scholarly journals Matrix metalloproteinases (MMP-2 and MMP-9) activity in corneal ulcer and ocular surface disorders determined by gelatin zymography

2012 ◽  
Vol 5 (2) ◽  
pp. 31-35 ◽  
Author(s):  
Arti Singh ◽  
O. P. S. Maurya ◽  
M.V. Jagannadhan ◽  
Ashok Patel
Keyword(s):  
Matrix Metalloproteinases ◽  
Ocular Surface ◽  
Corneal Ulcer ◽  
Gelatin Zymography

scholarly journals Contributions of Ocular Surface Components to Matrix-Metalloproteinases (MMP)-2 and MMP-9 in Feline Tears following Corneal Epithelial Wounding

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e71948 ◽  
Author(s):  
Andrea Petznick ◽  
Michele C. Madigan ◽  
Qian Garrett ◽  
Deborah F. Sweeney ◽  
Margaret D. M. Evans
Keyword(s):  
Matrix Metalloproteinases ◽  
Ocular Surface ◽  
Corneal Epithelial

scholarly journals Kallikrein-Related Peptidase 14 Activates Zymogens of Membrane Type Matrix Metalloproteinases (MT-MMPs)—A CleavEx Based Analysis

2020 ◽  
Vol 21 (12) ◽  
pp. 4383
Author(s):  
Katherine Falkowski ◽  
Ewa Bielecka ◽  
Ida B. Thøgersen ◽  
Oliwia Bocheńska ◽  
Karolina Płaza ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Gelatin Zymography ◽  
Amino Acid Sequences ◽  
Initial Assessment ◽  
Edman Degradation ◽  
Activation Domain ◽  
Additional Layer ◽  
Murine Fibroblasts ◽  
Membrane Type

Kallikrein-related peptidases (KLKs) and matrix metalloproteinases (MMPs) are secretory proteinases known to proteolytically process components of the extracellular matrix, modulating the pericellular environment in physiology and in pathologies. The interconnection between these families remains elusive. To assess the cross-activation of these families, we developed a peptide, fusion protein-based exposition system (Cleavage of exposed amino acid sequences, CleavEx) aiming at investigating the potential of KLK14 to recognize and hydrolyze proMMP sequences. Initial assessment identified ten MMP activation domain sequences which were validated by Edman degradation. The analysis revealed that membrane-type MMPs (MT-MMPs) are targeted by KLK14 for activation. Correspondingly, proMMP14-17 were investigated in vitro and found to be effectively processed by KLK14. Again, the expected neo-N-termini of the activated MT-MMPs was confirmed by Edman degradation. The effectiveness of proMMP activation was analyzed by gelatin zymography, confirming the release of fully active, mature MT-MMPs upon KLK14 treatment. Lastly, MMP14 was shown to be processed on the cell surface by KLK14 using murine fibroblasts overexpressing human MMP14. Herein, we propose KLK14-mediated selective activation of cell-membrane located MT-MMPs as an additional layer of their regulation. As both, KLKs and MT-MMPs, are implicated in cancer, their cross-activation may constitute an important factor in tumor progression and metastasis.

Immunohistochemical and gelatin zymography studies for matrix metalloproteinases in bleomycin-induced pulmonary fibrosis

1998 ◽  
Vol 48 (12) ◽  
pp. 954-963 ◽  
Author(s):  
Takamoto Yaguchi ◽  
Yuh Fukuda ◽  
Masamlchi Ishizaki ◽  
Nobuaki Yamanaka
Keyword(s):  
Matrix Metalloproteinases ◽  
Pulmonary Fibrosis ◽  
Gelatin Zymography

scholarly journals Repression of Matrix Metalloproteinases and Cytokine Secretion in Glioblastoma by Targeting K+ Channel: An in Vitro Study

2021 ◽  
Author(s):  
Farshid Saadat ◽  
◽  
Zohreh Zareighane ◽  
Farnaz Safavifar ◽  
Seyedeh Zohreh Jalali ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Cell Viability ◽  
Malignant Tumors ◽  
Cell Model ◽  
Gelatin Zymography ◽  
In Vitro Study ◽  
K Channel ◽  
Kv Channels ◽  
Vitro Model

