Design of aptamer-based sensing platform using gold nanoparticles functionalized reduced graphene oxide for ultrasensitive detection of Hepatitis B virus

2020 ◽  
Vol 75 (1) ◽  
pp. 279-295
Author(s):  
Dheyaa Hussein Mohsin ◽  
Muthana Saleh Mashkour ◽  
Fataneh Fatemi
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reduced Graphene Oxide ◽  
Ultrasensitive Detection ◽  
Sensing Platform ◽  
Reduced Graphene ◽  
B Virus

IMMOBILIZATION OF HEPATITIS B ANTIBODY ONTO THE REDUCED GRAPHENE OXIDE

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
G. S. C. Chan ◽  
W. X. F. Wong ◽  
F. C. Chong
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reduced Graphene Oxide ◽  
Surface Antigen ◽  
Virus Surface ◽  
Reduced Graphene ◽  
B Virus ◽  
Secondary Antibodies ◽  
Hep B

Hepatitis B (Hep B) is a viral infection caused by hepatitis B virus (HBV) that infects the human liver. HBV is blood-borne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide. Approximately 5% of the Malaysian population is infected by HBV. The treatment to this disease is possible if early detection is done before irreversible liver damage occurs. The presence of hepatitis B virus surface antigen (HBsAg) is an evidence of the presence of HBV infections. Presently, the conventional methods used for detection show weaknesses in terms of time and efficiency. In this research, the functionality of reduced graphene oxide (rGO) as an immuno biosensor for the detection of HBsAg has been studied. The graphene oxide was prepared via Modified Hummer’s Method due to its ability to produce graphene in large quantity. The fabrication of immunosensor was conducted by immobilizing antibody onto the graphene. nafion-graphene nanosheet was grown and later treated with thionine solution. Following that, a solution of primary antibodies against Hep B surface antigen (anti-HBsAg IgG) was incubated on the biosensor. After the immobilization, the antigen was dropped onto the biosensor, followed by incubation of secondary antibodies conjugated to Horseradish Peroxidase (HRP). Upon addition of Tetramethylbenzidine (TMB), the colour of the solution changes to blue. Thus, proving the functionality of rGO as an immunobiosensor for the detection of HBsAg.

scholarly journals Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen

2018 ◽  
Vol 122 ◽  
pp. 199-205 ◽  
Author(s):  
Mohamad Farid Abd Muain ◽  
Kooi Hoong Cheo ◽  
Muhamad Nadzmi Omar ◽  
Amir Syahir Amir Hamzah ◽  
Hong Ngee Lim ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Gold Nanoparticle ◽  
Reduced Graphene Oxide ◽  
Core Antigen ◽  
Virus Core ◽  
Reduced Graphene ◽  
B Virus

Reduced graphene oxide-wrapped silver nanoparticles for applications in ultrasensitive colorimetric detection of Cr(vi) ions and the carbaryl pesticide

2020 ◽  
Vol 44 (18) ◽  
pp. 7611-7620
Author(s):  
Phung Nhat Minh ◽  
Van-Tuan Hoang ◽  
Ngo Xuan Dinh ◽  
Ong Van Hoang ◽  
Nguyen Van Cuong ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Colorimetric Detection ◽  
Ultrasensitive Detection ◽  
Colorimetric Sensing ◽  
Sensing Platform ◽  
Reduced Graphene

The Ag@rGO nanohybrid can be used as a colorimetric sensing platform for ultrasensitive detection of Cr(vi) ions and the carbaryl pesticide.

An impedimetric aptasensor for ultrasensitive detection of Penicillin G based on the use of reduced graphene oxide and gold nanoparticles

2019 ◽  
Vol 186 (6) ◽  
Author(s):  
Ayat Mohammad-Razdari ◽  
Mahdi Ghasemi-Varnamkhasti ◽  
Zahra Izadi ◽  
Ali A. Ensafi ◽  
Sajad Rostami ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Penicillin G ◽  
Ultrasensitive Detection ◽  
Reduced Graphene

Label-free electrochemical immunosensor based on gold nanoparticle/polyethyleneimine/reduced graphene oxide nanocomposite for the ultrasensitive detection of cancer biomarker matrix metalloproteinase-1

The Analyst ◽  
2021 ◽  
Author(s):  
Xinke Liu ◽  
Lu-Yin Lin ◽  
Fu-Yen Tseng ◽  
Yu-Cheng Tan ◽  
Jian Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Matrix Metalloproteinase ◽  
Gold Nanoparticle ◽  
Reduced Graphene Oxide ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free ◽  
Matrix Metalloproteinase 1 ◽  
Reduced Graphene ◽  
P Matrix

Matrix metalloproteinase-1 (MMP-1) is associated with many types of cancers, including oral, colorectal, and brain cancers. This paper describes the fabrication of an MMP-1 immunosensor based on a gold nanoparticle/polyethyleneimine/reduced...

Sign in / Sign up

Export Citation Format

Share Document