scholarly journals Genetic Diversity of Common Bean Genotypes as Revealed By Seed Storage Proteins and Some Agronomic Traits

2014 ◽  
Vol 67 (1) ◽  
pp. 125-137 ◽  
Author(s):  
Akbar Marzooghian ◽  
Mohammad Moghaddam ◽  
Mostafa Valizadeh ◽  
Mohammad Hasan Kooshki
Keyword(s):  
Genetic Diversity ◽  
Common Bean ◽  
Yield Components ◽  
Storage Proteins ◽  
Agronomic Traits ◽  
Morphological Characteristics ◽  
Seed Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Seed Morphology

AbstractEvaluation of the genetic diversity present within species is essential for conservation, management and utilization of the genetic resources. The objective of this study was to evaluate genetic variability of 70 common bean genotypes for seed storage proteins, grain morphological characteristics and agronomic traits. Two methods of extracting soluble seed proteins in salt were used.Positive correlations were observed among both seed morphological characters and developmental characters while yield components showed negative correlations with each other. Factor analysis for agronomic and grain morphological traits resulted in three factors were named yield components, seed morphology and phenology, respectively. Most genotypes had lower or medium scores for yield components and phenology factors. Considerable diversity was observed for seed morphology factor among the common bean genotypes.Nei’s diversity coefficient (He= 0.4), effective number of alleles (Ae= 1.69) and number of polymorphic loci (N = 17) indicated larger variation in the extraction method of soluble proteins in low salt (0.2 M NaCl) than high salt (1 M NaCl) condition. Considering that the centers of diversity for common bean are different in seed size, the result of Gst statistics showed that bands with relative mobility of 30, 32, 38 and 40 differentiated two weight groups more than other bands. Furthermore, significant differences were observed between these bands for number of pods per plant and number of seeds per plant.

scholarly journals Genetic variation in common bean (Phaseolus vulgaris L.) using seed protein markers

2020 ◽  
Vol 12 (1) ◽  
pp. 58-69
Author(s):  
Henok Ayelign ◽  
Eleni Shiferaw ◽  
Faris Hailu
Keyword(s):  
Common Bean ◽  
Storage Protein ◽  
Storage Proteins ◽  
Seed Storage Protein ◽  
Gene Diversity ◽  
Seed Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Protein Markers ◽  
Mesoamerican Gene Pool

AbstractThe genetic diversity of common bean accessions were assessed using seed storage protein markers. At regional level, accessions from the two major growing regions showed the highest level of gene diversity (H = 0.322, I = 0.485, and H = 0.312, I = 0.473), which can be exploited for the future improvement of the crop. Based on phaseolin, the major storage protein in common bean, the majority of the accessions (86%) were grouped under Mesoamerican gene pool. Seed proteins were also used to differentiate various Phaseolus species, indicating the usefulness of seed storage proteins in species identification in this genus.

The comparison of genetic diversity between two extraction methods of seed storage proteins in common bean

2011 ◽  
Vol 44 (13) ◽  
pp. S256
Author(s):  
Akbar Marzooghian ◽  
Mostafa Valizadeh ◽  
Mohammad Moghaddam ◽  
Mohammad Hasan Kooshki
Keyword(s):  
Genetic Diversity ◽  
Common Bean ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Extraction Methods

scholarly journals Genetic diversity of red clover cultivars (Trifolium pratense L.) based on protein polimorphism

Genetika ◽  
2010 ◽  
Vol 42 (2) ◽  
pp. 249-258 ◽  
Author(s):  
Zorica Nikolic ◽  
Sanja Vasiljevic ◽  
Djura Karagic ◽  
Milka Vujakovic ◽  
Dusica Jovicic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Storage Proteins ◽  
Genetic Relationships ◽  
Geographic Origin ◽  
Seed Proteins ◽  
Red Clover ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Intellectual Property Right ◽  
Shikimate Dehydrogenase

Red clover is the second most important perennial forage legume. Based on morphological characters alone, it is difficult to distinguish accessories of red clover from each other because they have overlapping variations in terms of the major delimiting morphological and biological characters. The aim of this study was to analyze the genetic relationships of 32 red clover cultivars collected from European gene banks, as well as created in Serbia, based on seed storage proteins and isozymes. From 8 analyzed enzymic systems, there were no differences in zymograms for six enzymes only two enzymes were polymorphic. Two different allelic variants were found for enzyme shikimate dehydrogenase and three for phosphohexose isomeraze. The seed proteins in the area of higher molecular high weight, 55- 95 kDa, were identified as polimorphic. All the cultivars were placed into three clusters on the basis of Ward's distance range. The cluster pattern showed correlation between genetic diversity and geographic origin. Genetic distance between cultivars based on protein fingerprint could be used as a tool to control and protect intellectual property right over plant breeding material.

