scholarly journals Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
R. M. Silva ◽  
A. A. Abreu ◽  
A. F. Salvador ◽  
M. M. Alves ◽  
I. C. Neves ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Anaerobic Bacteria ◽  
Dark Fermentation ◽  
Inorganic Materials ◽  
Biohydrogen Production ◽  
High Rate ◽  
Continuous Reactor ◽  
Organic Loading Rate ◽  
Fermentation Products ◽  
Zeolite Type

AbstractThermophilic biohydrogen production by dark fermentation from a mixture (1:1) of C5 (arabinose) and C6 (glucose) sugars, present in lignocellulosic hydrolysates, and from Sargassum sp. biomass, is studied in this work in batch assays and also in a continuous reactor experiment. Pursuing the interest of studying interactions between inorganic materials (adsorbents, conductive and others) and anaerobic bacteria, the biological processes were amended with variable amounts of a zeolite type-13X in the range of zeolite/inoculum (in VS) ratios (Z/I) of 0.065–0.26 g g−1. In the batch assays, the presence of the zeolite was beneficial to increase the hydrogen titer by 15–21% with C5 and C6-sugars as compared to the control, and an increase of 27% was observed in the batch fermentation of Sargassum sp. Hydrogen yields also increased by 10–26% with sugars in the presence of the zeolite. The rate of hydrogen production increased linearly with the Z/I ratios in the experiments with C5 and C6-sugars. In the batch assay with Sargassum sp., there was an optimum value of Z/I of 0.13 g g−1 where the H2 production rate observed was the highest, although all values were in a narrow range between 3.21 and 4.19 mmol L−1 day−1. The positive effect of the zeolite was also observed in a continuous high-rate reactor fed with C5 and C6-sugars. The increase of the organic loading rate (OLR) from 8.8 to 17.6 kg m−3 day−1 of COD led to lower hydrogen production rates but, upon zeolite addition (0.26 g g−1 VS inoculum), the hydrogen production increased significantly from 143 to 413 mL L−1 day−1. Interestingly, the presence of zeolite in the continuous operation had a remarkable impact in the microbial community and in the profile of fermentation products. The effect of zeolite could be related to several properties, including the porous structure and the associated surface area available for bacterial adhesion, potential release of trace elements, ion-exchanger capacity or ability to adsorb different compounds (i.e. protons). The observations opens novel perspectives and will stimulate further research not only in biohydrogen production, but broadly in the field of interactions between bacteria and inorganic materials.

scholarly journals Zeolite addition to improve biohydrogen production from dark fermentation of organic wastes

2020 ◽  
Author(s):  
Ana Rita M Silva ◽  
Angela A Abreu ◽  
Andreia F Salvador ◽  
Maria Madalena Alves ◽  
Isabel C Neves ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Microbial Community Composition ◽  
Dark Fermentation ◽  
Biohydrogen Production ◽  
Continuous Reactor ◽  
Batch Reactors ◽  
Biological Processes ◽  
Production Improvement ◽  
Volatile Solids ◽  
Zeolite Type

Abstract Background Hydrogen is a clean and renewable energy source that can be produced by biological processes, such as dark fermentation. However, hydrogen production yields are usually low.Results In this work, biohydrogen production from a mixture (1:1) of glucose and arabinose (4.4 g L -1 , in COD) was improved about 1.3 times in batch reactors, and increased 3 times in a continuous reactor (from 143 mL H2 L -1 d -1 to 430 mL H2 L -1 d -1 ), when zeolite type-13X was added. The presence of zeolite led to the stimulation of different metabolic pathways and to changes in the microbial community composition, which seems to be linked to hydrogen production improvement. The zeolite effect in dark fermentation was also verified for more complex substrates. Hydrogen production yield from Sargassum sp., was improved 1.4 times by the presence of zeolite (94.8 L H 2 Kg -1 Sargassum sp . Volatile Solids (VS)).Conclusions The results show that zeolite is suitable to improve biohydrogen production by dark fermentation.

