Age-Dependent Differences in Recovered Visual Responses in Royal College of Surgeons Rats Transduced with the Channelrhodopsin-2 Gene

2011 ◽  
Vol 46 (2) ◽  
pp. 393-400 ◽  
Author(s):  
Hitomi Isago ◽  
Eriko Sugano ◽  
Zhuo Wang ◽  
Namie Murayama ◽  
Eri Koyanagi ◽  
...  
Keyword(s):  
Royal College ◽  
Visual Responses ◽  
Age Dependent

scholarly journals Development of an optogenetic gene sensitive to daylight and its implications in vision restoration

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Yoshito Watanabe ◽  
Eriko Sugano ◽  
Kitako Tabata ◽  
Akito Hatakeyama ◽  
Tetsuya Sakajiri ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Amino Acid ◽  
Royal College ◽  
Visual Function ◽  
Photoreceptor Cells ◽  
Light Sensitivity ◽  
Visual Responses ◽  
Extracellular Loops ◽  
Ion Conducting ◽  
Homologous Amino Acid

AbstractOptogenetic gene-mediated therapy for restoring vision is thought to be a useful treatment for blind patients. However, light sensitivity achieved using this gene therapy is inferior to that of daylight vision. To increase light sensitivity, we designed three mutants using a bioinformatics approach. Nucleotide sequences encoding two sites in the extracellular loops (ex1, ex3) of mVChR1 close to simulated ion-conducting pathways were replaced by homologous amino acid-encoding sequences of ChR1 or ChR2. The light sensitivity of ex3mV1 was higher than that of mVChR1 at 405–617 nm. Visual responses were restored in Royal College of Surgeons rats with genetically degenerating photoreceptor cells transfected with ex3mV1Co, wherein transmembrane of sixth (TM6) in ex3mV1 was additionally replaced with the corresponding domain of CoChR; these rats responded to light in the order of μW/mm2. Thus, ex3mV1Co might be useful for the restoration of advanced visual function.

Effects of Hyperoxia on Lung Utrastructure

1970 ◽  
Vol 28 ◽  
pp. 26-27
Author(s):  
Gladys Harrison
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Light Microscopy ◽  
Oxygen Effects ◽  
Age Dependent ◽  
The Central Nervous System ◽  
The Subject ◽  
Space Age ◽  
Alveolar Septa ◽  
Extensive Damage

With the advent of the space age and the need to determine the requirements for a space cabin atmosphere, oxygen effects came into increased importance, even though these effects have been the subject of continuous research for many years. In fact, Priestly initiated oxygen research when in 1775 he published his results of isolating oxygen and described the effects of breathing it on himself and two mice, the only creatures to have had the “privilege” of breathing this “pure air”.Early studies had demonstrated the central nervous system effects at pressures above one atmosphere. Light microscopy revealed extensive damage to the lungs at one atmosphere. These changes which included perivascular and peribronchial edema, focal hemorrhage, rupture of the alveolar septa, and widespread edema, resulted in death of the animal in less than one week. The severity of the symptoms differed between species and was age dependent, with young animals being more resistant.

1248: Impact of Surgical Treatment of Renal Carcinoma on Renal Function and Blood Pressure: A Race- and Age-Dependent Association

2007 ◽  
Vol 177 (4S) ◽  
pp. 411-412
Author(s):  
Javier Miller ◽  
Angela Smith ◽  
Kris Gunn ◽  
Erik Kouba ◽  
Eric M. Wallen ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Renal Function ◽  
Surgical Treatment ◽  
Renal Carcinoma ◽  
Age Dependent
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