Methods of Identifying Genetic Diversity in Gliricidia Species for Biomass Production

1993 ◽  
Vol 29 (1) ◽  
pp. 87-96 ◽  
Author(s):  
J. R. Chamberlain ◽  
N. W. Galwey
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rural Communities ◽  
Storage Proteins ◽  
Morphological Characteristics ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Gliricidia Sepium ◽  
Morphological Characters ◽  
The Status

SummaryGliricidia sepium is a leguminous tree native to Mexico and Central America, and is utilized for fuelwood, animal fodder and green manure in rural communities. It is widely distributed throughout the tropics, but many populations of the tree have arisen from haphazard introductions of unknown quality. Methods for determining the genetic structure and origin of populations are therefore required as a basis for genetic improvement. In order to test the effectiveness of various measures of genetic diversity, provenances of G. sepium from Panama, Guatemala, Mexico and Honduras were studied by electrophoresis of seed storage proteins and the enzymes leucine aminopeptidase (LAP) and acid phosphatase (ACP), and by measurement of plant morphological characteristics. Study of seed storage proteins and morphological characters was very informative, and there was some genetic variation for ACP, but LAP showed no clear variation. In the cases where genetic variation was present, the different methods of study showed a consistent pattern of variation: genetic diversity was greatest in the population from Honduras, and least in that from Panama. This was contrary to expectations on the basis of the locations of origin of the populations and the range in which G. sepium is believed to be native. Electrophoresis of the storage proteins of Gliricidia maculata confirmed the status of this species as a distinct taxon.

scholarly journals Mobilization of seed storage proteins is crucial to high vigor in common bean seeds

2022 ◽  
Vol 52 (2) ◽  
Author(s):  
Natalia Carolina Moraes Ehrhardt-Brocardo ◽  
Cileide Maria Medeiros Coelho
Keyword(s):  
Storage Proteins ◽  
Seed Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Seed Vigor ◽  
Intensity Difference ◽  
Biochemical Component ◽  
Germination Process ◽  
Radicle Protrusion ◽  
Relationship Of

ABSTRACT: Seed germination is a complex process controlled by many factors, in which physical and biochemical mechanisms are involved and the mobilization of reserves is crucial for this process to occur. Although, seed reserve mobilization is usually thought to be a post-germination process, seed reserve proteins mobilization occurs during germination. This study quantified seed proteins of bean genotypes during different hydration times, in order to understand the process of protein mobilization and whether there is relationship of this biochemical component with seed vigor. This study was conducted using seeds with different levels of vigor, genotypes with highest (13, 42, 55 and 81) and lowest (07, 23, 44, 50, IPR-88-Uirapurú and Iapar 81) physiological quality. High vigor genotypes showed greater efficiency in hydrolysis and mobilization of protein component, because they presented low globulins content in cotyledons at radicle protrusion in relation to low vigor genotypes (07, 23 and 50). The protein alpha-amylase inhibitor, observed in all genotypes, is involved with the longer time needed for radicle protrusion, according to the band intensity difference in genotypes 07, 44 and Iapar 81.

scholarly journals Genetic diversity in several genotypes of Algerian lentil using biochemical markers

2021 ◽  
Vol 22 (6) ◽  
Author(s):  
Hamida Hammadi ◽  
DOUNIA HAMMOUDA-BOUSBIA ◽  
GHANIA CHAIB ◽  
ANFEL TIR
Keyword(s):  
Genetic Diversity ◽  
Biochemical Markers ◽  
Storage Proteins ◽  
Economic Value ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Grain Protein Content ◽  
Protein Profiling ◽  
Specific Marker ◽  
Pattern Polymorphism

Abstract. Hammadi H, Hammouda-Bousbia D, Chaib G, Tir A. 2021. Genetic diversity in several genotypes of Algerian lentil using biochemical markers. Biodiversitas 22: 3494-2500. Studying seed storage proteins in legumes (Fabaceae) is very important besides its economic value; seed storage proteins play an important role in plant taxonomy because of the stability of their contents. The grain protein content and protein profiles of 12 lentil genotypes (Lens culinaris Medik.) were characterized based on protein profiling through SDS-PAGE. The obtained gel has shown forty bands ranged from 13 to 21 with molecular weight 10 to 120 KDa. Of which six monomorphic bands, thirty-four polymorphic bands and five unique bands in Flip90-31, Idlep3, idlep2, Idlep4 and Radjas genotypes. The protein banding pattern polymorphism (84%) revealed an inter-genotypic variability. The Idlep3 genotype is characterized by specific bands (or a specific marker), followed by the Flip90-31 genotype. So these two genotypes can be considered as valuable gene resources for further breeding programs.

scholarly journals Polymorphism of buckwheat seed storage proteins in cultivar groups, differing by their morphotype

