Human Embryonic Stem Cells (hESCs) Cultured Under Distinctive Feeder-Free Culture Conditions Display Global Gene Expression Patterns Similar to hESCs from Feeder-Dependent Culture Conditions

2010 ◽  
Vol 6 (3) ◽  
pp. 425-437 ◽  
Author(s):  
Tae-Min Yoon ◽  
Bomi Chang ◽  
Hyeung-Taek Kim ◽  
Joo-Hyun Jee ◽  
Dong-Wook Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Expression Patterns ◽  
Embryonic Stem ◽  
Global Gene Expression ◽  
Culture Conditions ◽  
Gene Expression Patterns

scholarly journals MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells

2009 ◽  
Vol 7 (1) ◽  
pp. 20 ◽  
Author(s):  
Jiaqiang Ren ◽  
Ping Jin ◽  
Ena Wang ◽  
Francesco M Marincola ◽  
David F Stroncek
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Expression Patterns ◽  
Embryonic Stem ◽  
Gene Expression Patterns

scholarly journals Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors

2003 ◽  
Vol 100 (23) ◽  
pp. 13350-13355 ◽  
Author(s):  
J. M. Sperger ◽  
X. Chen ◽  
J. S. Draper ◽  
J. E. Antosiewicz ◽  
C. H. Chon ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Human Embryonic Stem Cells ◽  
Expression Patterns ◽  
Germ Cell Tumors ◽  
Embryonic Stem ◽  
Gene Expression Patterns

scholarly journals Impact of AHR Ligand TCDD on Human Embryonic Stem Cells and Early Differentiation

2020 ◽  
Vol 21 (23) ◽  
pp. 9052
Author(s):  
Indrek Teino ◽  
Antti Matvere ◽  
Martin Pook ◽  
Inge Varik ◽  
Laura Pajusaar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Target Genes ◽  
Expression Patterns ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Cell Types ◽  
Early Differentiation

Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor, which mediates the effects of a variety of environmental stimuli in multiple tissues. Recent advances in AHR biology have underlined its importance in cells with high developmental potency, including pluripotent stem cells. Nonetheless, there is little data on AHR expression and its role during the initial stages of stem cell differentiation. The purpose of this study was to investigate the temporal pattern of AHR expression during directed differentiation of human embryonic stem cells (hESC) into neural progenitor, early mesoderm and definitive endoderm cells. Additionally, we investigated the effect of the AHR agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the gene expression profile in hESCs and differentiated cells by RNA-seq, accompanied by identification of AHR binding sites by ChIP-seq and epigenetic landscape analysis by ATAC-seq. We showed that AHR is differentially regulated in distinct lineages. We provided evidence that TCDD alters gene expression patterns in hESCs and during early differentiation. Additionally, we identified novel potential AHR target genes, which expand our understanding on the role of this protein in different cell types.

Metabolic flux analysis of embryonic stem cells using three distinct differentiation protocols and comparison to gene expression patterns

2010 ◽  
Vol 26 (5) ◽  
pp. 1222-1229 ◽  
Author(s):  
Darío E. Sepúlveda ◽  
Barbara A. Andrews ◽  
Eleftherios Terry Papoutsakis ◽  
Juan A. Asenjo
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Expression Patterns ◽  
Embryonic Stem ◽  
Gene Expression Patterns ◽  
Flux Analysis

scholarly journals Mitochondrial DNA Haplotypes Define Gene Expression Patterns in Pluripotent and Differentiating Embryonic Stem Cells

Stem Cells ◽  
2013 ◽  
Vol 31 (4) ◽  
pp. 703-716 ◽  
Author(s):  
Richard D.W. Kelly ◽  
Andrew E. Rodda ◽  
Adam Dickinson ◽  
Arsalan Mahmud ◽  
Christian M. Nefzger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mitochondrial Dna ◽  
Embryonic Stem Cells ◽  
Expression Patterns ◽  
Embryonic Stem ◽  
Gene Expression Patterns

CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic gene expression pattern

Blood ◽  
2004 ◽  
Vol 103 (11) ◽  
pp. 4134-4141 ◽  
Author(s):  
Shi-Jiang Lu ◽  
Fei Li ◽  
Loyda Vida ◽  
George R. Honig
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Fetal Liver ◽  
Expression Patterns ◽  
Embryonic Stem ◽  
Gene Expression Pattern ◽  
Early Developmental Stage ◽  
In Cells

Abstract Gene expression patterns of CD34+CD38- cells derived from human embryonic stem cells (ESCs) were compared with those of cells isolated from adult human bone marrow (BM) using microarrays; 1692 and 1494 genes were expressed at levels at least 3-fold above background in cells from BM and ESCs, respectively. Of these, 494 showed similar levels of expression in cells from both sources, 791 genes were overexpressed in cells from BM (BM versus ESCs, at least 2-fold), and 803 genes were preferentially expressed in cells from ESCs (ESCs versus BM, at least 2-fold). The message of the flt-3 gene was markedly decreased in cells from ESCs, whereas there was substantial flt-3 expression in cells from BM. High levels of embryonic ϵ-globin expression were observed—but no adult β-globin message—in CD34+CD38- cells from ESCs, whereas high levels of β-globin expression—but no embryonic ϵ-globin message—could be detected in cells from BM. Furthermore, high levels of major histocompatibility complex (MHC) gene expression were demonstrated in cells from BM but very low levels of MHC message in corresponding cells from ESCs. These observations demonstrate that CD34+CD38- cells derived from ESCs correspond consistently to an early developmental stage at which the yolk sac and fetal liver are the primary sites of hematopoiesis.

Microarray analysis of gene expression patterns during neuronal differentiation of embryonic stem cells

10.1038/14356 ◽  
1999 ◽  
Vol 23 (S3) ◽  
pp. 59-60
Author(s):  
J.F. Loring ◽  
R. Wesselschmidt ◽  
S. Fuhrman ◽  
X. Wen ◽  
R. Somogyi
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neuronal Differentiation ◽  
Microarray Analysis ◽  
Expression Patterns ◽  
Embryonic Stem ◽  
Gene Expression Patterns

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression

scholarly journals The Trends in Global Gene Expression in Mouse Embryonic Stem Cells During Spaceflight

2019 ◽  
Vol 10 ◽  
Author(s):  
Lili An ◽  
Yanming Li ◽  
Yingjun Fan ◽  
Ning He ◽  
Fanlei Ran ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Global Gene Expression ◽  
Mouse Embryonic Stem Cells
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