Aspartic peptidases of the pinewood nematode Bursaphelenchus xylophilus : Molecular characterization and in silico structural analysis

2019 ◽  
Vol 49 (5) ◽  
pp. e12545
Author(s):  
Joana M. S. Cardoso ◽  
Luís Fonseca ◽  
Isabel Abrantes
Keyword(s):  
Structural Analysis ◽  
Molecular Characterization ◽  
In Silico ◽  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode ◽  
Aspartic Peptidases

scholarly journals α-l-Fucosidases from Bursaphelenchus xylophilus Secretome—Molecular Characterization and Their Possible Role in Breaking Down Plant Cell Walls

Forests ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 265
Author(s):  
Joana M.S. Cardoso ◽  
Luís Fonseca ◽  
Isabel Abrantes
Keyword(s):  
Cell Wall ◽  
Molecular Characterization ◽  
Three Dimensional ◽  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode ◽  
Cell Wall Degrading Enzymes ◽  
Degrading Enzymes ◽  
Host Trees ◽  
Resin Canals

The pinewood nematode (PWN) Bursaphelenchus xylophilus, the causal agent of the pine wilt disease (PWD), enters above-ground parts of the tree, migrates through the resin canals and feeds on plant cells causing extensive damage. In order to penetrate the cell wall and establish a parasitic relationship with host trees, the PWN needs to secretea mixture of active cell wall degrading enzymes. In maritime pine, Pinus pinaster, which is high susceptible to PWN, xyloglucan is the major hemicellulosic polysaccharide in primary cells. The xyloglucan backbone is susceptible to hydrolysis by numerous endoglucanases, some of them specific to xyloglucan. However, to completely degrade xyloglucan, all substitutions on the glucan backbones must be released, and l-fucose residues in xyloglucan branches are released by α-l-fucosidases. In the present study, the molecular characterization of two α-l-fucosidases found in PWN secretome was performed. Moreover, a novel α-l-fucosidase was identified and its cDNA and gene sequence were determined. The three-dimensional structures of these α-l-fucosidases were predicted and the transcript levels were analyzed, thus providing new insights into fundamental PWN biology and the possible role of these proteins as cell wall degrading enzymes.

Identification, Molecular Characterization, and In Silico Structural Analysis of Carboxypeptidase B2 ofAnopheles stephensi

2018 ◽  
Vol 56 (1) ◽  
pp. 72-85 ◽  
Author(s):  
Mahdokht Ilbeigi khamsehnejad ◽  
Navid Dinparast Djadid ◽  
Abbasali Raz
Keyword(s):  
Structural Analysis ◽  
Molecular Characterization ◽  
In Silico

Molecular characterization and functional analysis of akt‐1 in pinewood nematode, Bursaphelenchus xylophilus

2021 ◽  
Vol 51 (1) ◽  
Author(s):  
Lifeng Zhou ◽  
Jingjing Ji ◽  
Najie Zhu ◽  
Kai Guo ◽  
Jia Tang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Characterization ◽  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode

Cysteine proteases secreted by the pinewood nematode, Bursaphelenchus xylophilus: In silico analysis

2018 ◽  
Vol 77 ◽  
pp. 291-296 ◽  
Author(s):  
Joana M.S. Cardoso ◽  
Luís Fonseca ◽  
Conceição Egas ◽  
Isabel Abrantes
Keyword(s):  
In Silico ◽  
In Silico Analysis ◽  
Cysteine Proteases ◽  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode ◽  
Silico Analysis

Nematotoxic activity from essential oils and their hydrocarbons and oxygen-containing molecules fractions against the pinewood nematode Bursaphelenchus xylophilus

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
P Barbosa ◽  
AM Rodrigues ◽  
JMS Faria ◽  
LG Pedro ◽  
JG Barroso ◽  
...  
Keyword(s):  
Essential Oils ◽  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode

scholarly journals Bxy‐fuca encoding α‐L‐fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus

2019 ◽  
Vol 76 (1) ◽  
pp. 205-214 ◽  
Author(s):  
Jia Tang ◽  
Ruoqing Ma ◽  
Najie Zhu ◽  
Kai Guo ◽  
Yiqing Guo ◽  
...  
Keyword(s):  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode

scholarly journals In Silico Structural Modeling and Analysis of Interactions of Tremellomycetes Cytochrome P450 Monooxygenases CYP51s with Substrates and Azoles

2021 ◽  
Vol 22 (15) ◽  
pp. 7811
Author(s):  
Olufunmilayo Olukemi Akapo ◽  
Joanna M. Macnar ◽  
Justyna D. Kryś ◽  
Puleng Rosinah Syed ◽  
Khajamohiddin Syed ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Structural Analysis ◽  
In Silico ◽  
Web Application ◽  
Cytochrome P450 Monooxygenases ◽  
Agglomerative Clustering ◽  
Ligand Complex ◽  
P450 Monooxygenase ◽  
Study Results

Cytochrome P450 monooxygenase CYP51 (sterol 14α-demethylase) is a well-known target of the azole drug fluconazole for treating cryptococcosis, a life-threatening fungal infection in immune-compromised patients in poor countries. Studies indicate that mutations in CYP51 confer fluconazole resistance on cryptococcal species. Despite the importance of CYP51 in these species, few studies on the structural analysis of CYP51 and its interactions with different azole drugs have been reported. We therefore performed in silico structural analysis of 11 CYP51s from cryptococcal species and other Tremellomycetes. Interactions of 11 CYP51s with nine ligands (three substrates and six azoles) performed by Rosetta docking using 10,000 combinations for each of the CYP51-ligand complex (11 CYP51s × 9 ligands = 99 complexes) and hierarchical agglomerative clustering were used for selecting the complexes. A web application for visualization of CYP51s’ interactions with ligands was developed (http://bioshell.pl/azoledocking/). The study results indicated that Tremellomycetes CYP51s have a high preference for itraconazole, corroborating the in vitro effectiveness of itraconazole compared to fluconazole. Amino acids interacting with different ligands were found to be conserved across CYP51s, indicating that the procedure employed in this study is accurate and can be automated for studying P450-ligand interactions to cater for the growing number of P450s.

scholarly journals Genetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Qiaoli Chen ◽  
Ruizhi Zhang ◽  
Danlei Li ◽  
Feng Wang
Keyword(s):  
Fat Metabolism ◽  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode ◽  
Genetic Characteristics ◽  
Highest Weight ◽  
Homologous Genes ◽  
Third Stage ◽  
Highly Correlated ◽  
Oil Red O Staining ◽  
Oil Red O

AbstractThe third-stage dispersal juvenile (DJ3) of pinewood nematode (PWN) is highly associated with low-temperature survival and spread of the nematode. Oil-Red-O staining showed that its lipid content was significantly higher compared with other PWN stages. Weighted gene coexpression network analysis identified that genes in the pink module were highly related to DJ3 induced in the laboratory (DJ3-lab). These genes were arranged according to their gene significance (GS) to DJ3-lab. Of the top 30 genes with the highest GS, seven were found to be highly homologous to the cysteine protease family cathepsin 1 (CATH1). The top 30 genes with the highest weight value to each of the seven genes in the pink module were selected, and finally 35 genes were obtained. Between these seven CATH1 homologous genes and their 35 highly related genes, 15 were related to fat metabolism or autophagy. These autophagy-related genes were also found to be highly correlated with other genes in the pink module, suggesting that autophagy might be involved in the mechanism of longevity in DJ3 and the formation of DJ3 by regulating genes related to fat metabolism.

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