Introduction: Glioblastoma is an aggressive malignancy of human brain with poorly understood pathogenesis. Voltage-gated potassium (Kv) channels and Matrix metalloproteinases (MMPs) are highly expressed in malignant tumors and involved in the progression and metastasis of glioblastoma. The purpose of this study was to determine whether a voltage-dependent potassium channel blocker could modulate astrocytes as a cell which involved in immunopathogenesis of glioblastoma. Methods: The cytotoxic effect of 4-aminopyridine (4-AP) at different doses in cell model of glioblastoma was measured by MTT assay. ELISA technique and gelatin zymography were used to assess cytokines levels and MMP-9 after 4-AP treatment, respectively. Results: Cytotoxicity analysis showed that cell viability reduced by increasing 4-AP level and cell growth reduced gradually by removing 4-AP from cell medium. 4-AP inhibits secretion of IL-6 and IL-1 (p<0.05). MMP9 activity significantly inhibits with increased 4-AP dose as compared to non-treated cells. Conclusion: Reduction of cell viability, IL-6 secretion and MMP-9 activity in an in vitro model of glioblastoma, might be assumed 4-AP as an agent for chemoprevention of cancer.

scholarly journals Blowout fracture-associated orbital cellulitis progressing to panophthalmitis: a case report

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Atsuhide Takesue ◽  
Yosuke Asada ◽  
Hiroki Ooya ◽  
Toshiyuki Yokoyama
Keyword(s):  
Ocular Surface ◽  
Corneal Ulcer ◽  
Chronic Sinusitis ◽  
Orbital Cellulitis ◽  
Blowout Fracture ◽  
Obligate Anaerobic ◽  
Parvimonas Micra ◽  
Chronic Periodontal Disease ◽  
The Right ◽  
Head Computed Tomography

Abstract Background Parvimonas micra is known as a causative agent of chronic periodontal disease. This Gram-positive obligate anaerobic coccus was cultured from the ocular surface of blowout fracture-related orbital cellulitis progressing to panophthalmitis. Case presentation The patient was a woman in her fifties who had panic disorder and subsequently was a victim of domestic violence. These factors led to delayed consultation. At the initial visit to an ophthalmologist, the ocular surface of the right eye was covered with pus. Swelling of the upper and lower eyelids prevented the eyelid from closing and exophthalmos, severe corneal ulcer, panophthalmitis, and no light perception were observed. Head computed tomography revealed an old blowout fracture and chronic sinusitis with orbital cellulitis. P. micra were isolated from culture of pus samples from the sinus and from the ocular surface. Conclusions There is a possibility that P. micra invaded the orbit via the fragile bony site and caused orbital cellulitis, severe corneal ulcer, and panophthalmitis that required enucleation. In cases of coexisting old blowout fracture and chronic sinusitis, the chronic sinusitis should be treated as quickly as possible.

226. Expression of matrix metalloproteinases in bovine thecal cells

2005 ◽  
Vol 17 (9) ◽  
pp. 88
Author(s):  
L. Harland ◽  
H. F. Irving-Rodgers ◽  
S. E. Morris ◽  
R. J. Rodgers
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinases ◽  
Phorbol Ester ◽  
Granulosa Cells ◽  
Gelatin Zymography ◽  
Mrna Levels ◽  
Cell Culture Systems ◽  
Thecal Cells ◽  
Theca Interna ◽  
Free Media

As follicles grow the thecal layers expand. It is likely that extracellular matrix is remodelled in this process and possibly by matrix metalloproteinases (MMPs). A promising candidate to regulate MMPs is insulin-like factor 3 (INSL3). It is produced by thecal cells, its receptor, LGR8, is expressed in the theca interna (unpublished) and a related molecule, relaxin, regulates turnover of matrix in a number of tissues. For this reason we sort to examine the role of INSL3 in matrix turnover. However, in all thecal cell culture systems examined LGR8 receptors appear to be down regulated within 24 h. We therefore examined the effects of second messenger pathway activators. Thecal and granulosa cells were isolated and cultured and the levels of RNA for MMP2 and 9 quantitated by RT-PCR. MMP2 mRNA levels in thecal tissues were >10 fold higher than in granulosa cells (n = 19 follicles >10 mm). MMP2 levels were substantially greater than MMP9. At 12 h phorbol ester (100 nM phorbol 12,13-didecanoate) increased thecal expression of MMP 9 mRNA levels 11.5 fold (P < 0.001) and at 48 h MMP2 mRNA was increased 5 fold (P < 0.01). Pieces of whole follicle wall [follicles <5 mm in diameter, classified as healthy (n = 12) or atretic (n = 6)] were cultured in serum free media. Expression of the steroidogenic enzymes 17β HSD and P450scc but not 3β HSD were detected by immunohistochemistry even after 10 days. MMP activity on day 2 was analysed by gelatin zymography. Treatment with phorbol ester increased active MMP9 19 fold (P < 0.001). Treatment of thecal cells or follicle walls with 1 mM dibutyryl cAMP induced additional MMP activities at sizes of 110 and 122kDa. No effects on MMP2 activity were observed. In conclusion whilst we do not know the ligand inducers of the synthesis and activator of MMPs in thecal cells they can be regulated. Hence MMPs are candidates for remodelling the extracellular matrix of thecal layers.

