Culture Variabilities of Human iPSC-Derived Cerebral Organoids Are a Major Issue for the Modelling of Phenotypes Observed in Alzheimer’s Disease

2021 ◽  
Author(s):  
Damián Hernández ◽  
Louise A. Rooney ◽  
Maciej Daniszewski ◽  
Lerna Gulluyan ◽  
Helena H. Liang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Human Ipsc

[P3-170]: HUMAN IPSC-DERIVED ALZHEIMER's DISEASE ASTROCYTES RECAPITULATE DISEASE-RELATED PHENOTYPES

2017 ◽  
Vol 13 (7S_Part_21) ◽  
pp. P999-P1000
Author(s):  
Minna Oksanen ◽  
Andrew J. Petersen ◽  
Katja Puttonen ◽  
Riikka H. Hämäläinen ◽  
Šárka Lehtonen ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Human Ipsc

scholarly journals Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer’s disease and three sex- and age-matched healthy controls

2021 ◽  
Vol 53 ◽  
pp. 102378
Author(s):  
Jan Raska ◽  
Hana Klimova ◽  
Katerina Sheardova ◽  
Veronika Fedorova ◽  
Hana Hribkova ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Late Onset ◽  
Healthy Controls ◽  
Human Ipsc

scholarly journals APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer’s disease patient iPSC-derived cerebral organoids

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jing Zhao ◽  
Yuan Fu ◽  
Yu Yamazaki ◽  
Yingxue Ren ◽  
Mary D. Davis ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Healthy Subject ◽  
Late Onset ◽  
Disease Patient ◽  
Underlying Mechanism ◽  
Synapse Loss ◽  
Induced Pluripotent ◽  
Human Ipsc ◽  
Normal Cognition

Abstract APOE4 is the strongest genetic risk factor associated with late-onset Alzheimer’s disease (AD). To address the underlying mechanism, we develop cerebral organoid models using induced pluripotent stem cells (iPSCs) with APOE ε3/ε3 or ε4/ε4 genotype from individuals with either normal cognition or AD dementia. Cerebral organoids from AD patients carrying APOE ε4/ε4 show greater apoptosis and decreased synaptic integrity. While AD patient-derived cerebral organoids have increased levels of Aβ and phosphorylated tau compared to healthy subject-derived cerebral organoids, APOE4 exacerbates tau pathology in both healthy subject-derived and AD patient-derived organoids. Transcriptomics analysis by RNA-sequencing reveals that cerebral organoids from AD patients are associated with an enhancement of stress granules and disrupted RNA metabolism. Importantly, isogenic conversion of APOE4 to APOE3 attenuates the APOE4-related phenotypes in cerebral organoids from AD patients. Together, our study using human iPSC-organoids recapitulates APOE4-related phenotypes and suggests APOE4-related degenerative pathways contributing to AD pathogenesis.

Human iPSC-derived neurons and astrocytes as models of neurodegeneration, neuroinflammation and vesicular trafficking in Alzheimer's disease

2018 ◽  
Vol 120 ◽  
pp. S158 ◽  
Author(s):  
Sara Pinto ◽  
Sofia Ferreira ◽  
Filipa Moreira ◽  
Ana Ribeiro ◽  
Carolina Cunha ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Vesicular Trafficking ◽  
Human Ipsc

scholarly journals APOE4 Causes Widespread Molecular and Cellular Alterations Associated with Alzheimer’s Disease Phenotypes in Human iPSC-Derived Brain Cell Types

Neuron ◽  
2018 ◽  
Vol 98 (6) ◽  
pp. 1141-1154.e7 ◽  
Author(s):  
Yuan-Ta Lin ◽  
Jinsoo Seo ◽  
Fan Gao ◽  
Heather M. Feldman ◽  
Hsin-Lan Wen ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cell Types ◽  
Brain Cell ◽  
Disease Phenotypes ◽  
Human Ipsc ◽  
Brain Cell Types ◽  
Cellular Alterations

scholarly journals Drug repurposing for Alzheimer’s disease based on transcriptional profiling of human iPSC-derived cortical neurons

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gareth Williams ◽  
Ariana Gatt ◽  
Earl Clarke ◽  
Jonathan Corcoran ◽  
Patrick Doherty ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cortical Neurons ◽  
Transcriptional Profiling ◽  
Drug Repurposing ◽  
Human Ipsc
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