Kinetics of Biohydrogen Production from Ozonated Palm Oil Mill Effluent Using C. butyricum and C. acetobutylicum Co-Culture

2012 ◽  
Vol 512-515 ◽  
pp. 1515-1519 ◽  
Author(s):  
Nipon Pisutpaisal ◽  
Saowaluck Hoasagul
Keyword(s):  
Hydrogen Production ◽  
Palm Oil ◽  
Biohydrogen Production ◽  
Palm Oil Mill Effluent ◽  
Batch Reactors ◽  
Fermentation Products ◽  
Ozone Pretreatment ◽  
Palm Oil Mill ◽  
Kinetics Of ◽  
Mesophilic Condition

Kinetics of mesophilic biohydrogen production from ozone-pretreated palm oil mill effluent (POME) using C. butyricum and C. acetobutylicum co-culture was investigated. All experiments were setup in 0.5-L batch reactors under mesophilic condition (37°C), pH 6, and POME concentration of 5,000-30,000 mg COD L-1. At the concentration of 15,000 mg COD L-1, maximum hydrogen production yield for non-ozone pretreated POME and ozone pretreated POME were 318 and 122 mL g-1 CODremoved, respectively. Acetic and butyric acids were dominant fermentation products in liquid phase. Ozone-pretreatment of POME showed no significant improvement on the hydrogen production by the co-culture.

scholarly journals Optimization of Biohydrogen Production with Biomechatronics

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Shao-Yi Hsia ◽  
Yu-Tuan Chou
Keyword(s):  
Hydrogen Production ◽  
Taguchi Method ◽  
Alternative Energy ◽  
Production Efficiency ◽  
Biological Effects ◽  
Dark Fermentation ◽  
Operating Conditions ◽  
Biohydrogen Production ◽  
Process Conditions ◽  
Ultrasonic Frequency

Massive utilization of petroleum and natural gas caused fossil fuel shortages. Consequently, a large amount of carbon dioxide and other pollutants are produced and induced environmental impact. Hydrogen is considered a clean and alternative energy source. It contains relatively high amount of energy compared with other fuels and by-product is water. In this study, the combination of ultrasonic mechanical and biological effects is utilized to increase biohydrogen production from dark fermentation bacteria. The hydrogen production is affected by many process conditions. For obtaining the optimal result, experimental design is planned using the Taguchi Method. Four controlling factors, the ultrasonic frequency, energy, exposure time, and starch concentration, are considered to calculate the highest hydrogen production by the Taguchi Method. Under the best operating conditions, the biohydrogen production efficiency of dark fermentation increases by 19.11%. Results have shown that the combination of ultrasound and biological reactors for dark fermentation hydrogen production outperforms the traditional biohydrogen production method. The ultrasonic mechanical effects in this research always own different significances on biohydrogen production.

scholarly journals Thermophilic Dark Fermentation of Sewage Sludge for Biohydrogen Production - Influence of pH

2018 ◽  
Vol 20 (3) ◽  
pp. 564-571
Keyword(s):  
Sewage Sludge ◽  
Hydrogen Production ◽  
Waste Water Treatment ◽  
Dark Fermentation ◽  
Biohydrogen Production ◽  
Pure Hydrogen ◽  
Constant Ph ◽  
Initial Ph ◽  
Sludge Waste ◽  
Almost All

<p>This study investigates the usability of sewage sludge, waste from a waste water treatment facility, at the stable thermophilic temperature and different pH conditions in the biohydrogen production by dark fermentation. Without the addition of a pure hydrogen producer and nutrient source, the effect of a different constant pH in the range of pH 4-9 on biohydrogen production using sewage sludge was compared with that of a different initial pH. It was understood from the results that biohydrogen production varies according to the characterization of sewage sludge. In the experiments, the lag time was insignificant (~2h). The maximum hydrogen production was achieved at pH 5 within the first 24-30 hours of fermentation (92894 mL m-3 H2). Therefore, it was determined that the higher digestion efficiencies of the sewage sludge were obtained at pH 5. In general, with the increase in methanogens in the medium, the hydrogen producing ability and hydrogen content of the sewage sludge gradually decreased. Hydrogen production at almost all the pH values after the third day was less than 1000 mL m-3.</p>

The application of an innovative continuous multiple tube reactor as a strategy to control the specific organic loading rate for biohydrogen production by dark fermentation

