Effect of genomic rearrangement on heavy metal tolerance in the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp245

2011 ◽  
Vol 57 (1) ◽  
pp. 5-10 ◽  
Author(s):  
Andrei V. Shelud’ko ◽  
Olga E. Varshalomidze ◽  
Lilia P. Petrova ◽  
Elena I. Katsy
Keyword(s):  
Heavy Metal ◽  
Plant Growth ◽  
Azospirillum Brasilense ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Genomic Rearrangement ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Plant Growth-Promoting Rhizobacteria Isolated from the Jujube (Ziziphus lotus) Plant Enhance Wheat Growth, Zn Uptake, and Heavy Metal Tolerance

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 316
Author(s):  
Nidal Fahsi ◽  
Ismail Mahdi ◽  
Abdelhalem Mesfioui ◽  
Latefa Biskri ◽  
Abdelmounaaim Allaoui
Keyword(s):  
Plant Growth ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Wheat Plant ◽  
Plant Growth Promoting Rhizobacteria ◽  
Wheat Seedling ◽  
Phosphate Solubilizing Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

In recent years, the low Zn content of wheat has become critical. Consequently, solutions that can improve the Zn nutrition of wheat are highly researched. In the present investigation, we aimed to evaluate the potential benefits of phosphate-solubilizing bacteria isolated from Ziziphus lotus on wheat seedling growth. Based on the phosphate-solubilizing criteria, four isolated strains, J16, J143, J146, and J158, were identified by 16SrRNA gene sequencing as Pseudomonas moraviensis, Bacillus halotolerans, Enterobacter hormaechei, and Pseudomonas frederiksbergensis, respectively. Studies of the conventional properties of plant growth-promoting rhizobacteria (PGPR) showed that E. hormaechei J146 produced up to 550 mg·L−1 of indole-3-acetic acid (IAA). Siderophores and ammonia were produced by all strains but cellulase was restricted to B. halotolerans J143, whereas proteases were missing in E. hormaechei J146 and P. frederiksbergensis J158. E. hormaechei J146 tolerate up to 1.5 mg·L−1 of copper and cadmium, while B. halotolerans J143 withstood 1.5 mg·L−1 of nickel. Strains B. halotolerant J143, E. hormaechei J146, and P. frederiksbergensis J158 remarkably improved wheat seed germination, plant growth, and Zn absorption. Lastly, nutrient measurement revealed that a wheat plant inoculated with E. hormaechei J146 and P. frederiksbergensis J158 increased its nitrogen and potassium uptake by up to 17%.

scholarly journals Alleviation of Salinity and Metal Stress Using Plant Growth-Promoting Rhizobacteria Isolated From Semiarid Moroccan Copper-Mine Soils

2021 ◽  
Author(s):  
Atika Madline ◽  
Leila Benidire ◽  
Ali Boularbah
Keyword(s):  
Heavy Metal ◽  
Plant Growth ◽  
Root Elongation ◽  
Metal Tolerance ◽  
Soluble Sugars ◽  
Plant Growth Promoting Rhizobacteria ◽  
Metal Stress ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Phytoremediation is an eco-friendly method for rehabilitation of mine tailing using plants and their associated rhizosphere microorganisms. Some heavy metal and salt-tolerant plant growth promoting rhizobacteria (PGPR) could be beneficial in alleviating soil salinity and heavy metal stress. The aim of this work is to select PGPR that could be used in phytoremediation process. Twenty-nine rhizobacteria were examined for their ability to grow at increasing concentrations of NaCl and high concentrations of Zn, Pb, Cu and Cd. The results showed that seventeen rhizobacteria displayed high salinity and metal tolerance (up to 100g L− 1 NaCl, up to 5 mM Cd, 9 mM Pb, 10 mM Zn, and Cu up to 6 mM). This work showed also that salinity and metallic stress has affected bacterial growth and metabolism by increasing intracellular proline, soluble sugars, free amino-acids and exopolysaccharides production. Moreover, almost all tested bacteria maintained their PGP traits under 10 % of NaCl and multi-metal stress. Four strains exhibiting the best PGP activities namely Mesorhizobium tamadayense, Enterobacter xiangfangensis, Pseudomonas azotifigens and Streptomyces Caelestis were selected for root elongation bioassay. The consortium of these rhizobacteria improves significantly the root elongation of Peganum harmala and Lactuca sativa under metallic and salt stress. Thus, the rhizobacteria with beneficial traits as well as tolerance to abiotic stress could be useful to stimulate plants establishment under different environmental stresses.

Phytormediation Efficiency Increased by Using Plant Growth Promoting Bacteria (PGPB) and Chelates

2020 ◽  
pp. 85-103
Keyword(s):  
Heavy Metal ◽  
Plant Growth ◽  
Metal Contamination ◽  
Chelating Agents ◽  
Heavy Metal Contamination ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

In this chapter, the authors give information about the plant-growth-promoting bacteria and chelating agents removing high number of contaminants with the help of phytoremdiation technology. To the best of the authors' knowledge, this is the first chapter about heavy metal contamination in groundwater and soil removing by microbes and chelates.

