Effect of Agitation Rate on Ethanol Production from Sugar Maple Hemicellulosic Hydrolysate by Pichia stipitis

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% deligniﬁcation, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous sacchariﬁcation and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

Download Full-text

Ethanol production by co-fermentation of hexose and pentose from food wastes using Saccharomyces coreanus and Pichia stipitis

Korean Journal of Chemical Engineering ◽

10.1007/s11814-011-0282-3 ◽

2012 ◽

Vol 29 (8) ◽

pp. 1038-1043 ◽

Cited By ~ 19

Author(s):

Seung-Mi Jeong ◽

Yong-Jin Kim ◽

Dong-Hoon Lee

Keyword(s):

Ethanol Production ◽

Pichia Stipitis ◽

Food Wastes

Download Full-text

Enhancing ethanol production from cellulosic sugars using Scheffersomyces (Pichia) stipitis

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-016-1580-2 ◽

2016 ◽

Vol 39 (7) ◽

pp. 1023-1032 ◽

Cited By ~ 9

Author(s):

C. C. Okonkwo ◽

M. M. Azam ◽

T. C. Ezeji ◽

N. Qureshi

Keyword(s):

Ethanol Production ◽

Pichia Stipitis ◽

Cellulosic Sugars

Download Full-text

Ethanol Production from Nondetoxified Dilute-Acid Lignocellulosic Hydrolysate by Cocultures of Saccharomyces cerevisiae Y5 and Pichia stipitis CBS6054

Biotechnology Research International ◽

10.1155/2012/656371 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 13

Author(s):

Ping Wan ◽

Dongmei Zhai ◽

Zhen Wang ◽

Xiushan Yang ◽

Shen Tian

Keyword(s):

Saccharomyces Cerevisiae ◽

Ethanol Production ◽

Ethanol Concentration ◽

Ethanol Yield ◽

Synthetic Medium ◽

Pichia Stipitis ◽

Dilute Acid ◽

Acid Hydrolysate ◽

Issatchenkia Orientalis ◽

Final Ethanol Concentration

Saccharomyces cerevisiae Y5 (CGMCC no. 2660) and Issatchenkia orientalis Y4 (CGMCC no. 2159) were combined individually with Pichia stipitis CBS6054 to establish the cocultures of Y5 + CBS6054 and Y4 + CBS6054. The coculture Y5 + CBS6054 effectively metabolized furfural and HMF and converted xylose and glucose mixture to ethanol with ethanol concentration of 16.6 g/L and ethanol yield of 0.46 g ethanol/g sugar, corresponding to 91.2% of the maximal theoretical value in synthetic medium. Accordingly, the nondetoxified dilute-acid hydrolysate was used to produce ethanol by co-culture Y5 + CBS6054. The co-culture consumed glucose along with furfural and HMF completely in 12 h, and all xylose within 96 h, resulting in a final ethanol concentration of 27.4 g/L and ethanol yield of 0.43 g ethanol/g sugar, corresponding to 85.1% of the maximal theoretical value. The results indicated that the co-culture of Y5 + CBS6054 was a satisfying combination for ethanol production from non-detoxified dilute-acid lignocellulosic hydrolysates. This co-culture showed a promising prospect for industrial application.

Download Full-text