The influence of yeast extract and jasmonic acid on phenolic acids content of in vitro hairy root cultures of Orthosiphon aristatus

Phenolic acids represent a big group of plant secondary metabolites that can be used as food additives, nutraceuticals, and pharmaceuticals. Obtaining phenolic acids from the plant in vitro cultures provide an attractive alternative to produce high-value plant-derived products. The impact of yeast extract and jasmonic acid on the induction of defense responses and consequently the production of phenolic acids in vitro hairy root cultures of O. aristatus have been investigated. Treatment of O. aristatus cultures with jasmonic acid caused accumulation of 12.98 mg.g-1 DW of phenolic acids, elicitation with yeast extract resulted in the highest amount of phenolic acids, particularly in 17.99 mg.g-1 DW as compared to 4.03 mg.g-1 DW for the non-treated cultures. Individual phenolic acids showed a different response to elicitation. Particularly rosmarinic acid content on the control plot reached 2.89 mg.g-1 DW, while after the treatment with jasmonic acid is increased to 10.84 mg.g-1 DW and after yeast application, it was 14.31 mg.g-1 DW. Also, caffeic acid content increased until 0.75 and 2.01 mg.g-1 DW after application of jasmonic acid and yeast extract, while at the control plot its concentration was 0.58 mg.g-1 DW. Application of yeast extract influenced synthesis of phenolic acids in vitro cultures of O. aristatus stronger as jasmonic acid treatment.

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Clone selection of in vitro horseradish hairy root cultures

Planta Medica ◽

10.1055/s-0035-1565606 ◽

2015 ◽

Vol 81 (16) ◽

Author(s):

R Bertóti ◽

Á Alberti ◽

A Böszörményi ◽

R Könye ◽

T Horváth ◽

...

Keyword(s):

Hairy Root ◽

Hairy Root Cultures ◽

Root Cultures ◽

Clone Selection ◽

Selection Of

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In vitro Antioxidant, Anti-inflammatory, Anti-metabolic Syndrome, Antimicrobial, and Anticancer Effect of Phenolic Acids Isolated from Fresh Lovage Leaves [Levisticum officinale Koch] Elicited with Jasmonic Acid and Yeast Extract

Antioxidants ◽

10.3390/antiox9060554 ◽

2020 ◽

Vol 9 (6) ◽

pp. 554

Author(s):

Anna Jakubczyk ◽

Urszula Złotek ◽

Urszula Szymanowska ◽

Kamila Rybczyńska-Tkaczyk ◽

Krystyna Jęderka ◽

...

Keyword(s):

Metabolic Syndrome ◽

Jasmonic Acid ◽

Phenolic Acids ◽

Yeast Extract ◽

In Vitro Digestion ◽

Digestion Process ◽

Simulated Digestion ◽

Bioavailable Fraction ◽

Anti Inflammatory

Lovage seedlings were elicited with jasmonic acid (JA) and yeast extract (YE) to induce the synthesis of biologically active compounds. A simulated digestion process was carried out to determine the potential bioavailability of phenolic acids. Buffer extracts were prepared for comparison. The ability to neutralize ABTS radicals was higher in all samples after the in vitro digestion, compared to that in the buffer extracts. However, the elicitation resulted in a significant increase only in the value of the reduction power of the potentially bioavailable fraction of phenolic acids. The effect of the elicitation on the activity of the potentially bioavailable fraction of phenolic acids towards the enzymes involved in the pathogenesis of the metabolic syndrome, i.e., ACE, lipase, amylase, and glucosidase, was analyzed as well. The in vitro digestion caused a significant increase in the ability to inhibit the activity of these enzymes; moreover, the inhibitory activity against alpha-amylase was revealed only after the digestion process. The potential anti-inflammatory effect of the analyzed extracts was defined as the ability to inhibit key pro-inflammatory enzymes, i.e., lipoxygenase and cyclooxygenase 2. The buffer extracts from the YE-elicited lovage inhibited the LOX and COX-2 activity more effectively than the extracts from the control plants. A significant increase in the anti-inflammatory and antimicrobial properties was noted after the simulated digestion.

