WITHDRAWN: Molecular characterization of the dynamics of nasal carriage by methicillin resistant coagulase-negative staphylococci

2006 ◽  
Author(s):  
H. de Lencastre ◽  
I. Couto ◽  
M. Miragaia
Keyword(s):  
Molecular Characterization ◽  
Nasal Carriage ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant

The epidemiology and molecular characterization of methicillin-resistant staphylococci sampled from a healthy Jordanian population

2013 ◽  
Vol 141 (11) ◽  
pp. 2384-2391 ◽  
Author(s):  
A. G. AL-BAKRI ◽  
H. AL-HADITHI ◽  
V. KASABRI ◽  
G. OTHMAN ◽  
A. KRIEGESKORTE ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Nasal Carriage ◽  
Control Measures ◽  
Coagulase Negative Staphylococci ◽  
Carriage Rate ◽  
Methicillin Resistant ◽  
Infection Control Measures ◽  
Type Iv ◽  
Encoding Genes

SUMMARYThe prevalence of natural carriage and molecular epidemiology of methicillin-resistantStaphylococcus aureus(MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolates in a Jordanian community were investigated. The MRSA nasal carriage rate in 227 healthy volunteers was 7·5% and the majority (81%) of MRSA harboured the resistance element SCCmectype IVe and were of a novelspatype t9519 (76%); other significantspagene types were t223 (14·7%) and t044 (5·9%). All MRSA isolates were susceptible to other classes of antibiotics, and tested positive for at least three virulence factor encoding genes, but only two harboured thepvlgene. MR-CoNS carriage was 54·2% and these isolates were characterized by single, double and untypable SCCmecelements, withStaphylococcus epidermidisSCCmectype IVa predominating. Of eight subjects with nasal co-colonization of MR-CoNS + MRSA, three shared SCCmectype IV in both groups of organisms. This is the first report of methicillin-resistant staphylococci carriage in a Jordanian community and its findings are important for epidemiological study and infection control measures of these organisms.

Molecular Characterization of Methicillin-Resistant Coagulase-Negative Staphylococci from a Neonatal Intensive Care Unit

2002 ◽  
Vol 23 (8) ◽  
pp. 447-451 ◽  
Author(s):  
Isabel Bogado ◽  
Adriana Limansky ◽  
Emma Sutich ◽  
Patricia Marchiaro ◽  
Marta Marzi ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Molecular Characterization ◽  
Neonatal Intensive Care ◽  
Genomic Diversity ◽  
Phenotypic Characterization ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant

Objective:To evaluate clonal dissemination of methicillin-resistant coagulase-negative staphylococci (CNS).Setting:Neonatal intensive care unit of a 180-bed, university-affiliated general hospital.Patients:Neonates admitted to the neonatal intensive care unit between March 1999 and October 2000, from whom CNS were isolated as a unique pathogen. Patients from other wards from whom epidemiologically unrelated staphylococci strains were obtained served as control-patients.Methods:Conventional methods were used for phenotypic characterization of CNS. Methicillin resistance was determined bymecA polymerase chain reaction (PCR) amplification. Genotypic characterization was done by random amplification of DNA with degenerated primers (RAPD) and repetitive element sequence-based PCR (rep-PCR).Results:Forty methicillin-resistant CNS isolates obtained from neonates were characterized asStaphylococcus epidermidis(33),S. hominis(5),S. warneri(1), andS. auricularis(1). Both RAPD and rep-PCR indicated the presence of 4 different clones among the 33S. epidermidisisolates. In turn, the 4 randomly selected, epidemiologically unrelated methicillin-resistant CNS strains obtained from control-patients showed 3 new profiles by RAPD and 2 by rep-PCR, which differed from the corresponding patterns mentioned earlier. Persistence ofS. hominisin a neonate could be assessed by both genotypic techniques.Conclusions:The molecular characterization of the methicillin-resistant CNS studied indicated dissemination of one particular methicillin-resistant CNS clone among the neonates in the ward studied. Although RAPD showed a superior power to discriminate among methicillin-resistant CNS isolates, both RAPD and rep-PCR detected intraspecific and interspecific genomic diversity.

scholarly journals Molecular characterization of the first community-acquired methicillin-resistant Staphylococcus aureus strains from Central Iran

2013 ◽  
Vol 17 (11) ◽  
pp. e949-e954 ◽  
Author(s):  
Alireza Japoni-Nejad ◽  
Mohsen Rezazadeh ◽  
Hamid Kazemian ◽  
Nasimeh Fardmousavi ◽  
Alex van Belkum ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Central Iran ◽  
Methicillin Resistant

scholarly journals Molecular characterization of spa type t127, sequence type 1 methicillin-resistant Staphylococcus aureus from pigs

2011 ◽  
Vol 66 (6) ◽  
pp. 1231-1235 ◽  
Author(s):  
A. Franco ◽  
H. Hasman ◽  
M. Iurescia ◽  
R. Lorenzetti ◽  
M. Stegger ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sequence Type ◽  
Methicillin Resistant ◽  
Spa Type
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