglucan. However, to completely degrade xyloglucan, all substitutions on the glucan backbones must be released, and l-fucose residues in xyloglucan branches are released by α-l-fucosidases. In the present study, the molecular characterization of two α-l-fucosidases found in PWN secretome was performed. Moreover, a novel α-l-fucosidase was identified and its cDNA and gene sequence were determined. The three-dimensional structures of these α-l-fucosidases were predicted and the transcript levels were analyzed, thus providing new insights into fundamental PWN biology and the possible role of these proteins as cell wall degrading enzymes.


2019 ◽  
Vol 49 (2) ◽  
pp. e12467 ◽  
Author(s):  
Buyong Wang ◽  
Rongrong Wen ◽  
Ling Ma ◽  
Yan Ma ◽  
T. Hua Zhou

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
P Barbosa ◽  
AM Rodrigues ◽  
JMS Faria ◽  
LG Pedro ◽  
JG Barroso ◽  
...  

2017 ◽  
Vol 43 (2) ◽  
pp. 201
Author(s):  
Pei ZHAO ◽  
Li-Jie TENG ◽  
Ke WANG ◽  
Li-Pu DU ◽  
Xian REN ◽  
...  

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