Ultrastructure of interaction in alginate beads between the microalgaChlorella vulgariswith its natural associative bacteriumPhyllobacterium myrsinacearumand with the plant growth-promoting bacteriumAzospirillum brasilense

2001 ◽  
Vol 47 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Vladimir K Lebsky ◽  
Luz E Gonzalez-Bashan ◽  
Yoav Bashan
Keyword(s):  
Wastewater Treatment ◽  
Plant Growth ◽  
Azospirillum Brasilense ◽  
Bacterial Species ◽  
Alginate Beads ◽  
Transmission Electron ◽  
Associative Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Treatment Water

Chlorella vulgaris, a microalga often used in wastewater treatment, was coimmobilized and coincubated either with the plant growth-promoting bacterium Azospirillum brasilense, or with its natural associative bacterium Phyllobacterium myrsinacearum, in alginate beads designed for advanced wastewater treatment. Interactions between the microalga and each of the bacterial species were followed using transmission electron microscopy for 10 days. Initially, most of the small cavities within the beads were colonized by microcolonies of only one microorganism, regardless of the bacterial species cocultured with the microalga. Subsequently, the bacterial and microalgal microcolonies merged to form large, mixed colonies within the cavities. At this stage, the effect of bacterial association with the microalga differed depending on the bacterium present. Though the microalga entered a senescence phase in the presence of P. myrsinacearum, it remained in a growth phase in the presence of A. brasilense. This study suggests that there are commensal interactions between the microalga and the two plant associative bacteria, and that with time the bacterial species determined whether the outcome for the microalga is senescence or continuous multiplication.Key words: Azospirillum, Chlorella, Phyllobacterium, wastewater treatment, water bioremediation.

scholarly journals Protection of Tomato Seedlings against Infection by Pseudomonas syringae pv. Tomato by Using the Plant Growth-Promoting Bacterium Azospirillum brasilense

2002 ◽  
Vol 68 (6) ◽  
pp. 2637-2643 ◽  
Author(s):  
Yoav Bashan ◽  
Luz E. de-Bashan
Keyword(s):  
Plant Growth ◽  
Pseudomonas Syringae ◽  
Azospirillum Brasilense ◽  
Dry Weight ◽  
Tomato Plants ◽  
Bacterial Speck ◽  
Plant Growth Promoting ◽  
Bacterial Speck Disease ◽  
Growth Promoting ◽  
Mist Chamber

ABSTRACT Pseudomonas syringae pv. tomato, the causal agent of bacterial speck of tomato, and the plant growth-promoting bacterium Azospirillum brasilense were inoculated onto tomato plants, either alone, as a mixed culture, or consecutively. The population dynamics in the rhizosphere and foliage, the development of bacterial speck disease, and their effects on plant growth were monitored. When inoculated onto separate plants, the A. brasilense population in the rhizosphere of tomato plants was 2 orders of magnitude greater than the population of P. syringae pv. tomato (107 versus 105 CFU/g [dry weight] of root). Under mist chamber conditions, the leaf population of P. syringae pv. tomato was 1 order of magnitude greater than that of A. brasilense (107 versus 106 CFU/g [dry weight] of leaf). Inoculation of seeds with a mixed culture of the two bacterial strains resulted in a reduction of the pathogen population in the rhizosphere, an increase in the A. brasilense population, the prevention of bacterial speck disease development, and improved plant growth. Inoculation of leaves with the mixed bacterial culture under mist conditions significantly reduced the P. syringae pv. tomato population and significantly decreased disease severity. Challenge with P. syringae pv. tomato after A. brasilense was established in the leaves further reduced both the population of P. syringae pv. tomato and disease severity and significantly enhanced plant development. Both bacteria maintained a large population in the rhizosphere for 45 days when each was inoculated separately onto tomato seeds (105 to 106 CFU/g [dry weight] of root). However, P. syringae pv. tomato did not survive in the rhizosphere in the presence of A. brasilense. Foliar inoculation of A. brasilense after P. syringae pv. tomato was established on the leaves did not alleviate bacterial speck disease, and A. brasilense did not survive well in the phyllosphere under these conditions, even in a mist chamber. Several applications of a low concentration of buffered malic acid significantly enhanced the leaf population of A. brasilense (>108 CFU/g [dry weight] of leaf), decreased the population of P. syringae pv. tomato to almost undetectable levels, almost eliminated disease development, and improved plant growth to the level of uninoculated healthy control plants. Based on our results, we propose that A. brasilense be used in prevention programs to combat the foliar bacterial speck disease caused by P. syringae pv. tomato.

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