Hydrogen production conditions from food waste by dark fermentation with Clostridium beijerinckii KCTC 1785

2008 ◽  
Vol 13 (4) ◽  
pp. 499-504 ◽  
Author(s):  
Jung Kon Kim ◽  
Le Nhat ◽  
Young Nam Chun ◽  
Si Wouk Kim
Keyword(s):  
Hydrogen Production ◽  
Food Waste ◽  
Dark Fermentation ◽  
Clostridium Beijerinckii ◽  
Production Conditions

Enhancement split-phase hydrogen production from food waste during dark fermentation: Protein substances degradation and transformation during hydrothermal pre-treatments

2019 ◽  
Vol 44 (32) ◽  
pp. 17334-17345 ◽  
Author(s):  
Xuan Jia ◽  
Mingxiao Li ◽  
Jinlong Zhu ◽  
Yonghai Jiang ◽  
Yong Wang ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Food Waste ◽  
Dark Fermentation

scholarly journals Enhancement of hydrogen production and energy recovery through electro-fermentation from the dark fermentation effluent of food waste

2020 ◽  
Vol 1 ◽  
pp. 100006 ◽  
Author(s):  
Xuan Jia ◽  
Mingxiao Li ◽  
Yong Wang ◽  
Yanan Wu ◽  
Lin Zhu ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Food Waste ◽  
Energy Recovery ◽  
Dark Fermentation

Batch dark fermentation from enzymatic hydrolyzed food waste for hydrogen production

2015 ◽  
Vol 191 ◽  
pp. 24-29 ◽  
Author(s):  
Wei Han ◽  
Min Ye ◽  
Ai Jun Zhu ◽  
Hong Ting Zhao ◽  
Yong Feng Li
Keyword(s):  
Hydrogen Production ◽  
Food Waste ◽  
Dark Fermentation

Enhanced hydrogen production from food waste dark fermentation by potassium ferrate pretreatment

2020 ◽  
Vol 27 (15) ◽  
pp. 18145-18156 ◽  
Author(s):  
Yan Kuang ◽  
Jianwei Zhao ◽  
Ying Gao ◽  
Chenggang Lu ◽  
Siyi Luo ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Food Waste ◽  
Dark Fermentation ◽  
Potassium Ferrate

scholarly journals Improving sustainable hydrogen production from green waste: [FeFe]-hydrogenases quantitative gene expression RT-qPCR analysis in presence of autochthonous consortia

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
M. Arizzi ◽  
S. Morra ◽  
G. Gilardi ◽  
M. Pugliese ◽  
M. L. Gullino ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hydrogen Production ◽  
Dark Fermentation ◽  
Clostridium Beijerinckii ◽  
Environmental Benefits ◽  
Added Value ◽  
Public Parks ◽  
Turnover Rates ◽  
High Turnover ◽  
Green Waste

Abstract Background Bio-hydrogen production via dark fermentation of low-value waste is a potent and simple mean of recovering energy, maximising the harvesting of reducing equivalents to produce the cleanest fuel amongst renewables. Following several position papers from companies and public bodies, the hydrogen economy is regaining interest, especially in combination with circular economy and the environmental benefits of short local supply chains, aiming at zero net emission of greenhouse gases (GHG). The biomasses attracting the largest interest are agricultural and urban green wastes (pruning of trees, collected leaves, grass clippings from public parks and boulevards), which are usually employed in compost production, with some concerns over the GHG emission during the process. Here, an alternative application of green wastes, low-value compost and intermediate products (partially composted but unsuitable for completing the process) is studied, pointing at the autochthonous microbial consortium as an already selected source of implementation for biomass degradation and hydrogen production. The biocatalysts investigated as mainly relevant for hydrogen production were the [FeFe]-hydrogenases expressed in Clostridia, given their very high turnover rates. Results Bio-hydrogen accumulation was related to the modulation of gene expression of multiple [FeFe]-hydrogenases from two strains (Clostridium beijerinckii AM2 and Clostridium tyrobutyricum AM6) isolated from the same waste. Reverse Transcriptase quantitative PCR (RT-qPCR) was applied over a period of 288 h and the RT-qPCR results showed that C. beijerinckii AM2 prevailed over C. tyrobutyricum AM6 and a high expression modulation of the 6 different [FeFe]-hydrogenase genes of C. beijerinckii in the first 23 h was observed, sustaining cumulative hydrogen production of 0.6 to 1.2 ml H2/g VS (volatile solids). These results are promising in terms of hydrogen yields, given that no pre-treatment was applied, and suggested a complex cellular regulation, linking the performance of dark fermentation with key functional genes involved in bio-H2 production in presence of the autochthonous consortium, with different roles, time, and mode of expression of the involved hydrogenases. Conclusions An applicative outcome of the hydrogenases genes quantitative expression analysis can be foreseen in optimising (on the basis of the acquired functional data) hydrogen production from a nutrient-poor green waste and/or low added value compost, in a perspective of circular bioeconomy.

