Effects of Phytohormones and Jasmonic Acid on Glucosinolate Content in Hairy Root Cultures of Sinapis alba and Brassica rapa

2012 ◽  
Vol 169 (2) ◽  
pp. 624-635 ◽  
Author(s):  
Anja Kastell ◽  
Iryna Smetanska ◽  
Christian Ulrichs ◽  
Zhenzhen Cai ◽  
Inga Mewis
Keyword(s):  
Jasmonic Acid ◽  
Brassica Rapa ◽  
Hairy Root ◽  
Sinapis Alba ◽  
Glucosinolate Content ◽  
Hairy Root Cultures ◽  
Root Cultures

scholarly journals Enhanced production of valtrate in hairy root cultures of Valeriana jatamansi Jones by methyl jasmonate, jasmonic acid and salicylic acid elicitors

2020 ◽  
Vol 48 (2) ◽  
pp. 839-848
Author(s):  
Shuang ZHAO ◽  
Hong TANG
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Methyl Jasmonate ◽  
Hairy Root ◽  
Hairy Roots ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Potent Inducer ◽  
Valeriana Jatamansi ◽  
Pharmacologically Active

Valtrate is a pharmacologically active epoxyiridoid ester found in the roots and rhizomes of Valeriana jatamansi Jones. The plant produces only small amounts of this metabolite naturally, and so induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, strain R1601 of Agrobacterium rhizogenes was used to induce hairy roots in V. jatamansi, and stable hairy root cultures of V. jatamansi were established successfully. The influence of three exogenous elicitors including methyl jasmonate (MJ), jasmonic acid (JA) and salicylic acid (SA) on valtrate production in the hairy root cultures of V. jatamansi was also investigated, and the 25-day-old hairy root cultures were treated with different concentrations of the elicitors at exposure time of 7 days. This present study showed that MJ (100 mg/L) highly promoted valtrate production at 7 days after elicitation, to a level of 3.63 times higher than that of non-elicited control. SA did not significantly increase the production of valtrate. This is the first-time study to assess the elicitation of hairy root cultures to promote valtrate biosynthesis in V. jatamansi and the resulting experiments demonstrated that MJ was indeed a potent inducer of valtrate biosynthesis.

scholarly journals Glucosinolate Biosynthesis in Hairy Root Cultures of Broccoli (Brassica oleracea var. italica)

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800
Author(s):  
Sun-Ju Kim ◽  
Woo Tae Park ◽  
Md. Romij Uddin ◽  
Yeon Bok Kim ◽  
Sang-Yong Nam ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Hairy Root ◽  
Glucosinolate Content ◽  
Growth Media ◽  
Hairy Root Cultures ◽  
Ms Medium ◽  
Root Cultures ◽  
Valuable Alternative ◽  
Alternative Approach ◽  
Very High

Here we present previously unreported glucosinolate production by hairy root cultures of broccoli (B. oleracea var. italica). Growth media greatly influenced the growth and glucosinolate content of hairy root cultures of broccoli. Seven glucosinolates, glucoraphanin, gluconapin, glucoerucin, glucobrassicin, 4- methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin, were identified by analysis of the broccoli hairy root cultures. Both half and full strength B5 and SH media enabled the highest accumulation of glucosinolates. In most cases, the levels of glucosinolates were higher in SH and BS media. Among the 7 glucosinolates, the accumulation of neoglucobrassicin was very high, irrespective of growth medium. The neoglucobrassicin content was 7.4-fold higher in SH medium than 1/2 MS, in which its level was the lowest. The 1/2 B5 medium supported the production of the highest amounts of glucobrassicin and 4- methoxyglucobrassicin, the levels for which were 36.2- and 7.9- fold higher, respectively, than their lowest content in 1/2 MS medium. The 1/2 SH medium enabled the highest accumulation of glucoraphanin and gluconapin in the broccoli hairy root cultures, whose levels were 1.8- and 4.6-fold higher, respectively, than their lowest content in 1/2 MS medium. Our results suggest that hairy root cultures of broccoli could be a valuable alternative approach for the production of glucosinolate compounds.

scholarly journals The influence of yeast extract and jasmonic acid on phenolic acids content of in vitro hairy root cultures of Orthosiphon aristatus

10.5219/1508 ◽  
2021 ◽  
Vol 15 ◽  
pp. 1-8
Author(s):  
Iryna Smetanska ◽  
Oksana Tonkha ◽  
Tetjana Patyka ◽  
Dase Hunaefi ◽  
Diaa Mamdouh ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Phenolic Acids ◽  
Hairy Root ◽  
Yeast Extract ◽  
Acid Content ◽  
Control Plot ◽  
In Vitro Cultures ◽  
Hairy Root Cultures ◽  
Root Cultures

Phenolic acids represent a big group of plant secondary metabolites that can be used as food additives, nutraceuticals, and pharmaceuticals. Obtaining phenolic acids from the plant in vitro cultures provide an attractive alternative to produce high-value plant-derived products. The impact of yeast extract and jasmonic acid on the induction of defense responses and consequently the production of phenolic acids in vitro hairy root cultures of  O. aristatus have been investigated.  Treatment of O. aristatus cultures with jasmonic acid caused accumulation of 12.98 mg.g-1 DW of phenolic acids, elicitation with yeast extract resulted in the highest amount of phenolic acids, particularly in 17.99 mg.g-1 DW as compared to 4.03 mg.g-1 DW for the non-treated cultures. Individual phenolic acids showed a different response to elicitation. Particularly rosmarinic acid content on the control plot reached 2.89 mg.g-1 DW, while after the treatment with jasmonic acid is increased to 10.84 mg.g-1 DW and after yeast application, it was 14.31 mg.g-1 DW. Also, caffeic acid content increased until 0.75 and 2.01 mg.g-1 DW after application of jasmonic acid and yeast extract, while at the control plot its concentration was 0.58 mg.g-1 DW. Application of yeast extract influenced synthesis of phenolic acids in vitro cultures of O. aristatus stronger as jasmonic acid treatment.

Effect of jasmonic acid and chitosan on triterpenoid production in Calendula officinalis hairy root cultures

2019 ◽  
Vol 31 ◽  
pp. 5-11 ◽  
Author(s):  
Abdulwadood Shakir Mahmood Alsoufi ◽  
Cezary Pączkowski ◽  
Anna Szakiel ◽  
Marek Długosz
Keyword(s):  
Jasmonic Acid ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Calendula Officinalis

Influence of Auxins and Wounding on Glucosinolate Biosynthesis in Hairy Root Cultures of Chinese Cabbage (Brassica rapa ssp. pekinensis)

2015 ◽  
Vol 12 (2) ◽  
pp. 1041-1046
Author(s):  
Sun Ju Bong ◽  
Md. Romij Uddin ◽  
Sun-Ju Kim ◽  
Jong Seok Park ◽  
Sang Un Park
Keyword(s):  
Brassica Rapa ◽  
Hairy Root ◽  
Chinese Cabbage ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Glucosinolate Biosynthesis

Elicitor-enhanced Silymarin production in hairy root cultures of Silybum marianum L. Gaertn

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
T Hasanloo ◽  
H Rahnama ◽  
R Sepehrifar ◽  
MR Shams
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Silybum Marianum

Clone selection of in vitro horseradish hairy root cultures

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
R Bertóti ◽  
Á Alberti ◽  
A Böszörményi ◽  
R Könye ◽  
T Horváth ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Clone Selection ◽  
Selection Of
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