Effective Stimulation of the Biotechnological Potential of the Medicinal White Rot Fungus: Phellinus pini by Menadione-Mediated Oxidative Stress

ABSTRACTLaccase is a copper-containing polyphenol oxidase that has great potential in industrial and biotechnological applications. Previous research has suggested that fungal laccase may be involved in the defense against oxidative stress, but there is little direct evidence supporting this hypothesis, and the mechanism by which laccase protects cells from oxidative stress also remains unclear. Here, we report that the expression of the laccase gene from white rot fungus inPichia pastoriscan significantly enhance the resistance of yeast to H2O2-mediated oxidative stress. The expression of laccase in yeast was found to confer a strong ability to scavenge intracellular H2O2and to protect cells from lipid oxidative damage. The mechanism by which laccase gene expression increases resistance to oxidative stress was then investigated further. We found that laccase gene expression inPichia pastoriscould increase the level of glutathione-based antioxidative activity, including the intracellular glutathione levels and the enzymatic activity of glutathione peroxidase, glutathione reductase, and γ-glutamylcysteine synthetase. The transcription of the laccase gene inPichia pastoriswas found to be enhanced by the oxidative stress caused by exogenous H2O2. The stimulation of laccase gene expression in response to exogenous H2O2stress further contributed to the transcriptional induction of the genes involved in the glutathione-dependent antioxidative system, includingPpYAP1,PpGPX1,PpPMP20,PpGLR1, andPpGSH1. Taken together, these results suggest that the expression of the laccase gene inPichia pastoriscan enhance the resistance of yeast to H2O2-mediated oxidative stress by stimulating the glutathione-based antioxidative system to protect the cell from oxidative damage.

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Effective and complex stimulation of the biodegradation system of fungus Cerrena unicolor by rapeseed meal fermentation

Acta Biochimica Polonica ◽

10.18388/abp.2015_1227 ◽

2016 ◽

Vol 63 (3) ◽

Cited By ~ 2

Author(s):

Magdalena Jaszek ◽

Justyna Miłek ◽

Jerzy Żuchowski ◽

Dawid Stefaniuk ◽

Monika Prendecka

Keyword(s):

Phenolic Compounds ◽

Rapeseed Meal ◽

White Rot ◽

White Rot Fungus ◽

Submerged Cultures ◽

Biotechnological Production ◽

Cerrena Unicolor ◽

Zymographic Analysis ◽

Stimulation Of ◽

Antioxidative Ability

The effect of supplementation of medium with rapeseed meal (RM) on production of biotechnologically important enzymes was investigated in submerged cultures of the white rot fungus Cerrena unicolor. The addition of RM (3.5% w/v) distinctly stimulated the activities of laccase, chitinase, and β-glucosidase. As compared to the control, the activities of chitinase, β-glucosidase, and laccase in the RM supplemented cultures were up to 4.1, 8.4, and 3.9 times higher, respectively. The results of the spectrophotometric and spectrofluorometric measurements were additionally confirmed by zymographic analysis of the samples. The level of sugars and phenolic compounds as well as the antioxidative ability of fungal preparations were also determined. The results obtained indicate that the submerged liquid fermentation of rapeseed meal can be proposed as an inexpensive and very effective method for biotechnological production of chitinase, β-glucosidase, and laccase by C. unicolor.

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Integrative visual omics of the white-rot fungus Polyporus brumalis exposes the biotechnological potential of its oxidative enzymes for delignifying raw plant biomass

Biotechnology for Biofuels ◽

10.1186/s13068-018-1198-5 ◽

2018 ◽

Vol 11 (1) ◽

Cited By ~ 7

Author(s):

Shingo Miyauchi ◽

Anaïs Rancon ◽

Elodie Drula ◽

Hayat Hage ◽

Delphine Chaduli ◽

...