Optimization of Hydrogen Production by Co-Culture of Clostridium beijerinckii and Rhodobacter sphaeroides Bacteria

2014 ◽  
Vol 93 ◽  
pp. 90-95 ◽  
Author(s):  
Roman Zagrodnik
Keyword(s):  
Hydrogen Production ◽  
Rhodobacter Sphaeroides ◽  
Hydrogen Generation ◽  
Nitrogen Sources ◽  
Dark Fermentation ◽  
Single Step ◽  
Clostridium Beijerinckii ◽  
Hydrogen Yield ◽  
Batch Tests ◽  
Pure Cultures

The biological methods of hydrogen generation have attracted a significant interest recently. In this work the hybrid system applying both dark fermentation bacteria in co-culture was tested. Objective of this work was to investigate the optimization of different parameters on co-culture of Clostridium beijerinckii DSM-791 and Rhodobacter sphaeroides O.U.001. The effect of glucose concentration (1–5 g/L), temperature and initial pH (6,5–7,5) was analyzed. Moreover the influence of organic nitrogen sources were tested for their capacity to support hydrogen production (yeast extract, peptone, glutamic acid). Fermentations were conducted in batch tests with glucose as sole substrate. Hydrogen production in mixed culture was compared with pure cultures. The process was greatly affected by pH and light/dark bacteria ratio. Liquid metabolites, namely acetic and butyric acids, from the dark fermentation step were the source of organic carbon for photosynthetic bacteria. This increased the hydrogen yield in comparison to single-step dark fermentation to over 4 mol H2/mol glucose. Obtained results showed that combination of photo and dark fermentation may increase hydrogen production and conversion efficiency of complex substrates or wastewaters.

Supercritical water gasification of food waste: Effect of parameters on hydrogen production

2020 ◽  
Vol 45 (29) ◽  
pp. 14744-14755 ◽  
Author(s):  
Wei Su ◽  
Changqing Cai ◽  
Ping Liu ◽  
Wei Lin ◽  
Baorui Liang ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Food Waste ◽  
Supercritical Water ◽  
Supercritical Water Gasification

scholarly journals Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
R. M. Silva ◽  
A. A. Abreu ◽  
A. F. Salvador ◽  
M. M. Alves ◽  
I. C. Neves ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Anaerobic Bacteria ◽  
Dark Fermentation ◽  
Inorganic Materials ◽  
Biohydrogen Production ◽  
High Rate ◽  
Continuous Reactor ◽  
Organic Loading Rate ◽  
Fermentation Products ◽  
Zeolite Type

AbstractThermophilic biohydrogen production by dark fermentation from a mixture (1:1) of C5 (arabinose) and C6 (glucose) sugars, present in lignocellulosic hydrolysates, and from Sargassum sp. biomass, is studied in this work in batch assays and also in a continuous reactor experiment. Pursuing the interest of studying interactions between inorganic materials (adsorbents, conductive and others) and anaerobic bacteria, the biological processes were amended with variable amounts of a zeolite type-13X in the range of zeolite/inoculum (in VS) ratios (Z/I) of 0.065–0.26 g g−1. In the batch assays, the presence of the zeolite was beneficial to increase the hydrogen titer by 15–21% with C5 and C6-sugars as compared to the control, and an increase of 27% was observed in the batch fermentation of Sargassum sp. Hydrogen yields also increased by 10–26% with sugars in the presence of the zeolite. The rate of hydrogen production increased linearly with the Z/I ratios in the experiments with C5 and C6-sugars. In the batch assay with Sargassum sp., there was an optimum value of Z/I of 0.13 g g−1 where the H2 production rate observed was the highest, although all values were in a narrow range between 3.21 and 4.19 mmol L−1 day−1. The positive effect of the zeolite was also observed in a continuous high-rate reactor fed with C5 and C6-sugars. The increase of the organic loading rate (OLR) from 8.8 to 17.6 kg m−3 day−1 of COD led to lower hydrogen production rates but, upon zeolite addition (0.26 g g−1 VS inoculum), the hydrogen production increased significantly from 143 to 413 mL L−1 day−1. Interestingly, the presence of zeolite in the continuous operation had a remarkable impact in the microbial community and in the profile of fermentation products. The effect of zeolite could be related to several properties, including the porous structure and the associated surface area available for bacterial adhesion, potential release of trace elements, ion-exchanger capacity or ability to adsorb different compounds (i.e. protons). The observations opens novel perspectives and will stimulate further research not only in biohydrogen production, but broadly in the field of interactions between bacteria and inorganic materials.

Sign in / Sign up

Export Citation Format

Share Document