Spotted Sea Bass (Lateolabrax maculatus) cftr, nkcc1a, nkcc1b and nkcc2: Genome-Wide Identification, Characterization and Expression Analysis Under Salinity Stress

2019 ◽  
Vol 18 (6) ◽  
pp. 1470-1480 ◽  
Author(s):  
Kaiqiang Zhang ◽  
Xiaoyan Zhang ◽  
Haishen Wen ◽  
Xin Qi ◽  
Hongying Fan ◽  
...  
Keyword(s):  
Salinity Stress ◽  
Expression Analysis ◽  
Sea Bass ◽  
Genome Wide ◽  
Lateolabrax Maculatus

scholarly journals Evaluation of potential reference genes for quantitative RT-PCR analysis in spotted sea bass (Lateolabrax maculatus) under normal and salinity stress conditions

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5631 ◽  
Author(s):  
Haolong Wang ◽  
Haishen Wen ◽  
Yun Li ◽  
Kaiqiang Zhang ◽  
Yang Liu
Keyword(s):  
Salinity Stress ◽  
Reference Genes ◽  
18S Rrna ◽  
Sea Bass ◽  
Stress Conditions ◽  
Pcr Analysis ◽  
Stable Gene ◽  
Qrt Pcr ◽  
Lateolabrax Maculatus ◽  
Suitable Reference

The aim of this study was to select the most suitable reference genes for quantitative real-time polymerase chain reaction (qRT-PCR) of spotted sea bass (Lateolabrax maculatus), an important commercial marine fish in Pacific Asia, under normal physiological and salinity stress conditions. A total of 9 candidate reference genes (HPRT, GAPDH, EF1A, TUBA, RPL7, RNAPol II, B2M, ACTB and 18S rRNA) were analyzed by qRT-PCR in 10 tissues (intestine, muscle, stomach, brain, heart, liver, gill, kidney, pectoral fins and spleen) of L. maculatus. Four algorithms, geNorm, NormFinder, BestKeeper, and comparative ΔCt method, were used to evaluate the expression stability of the candidate reference genes. The results showed the 18S rRNA was most stable in different tissues under normal conditions. During salinity stress, RPL7 was the most stable gene according to overall ranking and the best combination of reference genes was RPL7 and RNAPol II. In contrast, GAPDH was the least stable gene which was not suitable as reference genes. The study showed that different algorithms might generate inconsistent results. Therefore, the combination of several reference genes should be selected to accurately calibrate system errors. The present study was the first to select reference genes of L. maculatus by qRT-PCR and provides a useful basis for selecting the appropriate reference gene in L. maculatus. The present study also has important implications for gene expression and functional genomics research in this species or other teleost species.

scholarly journals Genome-Wide SNP Discovery, Genotyping and Their Preliminary Applications for Population Genetic Inference in Spotted Sea Bass (Lateolabrax maculatus)

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157809 ◽  
Author(s):  
Juan Wang ◽  
Dong-Xiu Xue ◽  
Bai-Dong Zhang ◽  
Yu-Long Li ◽  
Bing-Jian Liu ◽  
...  
Keyword(s):  
Population Genetic ◽  
Sea Bass ◽  
Snp Discovery ◽  
Genome Wide ◽  
Lateolabrax Maculatus ◽  
Population Genetic Inference ◽  
Genetic Inference

Identification, expression analysis, and functional characterization of motilin and its receptor in spotted sea bass (Lateolabrax maculatus)

2019 ◽  
Vol 277 ◽  
pp. 38-48 ◽  
Author(s):  
Yangyang Zhou ◽  
Xin Qi ◽  
Haishen Wen ◽  
Kaiqiang Zhang ◽  
Xiaoyan Zhang ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Functional Characterization ◽  
Sea Bass ◽  
Lateolabrax Maculatus
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