Optimization of Hydrogen Production by Co-Culture of Clostridium beijerinckii and Rhodobacter sphaeroides Bacteria

2014 ◽  
Vol 93 ◽  
pp. 90-95 ◽  
Author(s):  
Roman Zagrodnik
Keyword(s):  
Hydrogen Production ◽  
Rhodobacter Sphaeroides ◽  
Hydrogen Generation ◽  
Nitrogen Sources ◽  
Dark Fermentation ◽  
Single Step ◽  
Clostridium Beijerinckii ◽  
Hydrogen Yield ◽  
Batch Tests ◽  
Pure Cultures

The biological methods of hydrogen generation have attracted a significant interest recently. In this work the hybrid system applying both dark fermentation bacteria in co-culture was tested. Objective of this work was to investigate the optimization of different parameters on co-culture of Clostridium beijerinckii DSM-791 and Rhodobacter sphaeroides O.U.001. The effect of glucose concentration (1–5 g/L), temperature and initial pH (6,5–7,5) was analyzed. Moreover the influence of organic nitrogen sources were tested for their capacity to support hydrogen production (yeast extract, peptone, glutamic acid). Fermentations were conducted in batch tests with glucose as sole substrate. Hydrogen production in mixed culture was compared with pure cultures. The process was greatly affected by pH and light/dark bacteria ratio. Liquid metabolites, namely acetic and butyric acids, from the dark fermentation step were the source of organic carbon for photosynthetic bacteria. This increased the hydrogen yield in comparison to single-step dark fermentation to over 4 mol H2/mol glucose. Obtained results showed that combination of photo and dark fermentation may increase hydrogen production and conversion efficiency of complex substrates or wastewaters.

scholarly journals Hydrogen Production from Coffee Mucilage in Dark Fermentation with Organic Wastes

Energies ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 71 ◽  
Author(s):  
Edilson Cárdenas ◽  
Arley Zapata-Zapata ◽  
Daehwan Kim
Keyword(s):  
Hydrogen Production ◽  
Manufacturing Industry ◽  
Nitrogen Sources ◽  
Bacterial Consortium ◽  
Dark Fermentation ◽  
Organic Wastes ◽  
Hydrogen Yield ◽  
Hydrogen Metabolism ◽  
Wholesale Market ◽  
Experimental Conditions

One of primary issues in the coffee manufacturing industry is the production of large amounts of undesirable residues, which include the pericarp (outer skin), pulp (outer mesocarp), parchment (endocarp), silver-skin (epidermis) and mucilage (inner mesocarp) that cause environmental problems due to toxic molecules contained therein. This study evaluated the optimal hydrogen production from coffee mucilage combined with organic wastes (wholesale market garbage) in a dark fermentation process. The supplementation of organic wastes offered appropriate carbon and nitrogen sources with further nutrients; it was positively effective in achieving cumulative hydrogen production. Three different ratios of coffee mucilage and organic wastes (8:2, 5:5, and 2:8) were tested in 30 L bioreactors using two-level factorial design experiments. The highest cumulative hydrogen volume of 25.9 L was gained for an 8:2 ratio (coffee mucilage: organic wastes) after 72 h, which corresponded to 1.295 L hydrogen/L substrates (0.248 mol hydrogen/mol hexose). Biochemical identification of microorganisms found that seven microorganisms were involved in the hydrogen metabolism. Further studies of anaerobic fermentative digestion with each isolated pure bacterium under similar experimental conditions reached a lower final hydrogen yield (up to 9.3 L) than the result from the non-isolated sample (25.9 L). Interestingly, however, co-cultivation of two identified microorganisms (Kocuria kristinae and Brevibacillus laterosporus), who were relatively highly associated with hydrogen production, gave a higher yield (14.7 L) than single bacterium inoculum but lower than that of the non-isolated tests. This work confirms that the re-utilization of coffee mucilage combined with organic wastes is practical for hydrogen fermentation in anaerobic conditions, and it would be influenced by the bacterial consortium involved.

scholarly journals Research on hydrogen generation from rapid hydrolysis of aluminum in sodium fluoride solution

2019 ◽  
Vol 118 ◽  
pp. 03048
Author(s):  
Changchun Li ◽  
Yuxin Wu
Keyword(s):  
Hydrogen Production ◽  
Kinetic Model ◽  
Sodium Fluoride ◽  
Hydrogen Generation ◽  
Hydrogen Yield ◽  
Shrinking Core Model ◽  
Fluoride Solution ◽  
Shrinking Core ◽  
Rapid Hydrolysis ◽  
Hydrolysis Of

Hydrogen generation from rapid hydrolysis of aluminum in sodium fluoride solution was investigated through a hydrolysis experiment. Rapid and instant hydrogen yield were observed using sodium fluoride as additive. The experimental results demonstrate that the increase of temperature and the amount of additives in a certain range will boost the hydrogen production. The amount of additives outside the range only has an effect on the rapid hydrolysis of the aluminum during the initial stage, but the total amount of hydrogen produced doesn’t increased significantly. Theoretical analysis of the effects of the mixing ratio and the temperature on the hydrogen production rates were performed using the shrinking core model and the kinetic model. The shrinking core model parameter a and k indicate the film change degree of porosity and thickness and the effect of time on the diffusion coefficient. the kinetic model is verified and the activation energy confirming hydrogen yield control by a molecular diffusion process. Correspondingly, mechanisms of Al corrosion in NaF solutions under low and high alkalinity were proposed, respectively.

