Molecular Confirmation of Intraspecific Tomato (Solanum lycopersicum) Hybrids and Their Evaluation Against Late Blight and Cucumber Mosaic Virus

2017 ◽  
Vol 59 (6) ◽  
pp. 234-240 ◽  
Author(s):  
Amjad Hameed ◽  
Muhammad Yussouf Saleem ◽  
Khalid Pervaiz Akhtar ◽  
Muhammad Shoaib ◽  
Qumer Iqbal ◽  
...  
Keyword(s):  
Late Blight ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Solanum Lycopersicum

scholarly journals Nuevos registros de aislamientos del Cucumber mosaic virus y su RNA satélite en Colima, México

2019 ◽  
Vol 37 (2) ◽  
Author(s):  
Pedro Valadez-Ramírez ◽  
Javier Paz-Román ◽  
Salvador Guzmán-González ◽  
Marco Tulio Buenrostro-Nava ◽  
Daniel Leobardo Ochoa-Martínez
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Solanum Lycopersicum ◽  
Capsicum Annuum ◽  
Catharanthus Roseus ◽  
Cucumis Melo ◽  
Rt Pcr ◽  
Impacto Económico

El <em>Cucumber mosaic virus</em> (CMV) ocasiona una de las enfermedades virales más importantes a nivel mundial en plantas silvestres y cultivadas. En México son pocos los estudios que se han abordado con este virus, y dada su amplia gama de hospedantes e impacto económico, es necesario contar con mayor información de su presencia y distribución en zonas de importancia agrícola como las del estado de Colima. En este trabajo, se reportan nuevos aislamientos del CMV identificados por RT-PCR, secuenciación de DNA y su análisis filogenético: CMV-Vin en vinca (<em>Catharanthus roseus</em>), CMV-Chi en chile jalapeño (<em>Capsicum annuum</em>) y CMV-Tom en tomate saladette (<em>Solanum lycopersicum</em>). Se confirmó, además, la presencia del CMV en melón cantaloupe (<em>Cucumis melo</em>) (CMV-Mel). Los aislamientos CMV-Vin, CMV-Chi y CMV-Mel agruparon en el subgrupo IB, mientras que CMV-Tom agrupó en el subgrupo IA de CMV. De estos aislamientos, sólo CMV-Vin evidenció la presencia de un RNA satélite (satRNA Vin) sin dominio necrogénico. Este es el primer reporte de la presencia del CMV en vinca, chile y tomate y de un RNA satélite en vinca en Colima, México.

Phylogeny of Egyptian isolates of Cucumber mosaic virus (CMV) and Tomato mosaic virus (ToMV) infecting Solanum lycopersicum

2017 ◽  
Vol 149 (1) ◽  
pp. 219-225 ◽  
Author(s):  
Mona Rabie ◽  
Claudio Ratti ◽  
Matteo Calassanzio ◽  
Engy Abdel Aleem ◽  
Faiza Aref Fattouh
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Solanum Lycopersicum ◽  
Tomato Mosaic Virus

scholarly journals UJI KETAHANAN TIGA VARIETAS TOMAT (SOLANUM LYCOPERSICUM L.) TERHADAP SERANGAN CMV (CUCUMBER MOSAIC VIRUS) DENGAN METODE DAS-ELISA

2017 ◽  
Vol 1 (2) ◽  
pp. 101
Author(s):  
Lia Agturani Tudaryati ◽  
Febi Nurilmala ◽  
Krisna Dwiharniati
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Solanum Lycopersicum ◽  
Incubation Period ◽  
Positive Control ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tomato Plants ◽  
Solanum Lycopersicum L ◽  
Das Elisa

