Myelomonocytic leukemia with intracytoplasmic crystalline inclusions, double minute chromosomes and MYC amplification

2017 ◽  
Vol 106 (4) ◽  
pp. 457-458
Author(s):  
Julia T. Geyer ◽  
Susan Mathew
Keyword(s):  
Crystalline Inclusions ◽  
Double Minute ◽  
Myelomonocytic Leukemia ◽  
Double Minute Chromosomes

scholarly journals FLT3 Amplification as Double Minute Chromosomes in a Patient with Chronic Myelomonocytic Leukemia

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Heyang Zhang ◽  
Xiaoxue Wang ◽  
Shibo Li ◽  
Xianfu Wang ◽  
Xianglan Lu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Gene Amplification ◽  
Chronic Myelomonocytic Leukemia ◽  
Comparative Genomic ◽  
Myeloid Neoplasms ◽  
Double Minute ◽  
Myelomonocytic Leukemia ◽  
Double Minute Chromosomes

Double minute chromosomes (dmins) are a form of gene amplification presenting as small spherical paired chromatin bodies. Dmins are rare in hematologic malignancies and are generally associated with a poor prognosis. Some case reports identified MYC or MLL gene amplification performing as dmin in myeloid neoplasms. FLT3 (FMS-related tyrosine kinase 3) acts as an oncogene in myeloid neoplasms which is associated with several signal transduction pathways. Genomic amplification of FLT3 has not been reported in hematological disease. The current study attempts to demonstrate the existence of double minute chromosomes via FLT3 gene amplification in a patient diagnosed with chronic myelomonocytic leukemia (CMML). Routine G-banded karyotype, array-based comparative genomic hybridization, and fluorescence in situ hybridization analyses were used to characterize the cytogenetic abnormality in the patient’s bone marrow. FLT3 amplification as dmins in a patient with CMML was revealed. This case study reports a rare double minute chromosome via FLT3 amplification in CMML by using array-based comparative genomic hybridization and fluorescence in situ hybridization analyses. The study also proposed another possible mechanism of FLT3 genes in leukemogenesis.

Trisomy 6 and Double Minute Chromosomes in a Case of Chronic Myelomonocytic Leukemia

1998 ◽  
Vol 106 (2) ◽  
pp. 180-181 ◽  
Author(s):  
Constantina Sambani ◽  
Dimitris T.P. Trafalis ◽  
George Vessalas ◽  
George Politis ◽  
Platon Peristeris ◽  
...  
Keyword(s):  
Chronic Myelomonocytic Leukemia ◽  
Double Minute ◽  
Myelomonocytic Leukemia ◽  
Double Minute Chromosomes

Acute myelomonocytic leukemia with double minute chromosomes and a normal karyotype

1987 ◽  
Vol 25 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Junko H. Ohyashiki ◽  
Kazuma Ohyashiki ◽  
Kenneth B. Miller ◽  
Barry P. Cuiffo ◽  
Avery A. Sandberg
Keyword(s):  
Normal Karyotype ◽  
Double Minute ◽  
Acute Myelomonocytic Leukemia ◽  
Myelomonocytic Leukemia ◽  
Double Minute Chromosomes

Induction of metallothionein-I mRNA in cultured cells by heavy metals and iodoacetate: evidence for gratuitous inducers

1984 ◽  
Vol 4 (3) ◽  
pp. 484-491
Author(s):  
D M Durnam ◽  
R D Palmiter
Keyword(s):  
Heavy Metals ◽  
Cultured Cells ◽  
Mrna Accumulation ◽  
Mouse Hepatocyte ◽  
Hepatocyte Cell Line ◽  
Regulatory Molecule ◽  
Heavy Metal Detoxification ◽  
Double Minute ◽  
Double Minute Chromosomes

A mouse hepatocyte cell line selected for growth in 80 microM CdSO4 (Cdr80 cells) was used to test the role of metallothioneins in heavy metal detoxification. The cadmium-resistant (Cdr80) cells have double minute chromosomes carrying amplified copies of the metallothionein-I gene and accumulate ca. 20-fold more metallothionein-I mRNA than unselected cadmium-sensitive (Cds) cells after optimal Cd stimulation. As a consequence, the amount of Cd which inhibits DNA synthesis by 50% is ca. 7.5-fold higher in Cdr80 cells than in Cds cells. Cds and Cdr80 cells were compared in terms of their resistance to other heavy metals. The results indicate that although Zn, Cu, Hg, Ag, Co, Ni, and Bi induce metallothionein-I mRNA accumulation in both Cdr80 and Cds cells, the Cdr80 cells show increased resistance to only a subset of these metals (Zn, Cu, Hg, and Bi). This suggests that not all metals which induce metallothionein mRNA are detoxified by metallothionein and argues against autoregulation of metallothionein genes. Metallothionein-I mRNA is also induced by iodoacetate, suggesting that the regulatory molecule has sensitive sulfhydryl groups.

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