Bio-electrochemical production of hydrogen and electricity from organic waste

2021 ◽  
Author(s):  
Giorgia De Gioannis ◽  
Alessandro Dell'Era ◽  
Aldo Muntoni ◽  
Mauro Pasquali ◽  
Alessandra Polettini ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Electron Flow ◽  
Dark Fermentation ◽  
Electrochemical Process ◽  
Integrated System ◽  
Galvanic Cell ◽  
Hydrogen Yield ◽  
Cell Coupling ◽  
Production Of Hydrogen ◽  
Acid Metabolites

Abstract This study investigated the performance of a novel integrated bio-electrochemical system for synergistic hydrogen production from a process combining a dark fermentation reactor and a galvanic cell. The operating principle of the system is based on the electrochemical conversion of protons released upon dissociation of the acid metabolites of the biological process and is mediated by the electron flow from the galvanic cell, coupling biochemical and electrochemical hydrogen production. Accordingly, the galvanic compartment also generates electricity. Four different experimental setups were designed to provide a preliminary assessment of the integrated bio-electrochemical process and identify the optimal configuration for further tests. Subsequently, dark fermentation of cheese whey was implemented both in a stand-alone biochemical reactor and in the integrated bio-electrochemical process. The integrated system achieved a hydrogen yield in the range 75.5 – 78.8 N LH2/kg TOC, showing a 3 times improvement over the biochemical process.

scholarly journals Biohydrogen production from sweet sorghum biomass using mixed acidogenic cultures and pure cultures of Ruminococcus Albus

2013 ◽  
Vol 9 (2) ◽  
pp. 144-151
Keyword(s):  
Hydrogen Production ◽  
Sweet Sorghum ◽  
Liquid Fraction ◽  
Continuous System ◽  
Extraction Process ◽  
Raw Material ◽  
Hydrogen Yield ◽  
Ruminococcus Albus ◽  
Fermentative Hydrogen Production ◽  
Pure Cultures

The present study focuses on the exploitation of sweet sorghum biomass as a source for hydrogen in continuous and batch systems. Sweet sorghum is an annual C4 plant of tropical origin, well-adapted to sub-tropical and temperate regions and highly productive in biomass. Sweet sorghum biomass is rich in readily fermentable sugars and thus it can be considered as an excellent raw material for fermentative hydrogen production. Extraction of free sugars from the sorghum stalks was achieved using water at 30°C. After the extraction process, a liquid fraction (sorghum extract), rich in sucrose, and a solid fraction (sorghum cellulosichemicellulosic residues), containing the cellulose and hemicelluloses, were obtained. Hydrogen production from sorghum extract was investigated using mixed acidogenic microbial cultures, coming from the indigenous sorghum microflora and Ruminococcus albus, an important, fibrolytic bacterium of the rumen. Hydrogen productivity of sorghum residues was assessed as well, using R. albus. The highest hydrogen yield obtained from sorghum extract fermented with mixed microbial cultures in continuous system was 0.86 mol hydrogen per mol of glucose consumed, at a hydraulic retention time of 12 hours. This corresponded to a hydrogen productivity of 10.4 l hydrogen per kg of sorghum biomass and was comparable with those obtained from batch experiments. On the other hand, the hydrogen yield obtained from sorghum extract treated with R. albus was as high as 2.1-2.6 mol hydrogen per mol of glucose consumed. Hydrogen productivity of sorghum residues fermented with R. albus reached 2.6 mol hydrogen per mol of glucose consumed. In total, the productivity of sorghum biomass (that of sorghum extract plus that of sorghum residues) could be 60 l hydrogen per kg of sorghum biomass if R. albus is used.

Use of Algae Biomass Obtained by Single-Step Mild Acid Hydrolysis in Hydrogen Production by the β-Glucosidase-Producing Clostridium beijerinckii Br21

2018 ◽  
Vol 11 (4) ◽  
pp. 1393-1402
Author(s):  
Bruna Constante Fonseca ◽  
Giovanni Dalbelo ◽  
Valeria Cress Gelli ◽  
Sibeli Carli ◽  
Luana Parras Meleiro ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Acid Hydrolysis ◽  
Single Step ◽  
Clostridium Beijerinckii ◽  
Mild Acid Hydrolysis ◽  
Algae Biomass ◽  
Mild Acid

Experimental Optimization of Green Hydrogen Production from Phototrophic Bacteria Rhodobacter sphaeroides

