Antimicrobial effect of Eleutherine americana bulb extract on the growth of Campylobacter jejuni in broiler meat

Survival of Campylobacter jejuni and Campylobacter coli isolated from broiler meat was investigated and modeled on retail breast meat. Meat portions were inoculated with C. jejuni or C. coli at 6.4 to 6.8 log CFU/g followed by storage at −20°C for 84 days or at 4 or 12°C for 14 days. Kinetic data within a species and temperature were fitted to the Weibull model. When ≥70% of the residuals were in an acceptable prediction zone from −1 (fail-safe) to 0.5 (fail-dangerous) log units, the model was considered to have acceptable performance. Survival of Campylobacter was highest at 4°C, lowest at 12°C, and intermediate at −20°C. Survival of C. jejuni and C. coli was similar at −20°C but was lower (P < 0.05) for C. jejuni than for C. coli at 4 and 12°C. The Weibull model provided acceptable predictions for four of six sets of dependent data with unacceptable performance for survival of C. jejuni at −20 and 12°C. A difference in survival was observed between the two strains of C. jejuni tested. Comparison of Weibull model predictions with data for C. jejuni archived in ComBase revealed mostly unacceptable performance, indicating that C. jejuni and C. coli survival on raw broiler breast meat differs from published results for other strains and growth media. Variation in Campylobacter survival among replicate storage trials was high, indicating that performance of the models can be improved by collection of additional data to better define the survival response during storage at temperatures from −20 to 12°C.

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Assessment of in Vivo Antimicrobials Effect of some Medicinal Plants Seeds on Broilers Meat Keeping Quality

Academic Journal of Life Sciences ◽

10.32861/ajls.62.8.12 ◽

2020 ◽

pp. 8-12

Author(s):

Mohamed Elkhatim ◽

Abdelmageed Hyder O. Abdalla ◽

Omer H. Arabi ◽

Babiker M. S. ◽

Abdel Moneim E. Sulieman

Keyword(s):

Antimicrobial Effect ◽

Storage Conditions ◽

Dilution Method ◽

Broiler Meat ◽

Antimicrobial Effects ◽

Black Cumin ◽

Significant Difference ◽

Keeping Quality

The aim of the study is to evaluate the in vivo antimicrobial effect of some natural products, that include seeds of: Black cumin Fenugreek, Moringa olefera, Clitoria ternatea, Sunflower and Vit E on broiler meat keeping quality. One hundred and five one day old male chicks were used. They were allotted to 7 treatments with 3 replications each in CRD design .The chicks were fed balanced rations supplemented with tested seeds and Vit E. In vivo antimicrobial effects of tested seeds were measured using the serial dilution method on chickens breast samples at fresh, - 4oC and at – 20oC for 30 days. The tested seeds had an in vitro antifungal and antimicrobial effect on Gram + ve and Gram -ve bacteria. The in vivo antimicrobial effects of tested seeds showed no significant at fresh and -20oC storage conditions; however, there was a significant difference among treatments at – 4oC-storage condition. Nevertheless, there was a significant difference among storage conditions. Generally, the results demonstrated that the tested seeds can be used as an in vitro antimicrobial natural source.

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