Optimization of ultrasonic-assisted extraction, refinement and characterization of water-soluble polysaccharide from Dictyosphaerium sp. and evaluation of antioxidant activity in vitro

2019 ◽  
Vol 14 (2) ◽  
pp. 963-977 ◽  
Author(s):  
Chen Chen ◽  
Zhenyu Zhao ◽  
Shasha Ma ◽  
Muhammad Azam Rasool ◽  
Le Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Water Soluble ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Soluble Polysaccharide ◽  
Ultrasonic Assisted Extraction

scholarly journals Ultrasonic-assisted Extraction, Antioxidant Activity and Structural Characterization of Polysaccharides from Oenothera Biennis L. Leaves

2020 ◽  
Vol 189 ◽  
pp. 02025
Author(s):  
Zhou Yong Dong ◽  
Xin Song Yin ◽  
Shu Lin Liu ◽  
Tie Hua Zhang ◽  
Hui Ren ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Average Molecular Weight ◽  
Reducing Power ◽  
Ultrasonic Power ◽  
Oenothera Biennis ◽  
Scavenging Effect ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

This paper studies the optimization of ultrasonic-assisted extraction of polysaccharides from Oenothera biennis L. leaves using Box-Behnken design, and their in vitro antioxidant activity and structure. It is demonstrated that the optimum extraction conditions are: the ultrasonic power at 400 W, solvent-tosample ratio of 23:1 (mL/g), ultrasonication temperature at 53 °C, and time for 36 min, under which the yield of polysaccharides from Oenothera biennis L. leaves, namely 3.54%, remains close to the predicted value in the model. The assays of the scavenging effect on DPPH and hydroxyl free radicals, and of the reducing power indicate that polysaccharides from Oenothera biennis L. leaves possess strong antioxidant activity and certain relationship exists between their dose and effect in the concentration range of the experiment; Fourier IR spectroscopy reveals that characteristic absorption peak of polysaccharides can be seen from the sample; the DE-52 cellulose column chromatography, HPLC profiles, and GC-MS chromatogram identify the obtained OCP-2 as heteropolysaccharides, whose major components, including arabinose, mannose, galactose, and talose, have an average molecular weight of 5435 Da.

scholarly journals Flavonoids Extraction fromTaraxacum officinale(Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Zongxi Sun ◽  
Ruiqiang Su ◽  
Jianwei Qiao ◽  
Zhiquan Zhao ◽  
Xinsheng Wang
Keyword(s):  
Antioxidant Activity ◽  
Response Surface ◽  
Extraction Yield ◽  
Solid Ratio ◽  
Plant Resource ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Macroporous Adsorption Resin ◽  
Ultrasonic Assisted Extraction

The Box-Behnken design combined with response surface method was employed to optimize ultrasonic-assisted extraction of flavonoids fromTaraxacum officinale. The optimized results showed that the highest extraction yield with ultrasonic-assisted extraction could reach 2.62% using 39.6% (v/v) ethanol and 59.5 : 1 (mL/g) liquid-solid ratio for 43.8 min. The crude extract was then purified by HPD-100 macroporous adsorption resin, and the flavonoids content in the purified extract increased to 54.7%. The antioxidant activity of the purified flavonoids was evaluatedin vitroby scavenging capacity of ABTS or DPPH,β-carotene bleaching, and FTC test. The knowledge obtained from this study should be useful to further develop and apply this plant resource.

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