2019 ◽  
Vol 12 (2) ◽  
pp. 98-109
Author(s):  
Swetha Garimella ◽  
Archana Vimal ◽  
Ramchander Merugu ◽  
Awanish Kumar
Keyword(s):  
Hydrogen Production ◽  
Rhodobacter Sphaeroides ◽  
Energy Demand ◽  
Nitrogen Sources ◽  
Sewage Water ◽  
Quadratic Model ◽  
Carbon And Nitrogen ◽  
Cultural Conditions ◽  
Green Route ◽  
Production Of Hydrogen

Background and Objective: This study utilizes Rhodobacter sphaeroides bacteria for the photoproduction of hydrogen under various cultural conditions. R. sphaeroides was isolated from sewage water. We have examined different carbon and nitrogen sources for hydrogen production and further established the conditions for optimum hydrogen production by R. sphaeroides. Methods: The cumulative hydrogen produced by the bacteria at various intervals of time was measured using a Gas Chromatograph. Initially, by classical one factor at a time method, it was found that Benzoate and Glycine promote higher amounts of hydrogen production under anaerobic light conditions after 96 h. Results: The production was also observed to be enhanced in the presence of growth factors B12. Further, the Response Surface Methodology (RSM) was employed to optimize the hydrogen production. The first level of optimization was done using Box-Behnken Design (BBD) followed by Central Composite Design (CCD) method. The maximum production of hydrogen achieved by BBD and CCD was 6.8 ml/30 ml and 8.12 ml/30 ml, respectively. The significant model predicted is a quadratic model with R2 value 0.9459. Conclusion: Moreover, work presented here suggests an environment-friendly approach of harvesting H2, which could meet energy demand as clean fuel via the green route.

scholarly journals Rapid hydrogen generation from cotton wastes by mean of dark fermentation

2020 ◽  
Vol 2 (8) ◽  
Author(s):  
Gaweł Sołowski ◽  
Izabela Konkol ◽  
Marwa Shalaby ◽  
Adam Cenian
Keyword(s):  
Heat Shock ◽  
Hydrogen Production ◽  
Hydrogen Generation ◽  
Acidic Solution ◽  
Dark Fermentation ◽  
Oxygen Flow ◽  
Flow Rates ◽  
Cotton Waste ◽  
Lower Temperature ◽  
Mesophilic Conditions

AbstractDark fermentation of textile wastes is discussed in the paper. In the experiment cotton wastes were fermented. Before fermentation the cotton was hydrolyzed using 0.1 M HCl acidic solution. The inoculum was pretreated by means of heat shock for 0.5 h at 105 °C. The fermentation was carried out under mesophilic conditions at a load of 5 g VSS/L, and pH 5. Oxygen was added in small quantities during fermentation. The oxygen flow rates (OFR) were between 0.3 and 1.0 mL/h. The fermentation was carried out for a few days at temperatures between 40 and 43 °C. Hydrogenesis prevailed at the lower temperature (40 °C) and methanogenesis at the higher (43 °C). Conversion of cotton waste to methane (3.4%) was slightly higher than conversion to hydrogen (2.6%). The highest hydrogen production was obtained for OFR 0.8 mL/h and the percentage of hydrogen in biogas was 43%. At higher temperatures (43 °C) no hydrogen production was observed

scholarly journals Dark Fermentation of Sweet Sorghum Stalks, Cheese Whey and Cow Manure Mixture: Effect of pH, Pretreatment and Organic Load

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1017
Author(s):  
Margarita Andreas Dareioti ◽  
Aikaterini Ioannis Vavouraki ◽  
Konstantina Tsigkou ◽  
Constantina Zafiri ◽  
Michael Kornaros
Keyword(s):  
Fatty Acids ◽  
Hydrogen Production ◽  
Sweet Sorghum ◽  
Volatile Fatty Acids ◽  
Cheese Whey ◽  
Dark Fermentation ◽  
Organic Load ◽  
Anaerobic Sludge ◽  
Hydrogen Yield ◽  
Cow Manure

The aim of this study was to determine the optimal conditions for dark fermentation using agro-industrial liquid wastewaters mixed with sweet sorghum stalks (i.e., 55% sorghum, 40% cheese whey, and 5% liquid cow manure). Batch experiments were performed to investigate the effect of controlled pH (5.0, 5.5, 6.0, 6.5) on the production of bio-hydrogen and volatile fatty acids. According to the obtained results, the maximum hydrogen yield of 0.52 mol H2/mol eq. glucose was measured at pH 5.5 accompanied by the highest volatile fatty acids production, whereas similar hydrogen productivity was also observed at pH 6.0 and 6.5. The use of heat-treated anaerobic sludge as inoculum had a positive impact on bio-hydrogen production, exhibiting an increased yield of 1.09 mol H2/mol eq. glucose. On the other hand, the pretreated (ensiled) sorghum, instead of a fresh one, led to a lower hydrogen production, while the organic load decrease did not affect the process performance. In all experiments, the main fermentation end-products were volatile fatty acids (i.e., acetic, propionic, butyric), ethanol and lactic acid.

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