Endurance Test Three Tomato Variety (Solanum lycopersicum L.) Against Attacks CMV (Cucumber Mosaic Virus) DAS-ELISA Method          Tomato plantation intensification can be done by controlling nuisance organism plant causes disease in tomato, such as CMV. CMV can be transmitted mechanically, and therefore testing of tomato varieties resistance to CMV can be performed with CMV isolates infect mechanically to healthy tomato plants (variety Marta F1, San Marino, and Viccario). CMV isolates derived from two sources, namely a positive tomato plants attacked by CMV (plant sap) and the positive control CMV AGDIA producers commonly used as a positive control test DAS-ELISA (Double Antibody Sandwich-Enzyme Linked immunosorbent assay). This study aims to test three varieties of tomato resistance against CMV attacks. Research conducted in the screen house and laboratory virology Central Agricultural Quarantine Standards Test. Identification of CMV infection was done by observing the incubation period, symptoms appeared, and continued with DAS-ELISA test. Structured treatment completely randomized design (CRD) with 6 replications. Data processed with fingerprint analysis and continued with various multiple Duncan test at 5% level test. The results showed the disease symptoms appeared only on the varieties of San Marino from CMV infected plant sap. Symptoms caused a reduction of leaf rolling and leaf lamina 8-10 day incubation period and symptoms of stunted stems with 14-28 day incubation period, the San Marino CMV causes the reduction of plant height by 33%. Sensitivity of tomato varieties against CMV San Marino sap from plants was quantitatively evidenced by positive results on the DAS-ELISA test. Meanwhile, F1 and Marta varieties resistant to Viccario CMV infection sap from plants and the positive control CMV AGDIA producers. Keywords : Tomato (Solanum Lycopersicum l.), Cucumber Mosaic Virus, DAS-ELISA method ABSTRAK                 Intensifikasi perkebunan tomat dapat dilakukan dengan mengendalikan organisme pengganggu tanaman (OPT) penyebab penyakit pada tomat, seperti CMV. CMV dapat ditularkan secara mekanis, oleh karena itu pengujian ketahanan varietas tomat terhadap CMV dapat dilakukan dengan menularkan isolat CMV secara mekanik kepada tanaman tomat sehat (varietas Marta F1, San Marino, dan Viccario). Isolat CMV berasal dari dua sumber, yaitu tanaman tomat yang positif terserang CMV (sap tanaman) dan kontrol positif CMV produsen AGDIA yang biasa digunakan sebagai kontrol positif pengujian DAS-ELISA (Double Antibody Sandwich-Enzyme Linked Immunosorbent assay). Penelitian ini bertujuan untuk menguji ketahanan tiga varietas tomat terhadap serangan CMV. Penelitian dilaksanakan di screen house dan laboratorium virologi Balai Besar Uji Standar Karantina Pertanian pada bulan Januari sampai April 2008. Identifikasi hasil penularan CMV dilakukan dengan mengamati periode inkubasi, gejala yang muncul, dan dilanjutkan dengan pengujian DAS-ELISA. Perlakuan disusun dengan rancangan acak lengkap (RAL) dengan 6 ulangan. Data diolah dengan analisis sidik ragam dan dilanjutkan dengan uji berganda Duncan pada taraf uji 5%. Hasil penelitian menunjukkan gejala penyakit hanya muncul pada varietas San Marino yang ditulari CMV asal sap tanaman. Gejala yang ditimbulkan berupa daun menggulung dan reduksi lamina daun dengan masa inkubasi 8-10 hari, serta gejala batang kerdil dengan masa inkubasi 14-28 hari, CMV pada San Marino menyebabkan reduksi tinggi tanaman sebesar 33%. Kesensitifan tomat varietas San Marino terhadap CMV asal sap tanaman secara kuantitatif dibuktikan dengan hasil yang positif pada pengujian DAS-ELISA. Sedangkan, varietas Marta F1 dan Viccario tahan terhadap penularan CMV asal sap tanaman maupun kontrol positif CMV produsen AGDIA.Kata kunci : Tomat (Solanum lycopersicum  L.), Cucumber Mosaic Virus, metode DAS-ELISA

Screening of Capsicum Accessions for Resistance to Cucumber Mosaic Virus

1997 ◽  
Vol 65 (4) ◽  
pp. 769-776 ◽  
Author(s):  
Shinji Monma ◽  
Yoshiteru Sakata
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus

scholarly journals Six New Subgroup I Members of Japanese Cucumber Mosaic Virus as Determined by Nucleotide Sequence Analysis on RNA3's cDNAs.

1996 ◽  
Vol 62 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Piyasak CHAUMPLUK ◽  
Yukiko SASAKI ◽  
Naoko NAKAJIMA ◽  
Hideaki NAGANO ◽  
Ikuo NAKAMURA ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Nucleotide Sequence Analysis

scholarly journals Cucumber mosaic virus-induced gene silencing in banana

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yuh Tzean ◽  
Ming-Chi Lee ◽  
Hsiao-Hsuan Jan ◽  
Yi-Shu Chiu ◽  
Tsui-Chin Tu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Induced Gene Silencing

scholarly journals Topical Application of Double-Stranded RNA Targeting 2b and CP Genes of Cucumber mosaic virus Protects Plants against Local and Systemic Viral Infection

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 963
Author(s):  
Maria C. Holeva ◽  
Athanasios Sklavounos ◽  
Rajendran Rajeswaran ◽  
Mikhail M. Pooggin ◽  
Andreas E. Voloudakis
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Topical Application ◽  
Plant Virus ◽  
Plant Protection ◽  
Post Inoculation ◽  
Tobacco Plants ◽  
Double Stranded Rna

Cucumber mosaic virus (CMV) is a destructive plant virus with worldwide distribution and the broadest host range of any known plant virus, as well as a model plant virus for understanding plant–virus interactions. Since the discovery of RNA interference (RNAi) as a major antiviral defense, RNAi-based technologies have been developed for plant protection against viral diseases. In plants and animals, a key trigger of RNAi is double-stranded RNA (dsRNA) processed by Dicer and Dicer-like (DCL) family proteins in small interfering RNAs (siRNAs). In the present study, dsRNAs for coat protein (CP) and 2b genes of CMV were produced in vitro and in vivo and applied onto tobacco plants representing a systemic solanaceous host as well as on a local host plant Chenopodium quinoa. Both dsRNA treatments protected plants from local and systemic infection with CMV, but not against infection with unrelated viruses, confirming sequence specificity of antiviral RNAi. Antiviral RNAi was effective when dsRNAs were applied simultaneously with or four days prior to CMV inoculation, but not four days post inoculation. In vivo-produced dsRNAs were more effective than the in vitro-produced; in treatments with in vivo dsRNAs, dsRNA-CP was more effective than dsRNA-2b, while the effects were opposite with in vitro dsRNAs. Illumina sequencing of small RNAs from in vivo dsRNA-CP treated and non-treated tobacco plants revealed that interference with CMV infection in systemic leaves coincides with strongly reduced accumulation of virus-derived 21- and 22-nucleotide (nt) siRNAs, likely generated by tobacco DCL4 and DCL2, respectively. While the 21-nt class of viral siRNAs was predominant in non-treated plants, 21-nt and 22-nt classes accumulated at almost equal (but low) levels in dsRNA treated plants, suggesting that dsRNA treatment may boost DCL2 activity. Taken together, our findings confirm the efficacy of topical application of dsRNA for plant protection against viruses and shed more light on the mechanism of antiviral RNAi.

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