Background:
Convolvulus species are extensively used in traditional medicines for the
treatment of peptic ulcer diseases primarily associated with the presence of many bioactive compounds,
such as coumarins.
Methods:
A validation and improvement of a rapid, sensitive and simple technique for bioactive compounds
analysis of scopolin and scopoletin in Convolvulus pilosellifolius (CP) and Convolvulus
austroaegyptiacus (CA) plant extracts using UHPLC-MS/MS were performed. Both plants extracts
were subjected to high-resolution DART-ToF-MS technique for fast profiling of their constituents.
Results:
DART-ToF-MS spectra proved the presence of experimental mass at 193.05137 and
355.10371 m/z in the extract of CA plant and at 193.05084 and 355.10365 m/z in CP plant extract for
scopolin and scopoletin compounds, respectively. The total chromatographic analysis time was less
than 10 min with excellent resolution and negligible matrix effect. The validated parameter showed
good linearity (R2
≥0.998) over a wide concentration range of 0.05-10.0 μg mL-1 for both scopolin and
scopoletin, detection limits of scopolin and scopoletin were 0.03 and 0.01 μg mL-1, respectively. The
method also showed good intraday and interday precisions (RSD<4.33%). The recovery percentage
values were between 97.04 and 99.97% at three added concentration limits. The highest content of
scopolin (3.485 mg g-1) and scopoletin (0.795 mg g-1) was detected in CA ethanolic extract. The studied
plants were finally compared with all previous reports in terms of scopolin and scopoletin content.
Conclusion:
The results indicate that the suggested method can be used for regular analysis of active
compounds in medicinal plant extracts.
Development and Validation of an in-House Library of Colombian Candida auris Strains with MALDI-TOF MS to Improve Yeast Identification
