Genetic diversity in barley landraces (Hordeum vulgare L. subsp. vulgare) originated from Crescent Fertile region as detected by seed storage proteins

AbstractEvaluation of the genetic diversity present within species is essential for conservation, management and utilization of the genetic resources. The objective of this study was to evaluate genetic variability of 70 common bean genotypes for seed storage proteins, grain morphological characteristics and agronomic traits. Two methods of extracting soluble seed proteins in salt were used.Positive correlations were observed among both seed morphological characters and developmental characters while yield components showed negative correlations with each other. Factor analysis for agronomic and grain morphological traits resulted in three factors were named yield components, seed morphology and phenology, respectively. Most genotypes had lower or medium scores for yield components and phenology factors. Considerable diversity was observed for seed morphology factor among the common bean genotypes.Nei’s diversity coefficient (He= 0.4), effective number of alleles (Ae= 1.69) and number of polymorphic loci (N = 17) indicated larger variation in the extraction method of soluble proteins in low salt (0.2 M NaCl) than high salt (1 M NaCl) condition. Considering that the centers of diversity for common bean are different in seed size, the result of Gst statistics showed that bands with relative mobility of 30, 32, 38 and 40 differentiated two weight groups more than other bands. Furthermore, significant differences were observed between these bands for number of pods per plant and number of seeds per plant.

Download Full-text

Evaluation of genetic diversity of wild-type barley (Hordeum vulgare L. sub sp. Spontaneum) based on storage proteins polymorphism

International Journal of Biosciences (IJB) ◽

10.12692/ijb/5.3.266-274 ◽

2014 ◽

Vol 5 (3) ◽

pp. 266-274

Keyword(s):

Genetic Diversity ◽

Hordeum Vulgare ◽

Storage Proteins ◽

Wild Type ◽

Hordeum Vulgare L

Download Full-text

Prolamin variation and evolution in Triticeae as recognized by monoclonal antibodies

Genome ◽

10.1139/g93-139 ◽

1993 ◽

Vol 36 (6) ◽

pp. 1042-1048 ◽

Cited By ~ 8

Author(s):

Susanne Pelger

Keyword(s):

Monoclonal Antibodies ◽

Storage Proteins ◽

Antigenic Site ◽

Seed Storage ◽

Seed Storage Proteins ◽

Specific Site ◽

Hordeum Vulgare L ◽

Triticeae Species ◽

Elymus Species ◽

A Site

The isopropanol-soluble seed storage proteins, prolamins, were studied in 18 different genera of the tribe Triticeae by gel electrophoresis, Coomassie staining, and immunoblot assays. The monoclonal antibodies were originally raised against cultivated barley (Hordeum vulgare L.) hordein, and their reactions had been tested earlier on wild Hordeum species. The study showed that all the investigated Triticeae species produce prolamins and that structural similarities can be found throughout the tribe. The presence of the same antigenic sites in all the species indicates that the polypeptides contain well-conserved regions. They also indicate that the prolamins of the Triticeae species have a common evolutionary origin. In all the investigated species an antigenic site that is common to the B- and C-hordeins of barley was detected. Some of the reacting polypeptides also contained a site that is only present in the B-hordeins. The B-hordein specific site was found in all genera except Agropyron, Hordelymus, and Secale. This shows that although there are similarities between individual polypeptides, the composition of the various prolamin groups may vary between different genera. In the polyploid Elymus species different banding patterns were observed depending on what basic genomes were represented. The results suggest a direct correlation between the presence of a fast migrating polypeptide containing the B-hordein specific site and the presence of the H genome.Key words: Triticeae, prolamin, monoclonal antibodies, evolution, multigene family.

Download Full-text

Genetic diversity in several genotypes of Algerian lentil using biochemical markers

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220659 ◽

2021 ◽

Vol 22 (6) ◽

Author(s):

Hamida Hammadi ◽

DOUNIA HAMMOUDA-BOUSBIA ◽

GHANIA CHAIB ◽

ANFEL TIR

Keyword(s):

Genetic Diversity ◽

Biochemical Markers ◽

Storage Proteins ◽

Economic Value ◽

Seed Storage ◽

Seed Storage Proteins ◽

Grain Protein Content ◽

Protein Profiling ◽

Specific Marker ◽

Pattern Polymorphism

Abstract. Hammadi H, Hammouda-Bousbia D, Chaib G, Tir A. 2021. Genetic diversity in several genotypes of Algerian lentil using biochemical markers. Biodiversitas 22: 3494-2500. Studying seed storage proteins in legumes (Fabaceae) is very important besides its economic value; seed storage proteins play an important role in plant taxonomy because of the stability of their contents. The grain protein content and protein profiles of 12 lentil genotypes (Lens culinaris Medik.) were characterized based on protein profiling through SDS-PAGE. The obtained gel has shown forty bands ranged from 13 to 21 with molecular weight 10 to 120 KDa. Of which six monomorphic bands, thirty-four polymorphic bands and five unique bands in Flip90-31, Idlep3, idlep2, Idlep4 and Radjas genotypes. The protein banding pattern polymorphism (84%) revealed an inter-genotypic variability. The Idlep3 genotype is characterized by specific bands (or a specific marker), followed by the Flip90-31 genotype. So these two genotypes can be considered as valuable gene resources for further breeding programs.

Download Full-text