CUCUMBER (CUCUMIS SATIVUS L.) DIHAPLOID LINE PRODUCTION USING IN VITRO RESCUE OF IN VIVO INDUCED PARTHENOGENIC EMBRYOS

2006 ◽  
pp. 837-844 ◽  
Author(s):  
R. Dolcet-Sanjuan ◽  
E. Claveria ◽  
J. Garcia-Mas
Keyword(s):  
Cucumis Sativus ◽  
Cucumis Sativus L ◽  
Line Production

scholarly journals DH-plant production in culture of unpollinated ovules of cucumber (Cucumis sativus L.)

2019 ◽  
pp. 3-9
Author(s):  
E. A. Domblides ◽  
N. A. Shmykova ◽  
S. N. Belov ◽  
I. B. Korottseva ◽  
A. V. Soldatenko
Keyword(s):  
Cucumis Sativus ◽  
Plant Production ◽  
F1 Hybrids ◽  
Cucumis Sativus L ◽  
Line Production ◽  
High Productivity ◽  
Date Fruit ◽  
Homozygous Line ◽  
Cucurbitaceae Family

Relevance. The development of F1 hybrids distinguishing it from cultivars by high productivity, plant uniformity in ripening date, fruit sizes and quality is the promising trend in breeding program in cucumber (Cucumis sativus L.).The aim of the study was to optimize the gynogenesis induction condition in culture of unpollinated ovules in vitro in order to broad the generation of new breeding forms and to accelerate homozygous line production.Materials and methods. Eight promising cucumber accessions from Laboratory of Cucurbit Breeding and Seed Production (FSBSI FSVC) were taken for the study. The protocol developed in Laboratory of Biotechnology (FSBSI FSVC) for production of doubled haploid in Cucurbitaceae family was used in the experiment. The medium IMC with 30 g/L sucrose and 7g/L agar supplemented with 200 mg/L ampicillin and 0.2 mg/L thidiazuron (TDZ) was applied to induce gynogenic development.Results. The half-open bud or flower was shown to be the most suitable to be taken as an explant for cultivation. Highest number of embryo-like structures in all accessions developed from ovaries 2.1-2.6 cm long. Exposure to sterilization solution of sodium hypochlorite for 15 min made ovary wall softer and ovules can be then easily extracted without traumatizing. The traumatized ovule resulted in inhibited gynogenic development. Embryoids and calli had developed in all studied cucumber accessions, but well-formed plants were only obtained in six accessions. In total 26 plants were produced. The maximum gynogenesis induction equal to 63.1% was achieved in accession 1810. Maximum number of plant produced was twelve in accession 1763, but the greatest plant outcome 7.7% of the ovules with induced gynogenesis was observed in accession 1807. 

scholarly journals Prooxidative and antioxidative properties of cucumber (Cucumis sativus L.) callus in vitro and young in vivo plantlets in response to copper ions

2013 ◽  
Vol 25 (2) ◽  
pp. 141-151 ◽  
Author(s):  
Alexander S. Lukatkin ◽  
Irina D. Michailova ◽  
Jaime A. Teixeira da Silva
Keyword(s):  
Oxidative Stress ◽  
Cucumis Sativus ◽  
Callus Induction ◽  
Copper Ions ◽  
Callus Formation ◽  
Negative Influence ◽  
Cucumis Sativus L ◽  
Biochemical Indices

ABSTRACT The effects of different concentrations of copper ions (Cu2+ in the form of CuSO4 × 5H2O) on in vivo cucumber (Cucumis sativus L. ‘Edinstvo’) seedlings as well as on in vitro hypocotyl-derived callus were considered. Callus induction from hypocotyls was more prolific than from roots or cotyledons. Thus, callus obtained from hypocotyls of 7-day-old cucumber plants was cultured for 5 weeks on Murashige and Skoog medium containing 4 mg dm-3 2,4-D + 1 mg dm-3 BA supplemented with 0.01 mM, 0.1 mM or 1.0 mM of Cu2+. Biochemical indices related to oxidative stress were assessed. Cu2+ at 0.01 mM stimulated callus induction but 1.0 mM Cu2+ negatively affected callus formation and growth. LPO intensity was significantly lower than the control at all concentrations of Cu2+ but significantly higher than the control in plants exposed to 0.01 or 0.1 mM Cu2+. A similar trend was observed for the generation of the superoxide radical in both callus and plantlets. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APOX) activity increased in both callus and plantlets, but the level of increase in these antioxidant enzyme systems depended on the Cu2+ concentration. Cu2+ ions had a stronger (i.e., more negative) influence on oxidative stress in cucumber seedlings than on cucumber callus.

