Use of ultraviolet irradiation to reduce Campylobacter jejuni on broiler meat

Survival of Campylobacter jejuni and Campylobacter coli isolated from broiler meat was investigated and modeled on retail breast meat. Meat portions were inoculated with C. jejuni or C. coli at 6.4 to 6.8 log CFU/g followed by storage at −20°C for 84 days or at 4 or 12°C for 14 days. Kinetic data within a species and temperature were fitted to the Weibull model. When ≥70% of the residuals were in an acceptable prediction zone from −1 (fail-safe) to 0.5 (fail-dangerous) log units, the model was considered to have acceptable performance. Survival of Campylobacter was highest at 4°C, lowest at 12°C, and intermediate at −20°C. Survival of C. jejuni and C. coli was similar at −20°C but was lower (P < 0.05) for C. jejuni than for C. coli at 4 and 12°C. The Weibull model provided acceptable predictions for four of six sets of dependent data with unacceptable performance for survival of C. jejuni at −20 and 12°C. A difference in survival was observed between the two strains of C. jejuni tested. Comparison of Weibull model predictions with data for C. jejuni archived in ComBase revealed mostly unacceptable performance, indicating that C. jejuni and C. coli survival on raw broiler breast meat differs from published results for other strains and growth media. Variation in Campylobacter survival among replicate storage trials was high, indicating that performance of the models can be improved by collection of additional data to better define the survival response during storage at temperatures from −20 to 12°C.

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Multilocus Sequence Typing (MLST) of Campylobacter jejuni Isolated From Broiler Meat in Egypt

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2019.574.579 ◽

2019 ◽

Vol 22 (12) ◽

pp. 574-579

Author(s):

Amal Awad ◽

Rasha Elkenany ◽

Asmaa Sadat ◽

Wafaa Ragab ◽

Mohamed Elhadidy

Keyword(s):

Campylobacter Jejuni ◽

Multilocus Sequence Typing ◽

Broiler Meat

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Bacteriophage Therapy to Reduce Colonization of Campylobacter jejuni in Broiler Chickens before Slaughter

Viruses ◽

10.3390/v13081428 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1428

Author(s):

Daniela D’Angelantonio ◽

Silvia Scattolini ◽

Arianna Boni ◽

Diana Neri ◽

Gabriella Di Serafino ◽

...

Keyword(s):

Campylobacter Jejuni ◽

Broiler Chickens ◽

Phage Therapy ◽

Gastrointestinal Disease ◽

Bacterial Colonization ◽

Resistance Pattern ◽

Control Group ◽

Bacteriophage Therapy ◽

Broiler Meat ◽

Group B

Campylobacteriosis is the most commonly reported gastrointestinal disease in humans. Campybacter jejuni is the main cause of the infection, and bacterial colonization in broiler chickens is widespread and difficult to prevent, leading to high risk of occurrence in broiler meat. Phage therapy represents an alternative strategy to control Campylobacter in poultry. The aim of this work was to assess the efficacy of two field-isolated bacteriophages against experimental infections with an anti-microbial resistant (AMR) Campylobacter jejuni strain. A two-step phage application was tested according to a specific combination between chickens’ rearing time and specific multiplicities of infections (MOIs), in order to reduce the Campylobacter load in the animals at slaughtering and to limit the development of phage-resistant mutants. In particular, 75 broilers were divided into three groups (A, B and C), and phages were administered to animals of groups B and C at day 38 (Φ 16-izsam) and 39 (Φ 7-izsam) at MOI 0.1 (group B) and 1 (group C). All broilers were euthanized at day 40, and Campylobacter jejuni was enumerated in cecal contents. Reductions in Campylobacter counts were statistically significant in both group B (1 log10 colony forming units (cfu)/gram (gr)) and group C (2 log10 cfu/gr), compared to the control group. Our findings provide evidence about the ability of phage therapy to reduce the Campylobacter load in poultry before slaughtering, also associated with anti-microbial resistance pattern.

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Risk analysis of Campylobacter jejuni in broiler meat food chain

10.2298/bg20131115petkovic ◽

2013 ◽

Author(s):

Jelena Petkovic

Keyword(s):

Risk Analysis ◽

Campylobacter Jejuni ◽

Food Chain ◽

Broiler Meat

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Ultrastructure of periplasmic bodies in gram-negative bacteria

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160753 ◽

1990 ◽

Vol 48 (3) ◽

pp. 640-641

Author(s):

Xie Nianming ◽

Ding Shaoqing ◽

Wang Luping ◽

Yuan Zenglin ◽

Zhan Guolai ◽

...

Keyword(s):

Electron Microscope ◽

Campylobacter Jejuni ◽

Proteus Mirabilis ◽

Shigella Flexneri ◽

Morphological Description ◽

Gram Negative Bacteria ◽

Periplasmic Space ◽

Clostridium Tetani ◽

Gram Negative ◽

Brucella Canis

Perhaps the data about periplasmic enzymes are obtained through biochemical methods but lack of morphological description. We have proved the existence of periplasmic bodies by electron microscope and described their ultrastructures. We hope this report may draw the attention of biochemists and mrophologists to collaborate on researches in periplasmic enzymes or periplasmic bodies with each other.One or more independent bodies may be seen in the periplasmic space between outer and inner membranes of Gram-negative bacteria, which we called periplasmic bodies. The periplasmic bodies have been found in seven species of bacteria at least, including the Pseudomonas aeroginosa. Shigella flexneri, Echerichia coli. Yersinia pestis, Campylobacter jejuni, Proteus mirabilis, Clostridium tetani. Vibrio cholerae and Brucella canis.

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