Evaluation of endopolyploidy patterns in selected Capsicum and Nicotiana species (Solanaceae)

Biologia ◽  
2021 ◽  
Author(s):  
Viera Fráková ◽  
Lukáš Koprivý ◽  
Marianna Paľová ◽  
Vladislav Kolarčik ◽  
Pavol Mártonfi
Keyword(s):  
Nicotiana Species

Systemic infection of some N-gene-carrying Nicotiana species and cultivars after inoculation with tobacco mosaic virus

1977 ◽  
Vol 83 (2) ◽  
pp. 41-59 ◽  
Author(s):  
Jeanne Dijkstra ◽  
G. C. A. Bruin ◽  
Ankie C. Burgers ◽  
L. C. Loon ◽  
Christien Ritter ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Systemic Infection ◽  
N Gene ◽  
Nicotiana Species

scholarly journals Putative Serine Protease Effectors of Clavibacter michiganensis Induce a Hypersensitive Response in the Apoplast of Nicotiana Species

2015 ◽  
Vol 28 (11) ◽  
pp. 1216-1226 ◽  
Author(s):  
You Lu ◽  
Noriyuki Hatsugai ◽  
Fumiaki Katagiri ◽  
Carol A. Ishimaru ◽  
Jane Glazebrook
Keyword(s):  
Nicotiana Tabacum ◽  
Enzymatic Activity ◽  
Hypersensitive Response ◽  
Serine Proteases ◽  
Host Plants ◽  
Ectopic Expression ◽  
Related Protein ◽  
Clavibacter Michiganensis ◽  
Serine Residue ◽  
Nicotiana Species

Clavibacter michiganensis subspp. michiganensis and sepedonicus cause diseases on solanaceous crops. The genomes of both subspecies encode members of the pat-1 family of putative serine proteases known to function in virulence on host plants and induction of hypersensitive responses (HR) on nonhosts. One gene of this family in C. michiganensis subsp. sepedonicus, chp-7, is required for triggering HR in Nicotiana tabacum. Here, further investigation revealed that mutation of the putative catalytic serine residue at position 232 to threonine abolished the HR induction activity of Chp-7, suggesting that enzymatic activity is required. Purified Chp-7 triggered an HR in N. tabacum leaves in the absence of the pathogen, indicating Chp-7 itself is the HR elicitor from C. michiganensis subsp. sepedonicus. Ectopic expression of chp-7 constructs in N. tabacum leaves revealed that Chp-7 targeted to the apoplast triggered an HR while cytoplasmic Chp-7 did not, indicating that Chp-7 induces the HR in the apoplast of N. tabacum leaves. Chp-7 also induced HR in N. sylvestris, a progenitor of N. tabacum, but not in other Nicotiana species tested. ChpG, a related protein from C. michiganensis subsp. michiganensis, also triggered HR in N. tabacum and N. sylvestris. Unlike Chp-7, ChpG triggered HR in N. clevelandii and N. glutinosa.

ADH isozymes in seed of Nicotiana species and somatic hybrids

1984 ◽  
Vol 75 (3) ◽  
pp. 215-219 ◽  
Author(s):  
Stephanie A. Kut ◽  
David A. Evans
Keyword(s):  
Somatic Hybrids ◽  
Nicotiana Species

scholarly journals Tracing the Lineage of Two Traits Associated with the Coat Protein of the Tombusviridae: Silencing Suppression and HR Elicitation in Nicotiana Species

Viruses ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 588 ◽  
Author(s):  
Mustafa Adhab ◽  
Carlos Angel ◽  
Andres Rodriguez ◽  
Mohammad Fereidouni ◽  
Lóránt Király ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Structural Changes ◽  
Coat Proteins ◽  
Silencing Suppressor ◽  
Suppressor Activity ◽  
Nicotiana Species ◽  
Pathogen Effector ◽  
The Family ◽  
Silencing Suppression ◽  
Insight Into

In this paper we have characterized the lineage of two traits associated with the coat proteins (CPs) of the tombusvirids: Silencing suppression and HR elicitation in Nicotiana species. We considered that the tombusvirid CPs might collectively be considered an effector, with the CP of each CP-encoding species comprising a structural variant within the family. Thus, a phylogenetic analysis of the CP could provide insight into the evolution of a pathogen effector. The phylogeny of the CP of tombusvirids indicated that CP representatives of the family could be divided into four clades. In two separate clades the CP triggered a hypersensitive response (HR) in Nicotiana species of section Alatae but did not have silencing suppressor activity. In a third clade the CP had a silencing suppressor activity but did not have the capacity to trigger HR in Nicotiana species. In the fourth clade, the CP did not carry either function. Our analysis illustrates how structural changes that likely occurred in the CP effector of progenitors of the current genera led to either silencing suppressor activity, HR elicitation in select Nicotiana species, or neither trait.

scholarly journals Reaction of Nicotiana species and cultivars of tobacco to Tobacco mosaic virus and detection of the N gene that confers hypersensitive resistance

2018 ◽  
Vol 54 (No. 3) ◽  
pp. 143-146 ◽  
Author(s):  
Depta Anna ◽  
Kursa Karolina ◽  
Doroszewska Teresa ◽  
Laskowska Dorota ◽  
Trojak-Goluch Anna
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Thermal Conditions ◽  
Growth Conditions ◽  
N Gene ◽  
Hypersensitive Resistance ◽  
Nicotiana Species ◽  
Biological Tests ◽  
Temperature Ranges ◽  
Extensive Necrosis

Tobacco mosaic virus (TMV) brings increasing losses in the cultivation of tobacco. Sixty-two cultivars of tobacco and eleven species of Nicotiana were evaluated for resistance to TMV. Biological tests at two temperature ranges, DAS-ELISA and molecular markers were applied to assess the resistance to TMV. Most cultivars of tobacco showed susceptibility (S) to TMV, two were tolerant (T), while others revealed a hypersensitive response (HR). Hypersensitivity, determined by the N gene, occurred only at a temperature below 22°C. At a temperature above 28°C, all the cultivars showed mosaic discolorations or extensive necrosis. The reaction of the Nicotiana species was dependent on growth conditions. At 22°C, the reactions of sensitivity, tolerance and hypersensitivity to TMV were all observed, whereas above 28°C the species showed systemic necrotic symptoms. N. gossei was an exception because hypersensitivity occurred regardless of the thermal conditions. The resistance of this species was not conditioned by the N gene, which suggests that N. gossei could be an additional genetic resource for tobacco breeding.  

Sign in / Sign up

Export Citation Format

Share Document