Directed Differentiation of Embryonic Stem Cells Allows Exploration of Novel Transcription Factor Genes for Pancreas Development

2012 ◽  
Vol 8 (3) ◽  
pp. 803-812 ◽  
Author(s):  
Jing Sui ◽  
Munish Mehta ◽  
Bingyin Shi ◽  
Grant Morahan ◽  
Fang-Xu Jiang
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Directed Differentiation ◽  
Pancreas Development ◽  
Transcription Factor Genes

scholarly journals Transcription factor directed differentiation of human embryonic stem cells into the pancreatic endocrine lineage

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S109-S109 ◽  
Author(s):  
Qing Le Liang ◽  
Xiao Xun Wang ◽  
Xiao Fei Yan ◽  
Li Jun Yang ◽  
Dong Qi Tang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Directed Differentiation

scholarly journals Transcription factor directed differentiation of embryonic stem cells in vitro

2009 ◽  
Vol 92 (3) ◽  
pp. S172
Author(s):  
R.M. Anchan ◽  
S.A. Lachke ◽  
B. Gerami-Naini ◽  
K.E. Naber ◽  
J. Eaton ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Directed Differentiation

Positive regulators of the lineage-specific transcription factor GATA-1 in differentiating erythroid cells

1994 ◽  
Vol 14 (5) ◽  
pp. 3108-3114
Author(s):  
M H Baron ◽  
S M Farrington
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Transcription Factor ◽  
Embryonic Stem Cells ◽  
Cultured Cells ◽  
Globin Gene ◽  
Embryonic Stem ◽  
Targeted Mutagenesis ◽  
Erythroid Cell ◽  
Erythroid Cells

The zinc finger transcription factor GATA-1 is a major regulator of gene expression in erythroid, megakaryocyte, and mast cell lineages. GATA-1 binds to WGATAR consensus motifs in the regulatory regions of virtually all erythroid cell-specific genes. Analyses with cultured cells and cell-free systems have provided strong evidence that GATA-1 is involved in control of globin gene expression during erythroid differentiation. Targeted mutagenesis of the GATA-1 gene in embryonic stem cells has demonstrated its requirement in normal erythroid development. Efficient rescue of the defect requires an intact GATA element in the distal promoter, suggesting autoregulatory control of GATA-1 transcription. To examine whether GATA-1 expression involves additional regulatory factors or is maintained entirely by an autoregulatory loop, we have used a transient heterokaryon system to test the ability of erythroid factors to activate the GATA-1 gene in nonerythroid nuclei. We show here that proerythroblasts and mature erythroid cells contain a diffusible activity (TAG) capable of transcriptional activation of GATA-1 and that this activity decreases during the terminal differentiation of erythroid cells. Nuclei from GATA-1- mutant embryonic stem cells can still be reprogrammed to express their globin genes in erythroid heterokaryons, indicating that de novo induction of GATA-1 is not required for globin gene activation following cell fusion.

scholarly journals Directed differentiation of telencephalic precursors from embryonic stem cells

2005 ◽  
Vol 8 (3) ◽  
pp. 288-296 ◽  
Author(s):  
Kiichi Watanabe ◽  
Daisuke Kamiya ◽  
Ayaka Nishiyama ◽  
Tomoko Katayama ◽  
Satoshi Nozaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Directed Differentiation
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