Effect of Ethylene Inhibitors on in vitro Shoot Multiplication and their Impact on Ethylene Production in Cucumber (Cucumis sativus L.)

2006 ◽  
Vol 1 (3) ◽  
pp. 110-117 ◽  
Author(s):  
Vasudevan Ayyappan . ◽  
Selvaraj Natesan . ◽  
Ganapathi Andy . ◽  
Chang won Choi . ◽  
Manickavasagam Marka . ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Ethylene Production ◽  
Shoot Multiplication ◽  
Ethylene Inhibitors ◽  
Cucumis Sativus L

IN VITRO MORPHOGENESIS OF SHOOTS FROM LEAF EXPLANTS OF CUCUMBER (CUCUMIS SATIVUS L.)

2006 ◽  
pp. 155-160 ◽  
Author(s):  
N. Selvarai ◽  
A. Ganapath ◽  
A. Vasudevan ◽  
G. Vengadesan ◽  
S. Kasthuri Rengan
Keyword(s):  
Cucumis Sativus ◽  
Leaf Explants ◽  
In Vitro Morphogenesis ◽  
Cucumis Sativus L

Effect of Hypocotyl Length on Morphogenesis of Explants of Cucumber (Cucumis sativus L.) in vitro

1992 ◽  
Vol 19 (2) ◽  
pp. 165
Author(s):  
RL Gambley ◽  
W Dodd
Keyword(s):  
Cucumis Sativus ◽  
Growth Regulators ◽  
Multiple Shoots ◽  
Root Production ◽  
Concomitant Increase ◽  
Hypocotyl Length ◽  
Rapid Reduction ◽  
Cucumis Sativus L ◽  
Murashige And Skoog Medium

Explants of cucumber seedlings having different lengths of hypocotyl attached were grown axenically on Murashige and Skoog medium supplemented with kinetin (2 mg L-1). Multiple shoots developed from the apical regions of all explants. In this tissue shoots may also develop at the base of the hypocotyl, but this response is strongly dependent upon the length of the hypocotyl. As the length of the hypocotyl increased beyond 5 mm, there was a rapid reduction in basal shoot numbers and a concomitant increase in root production. We suggest that these responses are related not to the ratio or concentration of endogenous growth regulators but to different regions of sensitivity to growth regulators along the hypocotyl.

scholarly journals Optimization of Cucumber Doubled Haploid Line Production Using In Vitro Rescue of In Vivo Induced Parthenogenic Embryos

2005 ◽  
Vol 130 (4) ◽  
pp. 555-560 ◽  
Author(s):  
Elisabet Claveria ◽  
Jordi Garcia-Mas ◽  
Ramon Dolcet-Sanjuan
Keyword(s):  
Flow Cytometry ◽  
Doubled Haploid ◽  
Chromosome Doubling ◽  
Doubled Haploid Line ◽  
High Rate ◽  
Limiting Factors ◽  
Line Production ◽  
Haploid Plants

Homozygous doubled haploid lines (DHLs) from new cucumber (Cucumis sativus L.) accessions could be useful to accelerate breeding for resistant varieties. DHLs have been generated by in vitro rescue of in vivo induced parthenogenic embryos. The protocol developed involves the following: 1) induction of parthenogenic embryos by pollinating with pollen irradiated with a Co60 γ-ray source at 500 Gy; 2) in vitro rescue of putative parthenogenic embryos identified by their morphology and localized using a dissecting scope or X-ray radiography; 3) discrimination of undesirable zygotic individuals from the homozygous plants using cucumber and melon SSR markers; 4) determination of ploidy level from homozygous plants by flow cytometry; 5) in vitro chromosome doubling of haploids; and 6) acclimation and selfing of selected lines. Codominant markers and flow cytometry confirmed the gametophytic origin of plants regenerated by parthenogenesis, since all homozygous lines were haploids. No spontaneous doubled haploid plants were rescued. Chromosome doubling of haploid plants was accomplished by an in vitro treatment with 500 μm colchicine. Rescue of diploid or chimeric plants was shown by flow cytometry, prior to their acclimation and planting in the greenhouse. Selfing of colchicine-treated haploid plants allowed for the perpetuation by seed of homozygous lines. The high rate of seed set, 90% of the lines produced seed, facilitated the recovery of inbred lines. Despite some limiting factors, parthenogenesis is routinely used in a cucumber-breeding program to achieve complete homozygosity in one generation. Breeding for new commercial hybrid cultivars will be accelerated. DHLs are ideal resources for genomic analyses.

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