In vivo and in vitro effects of copper and cadmium on the plasma membrane H+-ATPase from cucumber (Cucumis sativus L.) and maize (Zea mays L.) roots

2003 ◽  
Vol 25 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Marek Burzyński ◽  
Ewa Kolano
Keyword(s):  
Zea Mays ◽  
Plasma Membrane ◽  
Cucumis Sativus ◽  
Zea Mays L ◽  
Cucumis Sativus L ◽  
In Vitro Effects

scholarly journals Development of a New Maize (Zea mays L.) Breeding Program

2002 ◽  
pp. 25-30
Author(s):  
Pál Pepó ◽  
Szilárd Tóth
Keyword(s):  
Zea Mays ◽  
Recurrent Selection ◽  
Zea Mays L ◽  
Genetic Manipulation ◽  
Dominant Gene ◽  
Breeding Program ◽  
Regeneration Ability ◽  
Breeding Programs

Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.

scholarly journals Evaluation of the in vitro and in vivo antihypertensive effect and antioxidant activity of blue corn hydrolysates derived from wet-milling//Evaluación del efecto antihipertensivo in vitro e in vivo y actividad antioxidante del hidrolizado de maíz azul derivado de la molienda húmeda

Biotecnia ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Alvaro Montoya-Rodríguez ◽  
Evelyn Isabel Osuna-Gallardo ◽  
Francisco Cabrera-Chávez ◽  
Jorge Milán-Carrillo ◽  
Cuauhtémoc Reyes-Moreno ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Antioxidant Activity ◽  
Zea Mays ◽  
Antihypertensive Effect ◽  
Zea Mays L ◽  
Enzymatic Digestion ◽  
Wet Milling ◽  
Food Ingredient

Hypertension is considered a risk factor for coronary heart disease, and its prevalence has increased substantially. Inhibition of angiotensin-converting enzyme (ACE-I) is key to lower blood pressure, making it an excellent treatment for hypertension. Corn (Zea mays L.) is an important source of bioactive peptides with potential anti-hypertensive activity related to ACE-I inhibition. These peptides can be obtained through the hydrolysis of corn gluten meal (CGM), as wetmilling by-products. The aim was to evaluate the in vitro and in vivo ACE-I inhibitory activity of blue CGM hydrolysates. Enzymatic digestion in vitro of blue CGM was conducted at different times. Hydrolysis for 360 min significantly increased both soluble protein and antioxidant activity by 4 and 8-fold respectively, the maximum ACE-I inhibition (94.3 %) was observed with 260 min hydrolysate. Mice were treated with the blue CGM hydrolysate (260 min), captopril or PBS to test the bioavailability in vivo. The CGM hydrolysate was detected in serum after 5 and up to 30 min after ingestion, showing the maximum ACE-I inhibitory capacity (59 %) during the first 15 min. Overall, this work showed that the blue CGM hydrolysate could serve as a functional food ingredient with antihypertensive effect due to its blood pressure-lowering peptides.RESUMENLa hipertensión es factor de riesgo en enfermedades coronarias, y su prevalencia ha aumentado sustancialmente. La inhibición de enzima convertidora de angiotensina (ECA) es clave para disminuir presión arterial, y excelente tratamiento para hipertensión. El maíz (Zea mays L.) es fuente de péptidos bioactivos con actividad antihipertensiva por inhibición de ECA. Péptidos pueden obtenerse por hidrólisis de harina de gluten de maíz (HGM), como subproducto de molienda húmeda. El objetivo fue evaluar in vitro e in vivo actividad inhibitoria de ECA en hidrolizados de HGM azul. La digestión enzimática in vitro de HGM fue conducida a diferentes tiempos. La hidrólisis por 360 min aumento significativamente proteína soluble y actividad antioxidante de 4 y 8 veces, respectivamente; la máxima inhibición de ECA (94.3 %) fue observada a 260 minutos del hidrolizado. Ratones fueron tratados con HGM hidrolizado (260 minutos), captopril o PBS para evaluar biodisponibilidad in vivo. Después de la ingestión, HGM hidrolizado fue detectado en suero en 5 hasta 30 minutos, mostrando máxima inhibición de ECA (59 %) durante los primeros 15 minutos. En general, este trabajo mostró que hidrolizado de HGM podría servir como ingrediente funcional en alimentos con efecto antihipertensivo debido a péptidos reductores de presión arterial.