Keyword(s):

Plant Biomass ◽

White Rot ◽

Oxidative Enzymes ◽

White Rot Fungus ◽

Biotechnological Potential ◽

Polyporus Brumalis

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INFLUENCES OF ETHYLENE STIMULATION OF RUBBER TREES (Hevea brasilliensis) ON THE EXTRACTIVES AND FUNGAL RESISTANCE OF LUMBER

CERNE ◽

10.1590/01047760201622032183 ◽

2016 ◽

Vol 22 (3) ◽

pp. 223-232 ◽

Cited By ~ 1

Author(s):

Banyat Cherdchim ◽

Jareeya Satansat

Keyword(s):

Ganoderma Lucidum ◽

Brown Rot ◽

Fungal Resistance ◽

White Rot ◽

White Rot Fungus ◽

Rubber Latex ◽

Brown Rot Fungus ◽

Wood Extracts ◽

Stimulation Of

ABSTRACT Ethylene stimulation increases the rubber latex yield of live rubberwood (Hevea brasiliensis). Lumber samples from ethylene treated rubberwood (TRW) and from untreated rubberwood (URW) were compared mainly for their resistance to fungi, differences in the chemical composition between TRW and URW, and the antifungal activities of their aqueous extracts. The TRW had significantly higher lignin and extractives contents than the URW, but the TRW had comparatively poor resistance to fungal rotting. The white rot fungus Ganoderma lucidum and the brown rot fungus Gloeophyllum striantum caused in vitro significantly higher mass loss in TRW than in URW. This might be related to the phenolic compounds 2,4-ditert-butylphenol and 4-hydroxy-3,5- dimethoxy-benzaldehyde. The aqueous wood extracts strongly inhibited growth of G. lucidum, with lesser effects on the other fungi tested. Caffeine was detected in the TRW, but not the URW. However, the caffeine degraded so quickly that it had no effect on the 6 and 12 weeks fungal resistances of wood samples.

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Decolorization and biodegradation of melanoidin contained in beet molasses by an anamorphic strain of Bjerkandera adusta CCBAS930 and its mutants

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-020-02944-w ◽

2020 ◽

Vol 37 (1) ◽

Author(s):

Teresa Korniłłowicz-Kowalska ◽

Kamila Rybczyńska-Tkaczyk

Keyword(s):

Free Radicals ◽

Phenolic Compounds ◽

Peroxidase Activity ◽

Aerial Mycelium ◽

Culture Method ◽

White Rot ◽

White Rot Fungus ◽

Bjerkandera Adusta ◽

Beet Molasses ◽

Stimulation Of

AbstractWe used a ligninolytic strain of the white-rot fungus B. adusta CCBAS 930 and its mutants with modified ligninolytic activity to assess their potential to remove of molasses. The analyzed strains have been shown to be able to decolorize 1% or 2% molasses solutions containing brown-colored toxic melanoidins. It was found that the decolorization process was determined by the transition to the stage of production of sporulating aerial mycelium (liquid and agar cultures) coupled with an increase in peroxidase activity, which was accompanied by a decrease in the level of melanoidin, free radicals, and phenolic compounds. Four different peroxidase activities were detected in post-culture liquids, i.e. horseradish-like (HRP-like), manganese-dependent (MnP), lignin (LiP), and versatile (VP) peroxidase activities. The HRP-like peroxidase was characterized by the highest activity. The efficiency of removal of melanoidins from a 1% molasses solution by the parental strain and the mutants was dependent on the culture method. The highest efficiency was noted in immobilized cultures (threefold higher than in the mycelium-free cultures), which was accompanied by stimulation of HRP-like peroxidase activity. Mutant 930-5 was found to be the most effective in the decolorization and decomposition of melanoidin. The HRP-like activity in the immobilized cultures of B. adusta 930-5 was 640-fold higher than in the mycelium-free cultures of the fungus. Moreover, decolorization and biodegradation of melanoidin by B. adusta CCBAS 930 and 930-5 was coupled with detoxification. Graphic abstract

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