Label-free electrochemical immunosensor based on gold nanoparticle/polyethyleneimine/reduced graphene oxide nanocomposite for the ultrasensitive detection of cancer biomarker matrix metalloproteinase-1

The Analyst ◽  
2021 ◽  
Author(s):  
Xinke Liu ◽  
Lu-Yin Lin ◽  
Fu-Yen Tseng ◽  
Yu-Cheng Tan ◽  
Jian Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Matrix Metalloproteinase ◽  
Gold Nanoparticle ◽  
Reduced Graphene Oxide ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free ◽  
Matrix Metalloproteinase 1 ◽  
Reduced Graphene ◽  
P Matrix

Matrix metalloproteinase-1 (MMP-1) is associated with many types of cancers, including oral, colorectal, and brain cancers. This paper describes the fabrication of an MMP-1 immunosensor based on a gold nanoparticle/polyethyleneimine/reduced...

scholarly journals A Bifunctional Nanosilver-Reduced Graphene Oxide Nanocomposite for Label-Free Electrochemical Immunosensing

2021 ◽  
Vol 9 ◽  
Author(s):  
Supakeit Chanarsa ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
One Pot ◽  
Reduced Graphene ◽  
Sensitivity And Selectivity ◽  
Analytical Response ◽  
Electrochemical Immunosensing

A bi-functional material based on silver nanoparticles (AgNPs)-reduced graphene oxide (rGO) composite for both electrode modification and signal generation is successfully synthesized for use in the construction of a label-free electrochemical immunosensor. An AgNPs/rGO nanocomposite is prepared by a one-pot wet chemical process. The AgNPs/rGO composite dispersion is simply cast on a screen-printed carbon electrode (SPCE) to fabricate the electrochemical immunosensor. It possesses a sufficient conductivity/electroreactivity and improves the electrode reactivity of SPCE. Moreover, the material can generate an analytical response due to the formation of immunocomplexes for detection of human immunoglobulin G (IgG), a model biomarker. Based on electrochemical stripping of AgNPs, the material reveals signal amplification without external redox molecules/probes. Under optimized conditions, the square wave voltammetric peak current is responded to the logarithm of IgG concentration in two wide linear ranges from 1 to 50 pg.ml−1 and 0.05 to 50 ng.ml−1, and the limit of detection (LOD) is estimated to be 0.86 pg.ml−1. The proposed immunosensor displays satisfactory sensitivity and selectivity. Importantly, detection of IgG in human serum using the immunosensor shows satisfactory accuracy, suggesting that the immunosensor possesses a huge potential for further development in clinical diagnosis.

A novel label-free electrochemical immunosensor for the detection of hepatitis B surface antigen

2016 ◽  
Vol 8 (40) ◽  
pp. 7380-7386 ◽  
Author(s):  
Lihui Tian ◽  
Li Liu ◽  
Yueyuan Li ◽  
Xue Feng ◽  
Qin Wei ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B ◽  
Reduced Graphene Oxide ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Electrochemical Immunosensor ◽  
Quantitative Detection ◽  
Label Free ◽  
Graphene Oxide Nanosheets ◽  
Reduced Graphene

A novel label-free electrochemical immunosensor based on trimetallic NiAuPt nanoparticles on reduced graphene oxide nanosheets (NiAuPt–NGs) was developed for the quantitative detection of hepatitis B surface antigen (HBsAg).

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