2015 ◽  
Vol 197 ◽  
pp. 201-207 ◽  
Author(s):  
Simone D. Gomes ◽  
Lucas T. Fuess ◽  
Eduardo D. Penteado ◽  
Shaiane D.M. Lucas ◽  
Jackeline T. Gotardo ◽  
...  
Keyword(s):  
Loading Rate ◽  
Dark Fermentation ◽  
Biohydrogen Production ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Tube Reactor

scholarly journals Effect of pH on the Economic Potential of Dark Fermentation Products from Used Disposable Nappies and Expired Food Products

2021 ◽  
Vol 11 (9) ◽  
pp. 4099
Author(s):  
Dimitris Zagklis ◽  
Marina Papadionysiou ◽  
Konstantina Tsigkou ◽  
Panagiota Tsafrakidou ◽  
Constantina Zafiri ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Hydrogen Production ◽  
Volatile Fatty Acids ◽  
Food Products ◽  
Treatment Method ◽  
Dark Fermentation ◽  
Biodiesel Production ◽  
Economic Potential ◽  
Fermentation Products ◽  
Ph Values

Used disposable nappies constitute a waste stream that has no established treatment method. The purpose of this study was the assessment of the dark fermentation of used disposable nappies and expired food products under different pH values. The biodegradable part of the used disposable nappies was recovered and co-fermented with expired food products originating from supermarkets. The recoverable economic potential of the process was examined for different volatile fatty acids exploitation schemes and process pH values. The process pH strongly affected the products, with optimum hydrogen production at pH 6 (4.05 NLH2/Lreactor), while the amount of produced volatile fatty acids was maximized at pH 7 (13.44 g/L). Hydrogen production was observed at pH as low as pH 4.5 (2.66 NLH2/Lreactor). The recoverable economic potential was maximized at two different pH values, with the first being pH 4.5 with minimum NaOH addition requirements (181, 138, and 296 EUR/ton VS of substrate for valorization of volatile fatty acids through microbial fuel cell, biodiesel production, and anaerobic digestion, respectively) and the second being pH 6, where the hydrogen production was maximized with the simultaneous production of high amounts of volatile fatty acids (191, 142, and 339 EUR/ton VS of substrate respectively).

Properties of Anaerobic Activated Sludge in Biohydrogen Production Reactor

2014 ◽  
Vol 884-885 ◽  
pp. 503-506
Author(s):  
Zhi Qin ◽  
Guang Yu Bai ◽  
Qi Zhang ◽  
Yong Yan Cui ◽  
Chao Yu Zhang
Keyword(s):  
Hydrogen Production ◽  
Activated Sludge ◽  
Retention Time ◽  
Hydraulic Retention Time ◽  
Loading Rate ◽  
Biohydrogen Production ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Mixture Volume ◽  
Total Mixture

For the properties of activated sludge studies, three kinds of activated sludge were obtained from continuous hydrogen production reactor. Activated sludge was got under organic loading rate (OLR) of 3, 7 and 25 kgCOD/m3·d condition, respectively. Sedimentation performance and activities of sludge were investigated. When OLR was 3 kgCOD/m3·d, activated sludge showed good sedimentation performance. After 30 minutes sedimentation, the volume of activated sludge in total mixture volume was about 39%. When OLR was 7 and 25kgCOD/m3·d, after 30 minutes sedimentation, the volume of activated sludge in total mixture volume was 80% and 83%, respectively. The increase of biomass is the main reason for increase of sedimentation performance. MLVSS/MLSS of activated sludge was 37.7% and 79.6% under OLR of 3 and 25kgCOD/m3·d condition, therefore, activities of activated sludge was high under high OLR condition. Since sedimentation performance of sludge is high under high OLR condition, hydraulic retention time should controlled carefully in engineering operation.

scholarly journals Enhancement of biohydrogen production from the aquatic weed Pistia stratiotes through a dark fermentation process

2019 ◽  
Author(s):  
◽  
Nonsikelelo Precios Mthethwa
Keyword(s):  
Hydrogen Production ◽  
Fermentation Process ◽  
Dark Fermentation ◽  
Biohydrogen Production ◽  
Pistia Stratiotes ◽  
Aquatic Weed ◽  
Operational Conditions ◽  
Bacillus Spp ◽  
Pre Treatment ◽  
Clostridium Spp