scholarly journals Alterations in the Ocular Surface Microbiome in Traumatic Corneal Ulcer Patients

2020 ◽  
Vol 61 (6) ◽  
pp. 35 ◽  
Author(s):  
Yutong Kang ◽  
Hao Zhang ◽  
Meina Hu ◽  
Yao Ma ◽  
Pengfei Chen ◽  
...  
Keyword(s):  
Ocular Surface ◽  
Corneal Ulcer

scholarly journals Expression of Matrix Metalloproteinases Subsequent to Urogenital Chlamydia muridarum Infection of Mice

2005 ◽  
Vol 73 (10) ◽  
pp. 6962-6973 ◽  
Author(s):  
K.H. Ramsey ◽  
I.M. Sigar ◽  
J. H. Schripsema ◽  
N. Shaba ◽  
K. P. Cohoon
Keyword(s):  
Matrix Metalloproteinases ◽  
Gelatin Zymography ◽  
Mouse Strains ◽  
Post Translational Modification ◽  
Chlamydia Muridarum ◽  
Central Hypothesis ◽  
Casein Zymography ◽  
Increased Activity ◽  
Mmp Genes ◽  
Chronic Sequelae

ABSTRACT The central hypothesis of this study was that matrix metalloproteinases (MMPs) would be enhanced following murine chlamydial infection and that their expression would vary in mouse strains that differ in their susceptibility to chronic chlamydia-induced disease. To address this hypothesis, female C3H/HeN and C57BL/6 mice were infected intravaginally with Chlamydia muridarum. Uterine and oviduct tissues were assessed for transcription of MMP genes and their tissue inhibitors. An increased activity of MMP genes relative to preinfection tissues was observed in the C3H/HeN mice when compared to C57BL/6 mice. Using gelatin zymography, we detected constitutive MMP-2 activity in both strains of mice but an increase in MMP-9. Casein zymography indicated the presence of two elastase-like activities consistent with MMP-12 and possibly MMP-7. Western blotting and antigen capture enzyme-linked immunoassay also confirmed an increase in MMP-9 but constitutive MMP-2 expression subsequent to the infection in both strains of mice. In C57BL/6 mice, MMP-9 was present in monomer and dimer form throughout the 56-day monitoring period. C3H/HeN mice produced dimeric MMP-9, but increases in the monomer form were also observed through day 14. Post-translational modification of MMP-9 between the two strains also differed. Immunohistochemistry revealed neutrophils as a prominent source for MMP-9 in both strains of mice. We conclude that differences in the relative expression and activity of MMPs, particularly MMP-9, occur in mice differing in their susceptibility to the development of chronic chlamydial disease. These differences may account for disparate outcomes with regard to chronic sequelae of the disease.

scholarly journals Amniotic Membrane Graft in Ocular Cicatricial Pemphigus: A More Conservative Approach

2020 ◽  
pp. 1-4
Author(s):  
Fouad Chraibi
Keyword(s):  
Case Report ◽  
Amniotic Membrane ◽  
Conservative Management ◽  
Ulcer Healing ◽  
Ocular Surface ◽  
Corneal Ulcer ◽  
Immunosuppressive Treatment ◽  
Conservative Approach ◽  
Newly Diagnosed ◽  
Adnexal Surgery

Purpose: To report more conservative management of ocular cicatricial pemphigus (OCP) lesions by performing an initial corneal partial amniotic membrane graft without disturbance of the inflamed conjunctiva. Methods: A case report of a patient having a stage 3 OCP in his left eye with a corneal ulcer. He was managed by partial corneal amniotic membrane (AM) graft. Results: The course was favorable with complete corneal ulcer healing and incidentally there was an important regression of associated ocular surface inflammatory signs with a less prominent inferior symblepharon strand. Conclusion: We propose to manage newly diagnosed patients having advanced ocular cicatricial pemphigus disease with partial corneal amniotic membrane graft before performing radical adnexal surgery with prior immunosuppressive treatment.

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