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Enhanced production of valtrate in hairy root cultures of Valeriana jatamansi Jones by methyl jasmonate, jasmonic acid and salicylic acid elicitors

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha48211891 ◽

2020 ◽

Vol 48 (2) ◽

pp. 839-848

Author(s):

Shuang ZHAO ◽

Hong TANG

Keyword(s):

Salicylic Acid ◽

Jasmonic Acid ◽

Methyl Jasmonate ◽

Hairy Root ◽

Hairy Roots ◽

Hairy Root Cultures ◽

Root Cultures ◽

Potent Inducer ◽

Valeriana Jatamansi ◽

Pharmacologically Active

Valtrate is a pharmacologically active epoxyiridoid ester found in the roots and rhizomes of Valeriana jatamansi Jones. The plant produces only small amounts of this metabolite naturally, and so induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, strain R1601 of Agrobacterium rhizogenes was used to induce hairy roots in V. jatamansi, and stable hairy root cultures of V. jatamansi were established successfully. The influence of three exogenous elicitors including methyl jasmonate (MJ), jasmonic acid (JA) and salicylic acid (SA) on valtrate production in the hairy root cultures of V. jatamansi was also investigated, and the 25-day-old hairy root cultures were treated with different concentrations of the elicitors at exposure time of 7 days. This present study showed that MJ (100 mg/L) highly promoted valtrate production at 7 days after elicitation, to a level of 3.63 times higher than that of non-elicited control. SA did not significantly increase the production of valtrate. This is the first-time study to assess the elicitation of hairy root cultures to promote valtrate biosynthesis in V. jatamansi and the resulting experiments demonstrated that MJ was indeed a potent inducer of valtrate biosynthesis.

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Establishment of Salvia castanea Diels f. tomentosa Stib. hairy root cultures and the promotion of tanshinone accumulation and gene expression with Ag+, methyl jasmonate, and yeast extract elicitation

PROTOPLASMA ◽

10.1007/s00709-015-0790-9 ◽

2015 ◽

Vol 253 (1) ◽

pp. 87-100 ◽

Cited By ~ 33

Author(s):

Bo Li ◽

Bangqing Wang ◽

Hongyan Li ◽

Liang Peng ◽

Mei Ru ◽

...

Keyword(s):

Gene Expression ◽

Methyl Jasmonate ◽

Hairy Root ◽

Yeast Extract ◽

Hairy Root Cultures ◽

Root Cultures

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Effects of Phytohormones and Jasmonic Acid on Glucosinolate Content in Hairy Root Cultures of Sinapis alba and Brassica rapa

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-012-0017-x ◽

2012 ◽

Vol 169 (2) ◽

pp. 624-635 ◽

Cited By ~ 30

Author(s):

Anja Kastell ◽

Iryna Smetanska ◽

Christian Ulrichs ◽

Zhenzhen Cai ◽

Inga Mewis

Keyword(s):

Jasmonic Acid ◽

Brassica Rapa ◽

Hairy Root ◽

Sinapis Alba ◽

Glucosinolate Content ◽

Hairy Root Cultures ◽

Root Cultures

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Hepatoprotective Triterpenes from Hairy Root Cultures of Ocimum basilicum L.

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-3-409 ◽

2009 ◽

Vol 64 (3-4) ◽

pp. 201-209 ◽

Cited By ~ 20

Author(s):

Amani M. Marzouk

Keyword(s):

Oxidative Stress ◽

Hairy Root ◽

Liver Homogenate ◽

Hepatoprotective Activity ◽

Ocimum Basilicum ◽

Albino Rats ◽

Hairy Root Cultures ◽

Root Cultures ◽

Oxidative Stress Status

Six triterpene acids identified as betulinic, oleanolic, ursolic, 3-epimaslinic, alphitolic and euscaphic acids have been isolated from a dichloromethane extract of hairy root cultures of Ocimum basilicum L. (Lamiaceae). These cultures were obtained by genetic transformation using Agrobacterium rhizogenes. The extract as well as the isolated compounds were evaluated for their hepatoprotective activity by measuring their effect on the oxidative stress status of liver, induced by carbon tetrachloride, in albino rats and in liver homogenate in vitro. All tested compounds displayed hepatoprotective activity comparable to oleanolic and ursolic acids.

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