Aquatic weeds are well known for their fast growth rate and high carbohydrate content that can be easily hydrolysed into fermentable sugars. This study was aimed at the utilization of an indigenous aquatic weed, Pistia stratiotes for biohydrogen production through the dark fermentation process. Characterization of the biomass, effect of pre–treatment methods on biomass hydrolysis, effect of reactor operational conditions and type of inoculum on enhancing hydrogen production potential of P. stratiotes was assessed. Physical and chemical pre–treatments were employed on P. stratiotes biomass to increase digestibility and to achieve high conversion rates of fermentable sugars. The highest sugar yield of 139± 0.8 mg/g was obtained when the oven dried biomass was subjected to H2SO4 (2.5%) pre– treatment followed by autoclaving at 121°C for 30 min. Biohydrogen production under different operational conditions was thereafter optimized using One–factor–at–a–time (OFAT) batch experiments in 120 mL serum bottles. A maximum hydrogen yield (HY) of 2.46 ± 0.14 mol-H2/mol-glucose (3.51 ± 0.20 mg-H2/g-dry weight) and 2.75 ± 0.07 mL h-1 hydrogen production rate was observed under optimized conditions (pH 5.5, Temp 35°C, S/X: 1.0 g-COD/g-VSS and HRT 8 h). The organic mass balance (92 – 96%) and electron– equivalent balance (92 – 98%) further indicated the reliability of the obtained fermentation data. Assessment of microbial activity was achieved using molecular techniques such as quantitative polymerase chain reaction (qPCR) targeting both 16s rRNA (of Clostridium spp., Bacillus spp., and Enterobacter spp.) and the functional hydrogenase gene (hydA). The highest gene activity of hydrogenase was noted at pH of 5.5 with 2.53×104 copies/ng-DNA compared to low pH: 4.5 (6.95 × 103 copies/ng-DNA) and high pH: 8.5 (7.77×103 copies/ng- DNA). A similar trend was also observed for the species containing a highly active hydrogenase (i.e. Clostridium spp., Bacillus spp., and Enterobacter spp.). During the optimum reactor conditions, three hydrogen producing bacterial strains Bacillus cereus and Enterobacter cloacae were successfully isolated. These isolates were used as inoculums for the pure culture studies and achieved HYs of 2.2, 1.10 and 1.97 mol-H2/mol-glucose respectively under optimized fermentation conditions. However, the thermally treated mixed culture displayed a marginally higher HY (2.46 mol-H2/mol-glucose) compared to the pure culture used alone. Furthermore, the cost estimation indicated a potential and economically feasible for biotransformation of P. stratiotes to hydrogen energy. In conclusion, the results from this study has revealed the potential of employing P. stratiotes biomass for biohydrogen production. The results also indicated the importance of employing suitable pre–treatment methods, operating conditions as well as inoculum types for enhanced hydrogen production using P. stratiotes.

scholarly journals Bio-hydrogen Production from Beer Wastewater in an Internal Circulation (IC) Reactor

2015 ◽  
Vol 8 (1) ◽  
pp. 81-85
Author(s):  
Su Caili ◽  
Ji baojie ◽  
Wang Qun ◽  
Zhu Lingfeng
Keyword(s):  
Hydrogen Production ◽  
Ph Value ◽  
Granular Sludge ◽  
Production Equipment ◽  
Organic Loading Rate ◽  
Internal Circulation ◽  
Fermentation Products ◽  
High Hydrogen ◽  
Fermentative Hydrogen Production ◽  
Liquid Products

An internal circulation (IC) using hydrogen-producing anaerobic granular sludge as seed sludge and beer wastewater as substrate was employed to evaluate the effect of hydrogen production and the performance of reactor. Running at the temperature of 35±1 °C, the pH value of influent controlled is 5.5 and organic loading rate (OLR) from 30 kg COD/(m3●d) to 42 kg COD/(m3●d), the IC reactor presents a high hydrogen production ability as the hydrogen production rate (HPR) maximized at 6.0-6.83 m3/(m3●d). Hydrogen volume content was estimated to be 42-46% of the total biogas and the biogas was free of methane throughout the study. COD removal efficiency could reach 20-30% and the dissolved fermentation products were predominated by ethanol with the concentration of 900-950 mg/L, which accounts for 45%- 56% of the total liquid products. These values may imply that the IC reactor is a kind of feasible fermentative hydrogen production equipment.

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