Antibiotic resistance and molecular characterization of clinical isolates of methicillin-resistant coagulase-negative staphylococci isolated from bacteremic patients in oncohematology

2011 ◽  
Vol 56 (2) ◽  
pp. 122-130 ◽  
Author(s):  
O. Bouchami ◽  
W. Achour ◽  
M. A. Mekni ◽  
J. Rolo ◽  
A. Ben Hassen
Keyword(s):  
Antibiotic Resistance ◽  
Molecular Characterization ◽  
Clinical Isolates ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant

Molecular Characterization of Methicillin-Resistant Coagulase-Negative Staphylococci from a Neonatal Intensive Care Unit

2002 ◽  
Vol 23 (8) ◽  
pp. 447-451 ◽  
Author(s):  
Isabel Bogado ◽  
Adriana Limansky ◽  
Emma Sutich ◽  
Patricia Marchiaro ◽  
Marta Marzi ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Molecular Characterization ◽  
Neonatal Intensive Care ◽  
Genomic Diversity ◽  
Phenotypic Characterization ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant

Objective:To evaluate clonal dissemination of methicillin-resistant coagulase-negative staphylococci (CNS).Setting:Neonatal intensive care unit of a 180-bed, university-affiliated general hospital.Patients:Neonates admitted to the neonatal intensive care unit between March 1999 and October 2000, from whom CNS were isolated as a unique pathogen. Patients from other wards from whom epidemiologically unrelated staphylococci strains were obtained served as control-patients.Methods:Conventional methods were used for phenotypic characterization of CNS. Methicillin resistance was determined bymecA polymerase chain reaction (PCR) amplification. Genotypic characterization was done by random amplification of DNA with degenerated primers (RAPD) and repetitive element sequence-based PCR (rep-PCR).Results:Forty methicillin-resistant CNS isolates obtained from neonates were characterized asStaphylococcus epidermidis(33),S. hominis(5),S. warneri(1), andS. auricularis(1). Both RAPD and rep-PCR indicated the presence of 4 different clones among the 33S. epidermidisisolates. In turn, the 4 randomly selected, epidemiologically unrelated methicillin-resistant CNS strains obtained from control-patients showed 3 new profiles by RAPD and 2 by rep-PCR, which differed from the corresponding patterns mentioned earlier. Persistence ofS. hominisin a neonate could be assessed by both genotypic techniques.Conclusions:The molecular characterization of the methicillin-resistant CNS studied indicated dissemination of one particular methicillin-resistant CNS clone among the neonates in the ward studied. Although RAPD showed a superior power to discriminate among methicillin-resistant CNS isolates, both RAPD and rep-PCR detected intraspecific and interspecific genomic diversity.

scholarly journals Susceptibility and molecular characterization of mec A- and mec B-positive community acquired methicillin-resistant Staphylococcus aureus isolates from students

2021 ◽  
Vol 18 (2) ◽  
pp. 155-171
Author(s):  
Patrick O. Olorunfemi ◽  
Ndidi C. Ngwuluka ◽  
Josiah A. Onaolapo ◽  
Yakubu K.E. Ibrahim
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Molecular Characterization ◽  
Resistance Genes ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meca Gene ◽  
Public Health Importance ◽  
Methicillin Resistant ◽  
Spa Gene

Staphylococcus aureus is an organism of great public health importance. It is widely studied because it is virulent, causes life threatening disease and has ability to adapt to diverse environmental conditions and so develops resistance to antibiotics easily. As a result, there is a need for surveillance of its antibiotic resistance and resistance genes. The susceptibility and molecular characterization of methicillin resistant Staphylococcus aureus recovered from urine samples of healthy students were undertaken. Standard procedures were employed for isolation, identification, susceptibility, and polymerase chain reaction analyses. Out of 217 samples collected, 73 were confirmed Staphylococcus aureus. Most of the isolates were susceptible to ciprofloxacin and vancomycin followed by gentamicin and co-trimoxazole and least susceptible to penicillin, cefotaxime, ofloxacin and cefoxitin. Thirty-two (32) isolates were resistant to 5 antibiotics while 3 isolates were resistant to the 11 antibiotics used in this study. Sixteen phenotypically methicillin resistant isolates contained mecA gene while ten of the isolates also showed the presence of mecB gene. The characteristic Sa442 and nuc genes of Staphylococcus aureus and the presence of spa gene confirmed MRSA. Continous surveillance for antibiotic resistance and resistance genes is paramount at local, regional and national levels. Surveillance data will assist in implementing interventions. Keywords: Antibiotic resistance; Methicillin-resistant Staphylococcus aureus, mecA, mecB, CA-MRSA; Surveillance

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