Mineral Oil Inhibits Movement of Potato Virus Y in Potato Plants in an Age-Dependent Manner

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

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Role of the methionine cycle in the temperature‐sensitive responses of potato plants to potato virus Y

Molecular Plant Pathology ◽

10.1111/mpp.13009 ◽

2020 ◽

Vol 22 (1) ◽

pp. 77-91 ◽

Cited By ~ 1

Author(s):

Igor Fesenko ◽

Nadezhda Spechenkova ◽

Anna Mamaeva ◽

Antonida V. Makhotenko ◽

Andrew J. Love ◽

...

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Temperature Sensitive ◽

Potato Plants ◽

Methionine Cycle

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Possible induction of potato plant defences against Potato virus Y by mineral oil application

European Journal of Plant Pathology ◽

10.1007/s10658-016-1006-7 ◽

2016 ◽

Vol 147 (2) ◽

pp. 339-348 ◽

Cited By ~ 3

Author(s):

M. Khelifa

Keyword(s):

Potato Virus ◽

Potato Plant ◽

Potato Virus Y ◽

Mineral Oil ◽

Plant Defences

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Expression of genes for resistance to potato virus Y in potato plants and protoplasts

Annals of Applied Biology ◽

10.1111/j.1744-7348.1984.tb03080.x ◽

1984 ◽

Vol 105 (3) ◽

pp. 539-545 ◽

Cited By ~ 38

Author(s):

H. BARKER ◽

B. D. HARRISON

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Potato Plants ◽

Expression Of Genes

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Salicylic Acid Is Involved in the Nb-Mediated Defense Responses to Potato virus X in Solanum tuberosum

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-23-4-0394 ◽

2010 ◽

Vol 23 (4) ◽

pp. 394-405 ◽

Cited By ~ 29

Author(s):

Gerardo Sánchez ◽

Nadia Gerhardt ◽

Florencia Siciliano ◽

Adrián Vojnov ◽

Isabelle Malcuit ◽

...

Keyword(s):

Salicylic Acid ◽

Solanum Tuberosum ◽

Potato Virus ◽

Potato Virus X ◽

Defense Responses ◽

Avirulent Strain ◽

Dependent Manner ◽

Hypersensitive Resistance ◽

Potato Plants ◽

Transgenic Lines

To evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species–generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the avirulent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-1a in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hypersensitive resistance to PVX.

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Role of Hairy Nightshade in the Transmission of Different Potato virus Y Strains on Solanum tuberosum (L.)

Plant Health Progress ◽

10.1094/php-2010-0526-05-rs ◽

2010 ◽

Vol 11 (1) ◽

pp. 38 ◽

Cited By ~ 2

Author(s):

Felix A. Cervantes ◽

Juan M. Alvarez

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Green Peach Aphid ◽

Transmission Rate ◽

Solanum Tuberosum L ◽

Macrosiphum Euphorbiae ◽

Invasive Weed ◽

Potato Plants ◽

Tuber Necrosis

The complexity of the Potato virus Y (PVY) (Potyviridae: Potyvirus) pathosystem is affected by the presence of several virus strains that differ in their ability to produce tuber necrosis and by the presence of an alternate host that could increase the amount of inoculum in potato fields. Solanum sarrachoides (Sendtner) is an invasive weed from South America present in Pacific Northwest potato agro-ecosystems. It serves as reservoir of PVY and its most efficient vectors: the green peach aphid, Myzus persicae (Sulzer), and the potato aphid, Macrosiphum euphorbiae (Thomas). The role of S. sarracoides as vector and virus reservoir in PVY epidemiology was investigated through a series of laboratory and greenhouse experiments. We studied the symptoms produced in S. sarracoides upon infection with necrotic and non-necrotic strains of PVY and looked at the percentage of infection and titer accumulation of these strains. PVY infection in S. sarrachoides produced symptoms similar to those produced in PVY-infected potato plants. Mottling and yellowing were the main symptoms of infection observed in S. sarrachoides plants, especially by PVYO and PVYNTN infection. Greenhouse transmission studies revealed that PVY-infected S. sarrachoides increased the transmission rate of PVY necrotic strains by M. persicae. The necrotic strain PVYNTN reached higher titer in S. sarrachoides than in potato plants when compared to PVYO and PVYN:O These findings have broadened our understanding of the role and importance of S. sarrachoides in the PVY epidemiology in the potato ecosystems and could potentially be included in the development or optimization of virus management programs. Accepted for publication 15 March 2010. Published 26 May 2010.

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