2019 ◽  
Vol 6 (1) ◽  
pp. 10-17
Author(s):  
Ye. Zaika ◽  
N. Kozub ◽  
I. Sozinov ◽  
G. Bidnyk ◽  
P. Karazhbey
Keyword(s):  
Genetic Markers ◽  
Storage Proteins ◽  
Seed Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Breeding Programs ◽  
Cultivar Groups

Aim. To study polymorphism of buckwheat seed proteins in cultivar groups of different morphotypes by the color of corolla. Methods. Electrophoresis by Laemmli method in 17.5 % separating PAAG gel. Results. Electrophoresis spectrum variants with the incidence from 1 to 76 %, common for buckwheat cultivar groups, which are different in their morphotype, were revealed. The obtained results demonstrate a particular level of heterogeneity by the revealed variants in each investigated group of buckwheat genotypes. Variants 2h and 4f, occurring with the incidence of 10 % and 8 % respectively, and specifi c for green-fl owered group of buckwheat cultivars, were also revealed. Conclusions. Different incidence of electrophoresis spectrum components in buckwheat morphotypes, different in fl ower coloring, demonstrates probable different selective value of genes, which control the synthesis of these components or their relation to the genes, closely bound to them. Taking this fact into consideration, buckwheat seed proteins may be molecular and genetic markers for identifi cation of some buckwheat morphotypes, which may be used in breeding programs.

Taxonomic implications of seed morphology and storage proteins in three tribes of the subfamily Papilionoideae (Fabaceae) in Egypt

Phytotaxa ◽  
2021 ◽  
Vol 484 (1) ◽  
pp. 75-95
Author(s):  
MOSTAFA A. ABOULELA ◽  
ZEINAB A. EL-KAREMY ◽  
HASNAA A. HOSNI ◽  
SARA M. SALEH ◽  
AHMED M. FARIED
Keyword(s):  
Multivariate Analyses ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Morphological Characters ◽  
Seed Morphology ◽  
Seed Shape ◽  
The Core ◽  
Phylogenetic Implications ◽  
And Storage

Papilionoideae is the most species-rich subfamily of Fabaceae. Within Papilionoideae, the “core genistoids” comprise many genera of the tribes Podalyrieae, Thermopsideae, Euchresteae, Crotalarieae, Genisteae, and part of Sophoreae. Seed macro- and micro-morphological characters and seed storage proteins of 12 Egyptian species belonging to three tribes of the core genistoides (Sophoreae, Crotalarieae, and Genisteae) were examined using light microscopy, scanning electron microscopy, and SDS-PAGE electrophoresis. Seed shape, size, surface topography, hilum characteristics, epidermal cell patterns, periclinal cell walls, and anticlinal cell boundaries are described here. Seed protein profiles show variable banding patterns with molecular weights ranging from approximately 5 to 270 kDa. Multivariate analyses based on seed morphology and storage proteins elucidate the phenetic relationships among the investigated species at both the tribal and intrageneric levels. The taxonomic and phylogenetic implications of multivariate analyses are compared with previous and current systematic treatments of the genera within the three tribes. Our results confirm the high taxonomic importance of seed morphology in distinguishing among the investigated species. A key to the species using seed macro- and micro-morphological characters is presented.

scholarly journals Engineering sulfur storage in maize seed proteins without apparent yield loss

2017 ◽  
Vol 114 (43) ◽  
pp. 11386-11391 ◽  
Author(s):  
Jose Planta ◽  
Xiaoli Xiang ◽  
Thomas Leustek ◽  
Joachim Messing
Keyword(s):  
Yield Loss ◽  
Storage Proteins ◽  
Seed Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Sulfur Assimilation ◽  
Specific Expression ◽  
Feeding Trials ◽  
The Cost ◽  
Reduction Capacity

Sulfur assimilation may limit the pool of methionine and cysteine available for incorporation into zeins, the major seed storage proteins in maize. This hypothesis was tested by producing transgenic maize with deregulated sulfate reduction capacity achieved through leaf-specific expression of the Escherichia coli enzyme 3′-phosphoadenosine-5′-phosphosulfate reductase (EcPAPR) that resulted in higher methionine accumulation in seeds. The transgenic kernels have higher expression of the methionine-rich 10-kDa δ-zein and total protein sulfur without reduction of other zeins. This overall increase in the expression of the S-rich zeins describes a facet of regulation of these proteins under enhanced sulfur assimilation. Transgenic line PE5 accumulates 57.6% more kernel methionine than the high-methionine inbred line B101. In feeding trials with chicks, PE5 maize promotes significant weight gain compared with nontransgenic kernels. Therefore, increased source strength can improve the nutritional value of maize without apparent yield loss and may significantly reduce the cost of feed supplementation.

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