Molecular cloning and expression of PoIR2, a novel gene involved in immune response in Japanese flounder (Paralichthys olivaceus)

2010 ◽  
Vol 9 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Shuo Li ◽  
Chunmei Li ◽  
Xubo Wang ◽  
Yanan Wang ◽  
Zhipeng Liu ◽  
...  
Keyword(s):  
Immune Response ◽  
Molecular Cloning ◽  
Paralichthys Olivaceus ◽  
Japanese Flounder ◽  
Cloning And Expression ◽  
Novel Gene

Molecular cloning and expression analysis of interferon gamma gene in Japanese flounder Paralichthys olivaceus

2008 ◽  
Vol 75 (1) ◽  
pp. 253-255 ◽  
Author(s):  
Tomomasa Matsuyama ◽  
Atushi Fujiwara ◽  
Takamitsu Sakai ◽  
Chihaya Nakayasu
Keyword(s):  
Molecular Cloning ◽  
Interferon Gamma ◽  
Expression Analysis ◽  
Paralichthys Olivaceus ◽  
Japanese Flounder ◽  
Cloning And Expression

scholarly journals Impact of Ringer’s Solution Challenge Stress to Immunostimulatory Experiment, Insights From Japanese Flounder

2020 ◽  
Vol 11 ◽  
Author(s):  
Jinxiang Liu ◽  
Zan Li ◽  
Yujue Wang ◽  
Quanqi Zhang
Keyword(s):  
Immune Response ◽  
Intraperitoneal Injection ◽  
Expression Profiles ◽  
Paralichthys Olivaceus ◽  
Metabolic Process ◽  
Japanese Flounder ◽  
Innate Immune ◽  
Fundamental Information ◽  
Immune Related Genes ◽  
The Impact

Ringer’s or phosphate buffer saline (PBS) solution buffer usually was used as dilution butter in intraperitoneal injection. Stress could activate immune response, inflammatory response and glycogen metabolic process. The impact of solution buffer as a stressor to immune system was ignored in immunostimulatory experiment. In this report, we tested the hypothesis that the innate immune response and glycogen metabolic process were altered when it were challenged with Ringer’s in Japanese flounder (Paralichthys olivaceus). RNA-seq was performed after challenge with Ringer’s at 8 h and 48 h. The data revealed that the expression profiles of blood, gill, and kidney were significantly changed. Differentially expressed genes (DEGs) were identified, and energy metabolic and immune-related genes were up-regulated or down-regulated obviously. GO and KEGG analyses showed that DEGs were mainly enriched in innate immune terms and pathways. Weighted gene co-expression networks analysis (WGCNA) also indicated the highest association module with stress. A total of 16 genes were detected in the gray module, which were immune-related and metabolic-related genes. These results provided fundamental information on intraperitoneal injection with solution buffer. It offered useful clues to further explore the functional mechanism of stress and immunity.

Molecular cloning and characterization of Toll-like receptor 14 in Japanese flounder, Paralichthys olivaceus

2011 ◽  
Vol 30 (1) ◽  
pp. 425-429 ◽  
Author(s):  
Seong Don Hwang ◽  
Hidehiro Kondo ◽  
Ikuo Hirono ◽  
Takashi Aoki
Keyword(s):  
Molecular Cloning ◽  
Paralichthys Olivaceus ◽  
Japanese Flounder ◽  
Toll Like Receptor
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