In vivo and in vitro effects of boron on the plasma membrane proton pump of sunflower roots

1992 ◽  
Vol 84 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Marta Roldan ◽  
Andres Belver ◽  
Pilar Rodriguez-Rosales ◽  
Nuria Ferrol ◽  
Juan Pedro Donaire
Keyword(s):  
Plasma Membrane ◽  
Proton Pump ◽  
In Vitro Effects ◽  
Plasma Membrane Proton Pump

Safeners as Corn Seedling Protectants against Acetolactate Synthase Inhibitors

1991 ◽  
Vol 46 (11-12) ◽  
pp. 945-949
Author(s):  
Henri Milhomme ◽  
Christophe Roux ◽  
Jean Bastide
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Acetolactate Synthase ◽  
Large Degree ◽  
Naphthalic Anhydride ◽  
Metsulfuron Methyl ◽  
Corn Seedling

Zea mays L., cv. Potro, shoots had a higher level of resistance to imazaquin (IQ) and metsulfuron methyl (M SM ) than roots. Shoot lengths were increased by pretreating the seeds with commercial 1,8 -naphthalic anhydride (N A ) at 1% (w/w) or oxabetrinil at 0.2% (w/w). The growth of shoots of safened seeds was unaffected by 400 nM IQ and by 40 nM MSM. The in vivo activity of acetolactate synthase (ALS) extracted from corn shoots and roots was not affected by treatments with IQ or MSM, but pretreatment of seeds with NA or oxabetrinil, prior to germination, caused an increase in the level of extractable ALS from shoots. ALS activity from roots and shoots of NA-pretreated seedlings was increased to a large degree (> 40% ) when the seedlings were germinated on 40 nM MSM, whereas ALS activities from oxabetrinil-pretreated seedlings were enhanced to a lesser degree (≈20%). ALS from unsafened seedlings was inhibited 21% by 400 nM IQ and 70% by 40 nM MSM in vitro, but ALS from roots of seedlings germinated on 400 nM IQ was not inhibited by 400 nM IQ in vitro.

scholarly journals Phytotoxicity of ZnO nanoparticles and the released Zn(II) ion to corn (Zea mays L.) and cucumber (Cucumis sativus L.) during germination

2015 ◽  
Vol 22 (14) ◽  
pp. 11109-11117 ◽  
Author(s):  
Ruichang Zhang ◽  
Haibo Zhang ◽  
Chen Tu ◽  
Xuefeng Hu ◽  
Lianzhen Li ◽  
...  
Keyword(s):  
Zea Mays ◽  
Cucumis Sativus ◽  
Zno Nanoparticles ◽  
Zea Mays L ◽  
Cucumis Sativus L

Nitrogen-metabolizing enzymes of Diplodia maydis, a Zea mays L. stalk rot causing fungus

1979 ◽  
Vol 25 (2) ◽  
pp. 167-169
Author(s):  
James B. Bussard ◽  
Russell L. Larson
Keyword(s):  
Zea Mays ◽  
Nitrate Reductase ◽  
Nitrogen Metabolism ◽  
Zea Mays L ◽  
Ammonium Salts ◽  
Higher Plant ◽  
Stalk Rot ◽  
Relationship Of

The nitrogen source available to Diplodia maydis in vivo is reported to affect the severity of stalk rot in maize. Nitrate and (or) ammonium salts were tested for their effect on the type of nitrogen metabolism found in Diplodia maydis in vitro. The level of glutamate dehydrogenase remained essentially constant on either nitrogen salt but nitrate reductase was induced by growth on nitrate salts and was not extractable on ammonium salts. Properties of nitrate reductase reported here are similar to those reported for the higher plant and Neurospora crassa enzymes. The relationship of nitrogen metabolism in Diplodia maydis to Zea mays L. stalk rot is discussed.

The levels of melatonin and its metabolites in conditioned corn (Zea mays L.) and cucumber (Cucumis sativus L.) seeds during storage

2015 ◽  
Vol 37 (6) ◽  
Author(s):  
Izabela Kołodziejczyk ◽  
Marta Bałabusta ◽  
Rafał Szewczyk ◽  
Małgorzata M. Posmyk
Keyword(s):  
Zea Mays ◽  
Cucumis Sativus ◽  
Zea Mays L ◽  
Cucumis Sativus L
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